MiR-203a is differentially expressed during branching morphogenesis and EMT in breast progenitor cells and is a repressor of peroxidasin.

• Published in: Mechanisms of Development
• Main Authors: Briem, Eirikur, Budkova, Zuzana, Sigurdardottir, Anna Karen, Hilmarsdottir, Bylgja, Kricker, Jennifer, Timp, Winston, Magnusson, Magnus Karl, Traustadottir, Gunnhildur Asta, Gudjonsson, Thorarinn
• Other Authors: 1 Stem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Iceland. 2 Stem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Iceland; Department of Tumor Biology, The Norwegian Radium Hospital, Oslo, Norway. 3 Department of Biomedical Engineering, Johns Hopkins University, USA. 4 Department of Laboratory Hematology, Landspitali - University Hospital, Iceland; Department of Pharmacology and Toxicology, Faculty of Medicine, School of Health Sciences, University of Iceland, Iceland. 5 Stem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Iceland; Department of Laboratory Hematology, Landspitali - University Hospital, Iceland. Electronic address: [e-mail].
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier Science 2019
• Subjects: Gen; Morphogenesis; Iceland
• Online Access: http://hdl.handle.net/2336/620871; https://doi.org/10.1016/j.mod.2018.11.002

To access publisher's full text version of this article click on the hyperlink below MicroRNAs regulate developmental events such as branching morphogenesis, epithelial to mesenchymal transition (EMT) and its reverse process mesenchymal to epithelial transition (MET). In this study, we performed small RNA sequencing of a breast epithelial progenitor cell line (D492), and its mesenchymal derivative (D492M) cultured in three-dimensional microenvironment. Among the most downregulated miRNAs in D492M was miR-203a, a miRNA that plays an important role in epithelial differentiation. Increased expression of miR-203a was seen in D492, concomitant with increased complexity of branching. When miR-203a was overexpressed in D492M, a partial reversion towards epithelial phenotype was seen. Gene expression analysis of D492M and D492M Landspitali University Hospital Science Fund University of Iceland Research Fund Icelandic Science and Technology Policy - Grant of Excellence