Blood-gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation.

• Published in: Sleep
• Main Authors: Arnardottir, Erna S, Nikonova, Elena V, Shockley, Keith R, Podtelezhnikov, Alexei A, Anafi, Ron C, Tanis, Keith Q, Maislin, Greg, Stone, David J, Renger, John J, Winrow, Christopher J, Pack, Allan I
• Other Authors: Univ Penn, Perelman Sch Med, Ctr Sleep & Circadian Neurobiol, Philadelphia, PA 19104 USA, Univ Penn, Perelman Sch Med, Dept Med, Div Sleep Med, Philadelphia, PA 19104 USA, Landspitali Natl Univ Hosp, Dept Resp Med & Sleep, IS-108 Reykjavik, Iceland, Univ Iceland, Fac Med, IS-101 Reykjavik, Iceland, Merck Res Labs, Dept Exploratory & Translat Sci, West Point, PA USA, NIEHS, Biostat Branch, NIH, Res Triangle Pk, NC 27709 USA, Merck Res Labs, Dept Neurosci, West Point, PA USA
• Format: Article in Journal/Newspaper
• Language: English
• Published: Amer Acad Sleep Medicine 2015
• Subjects: Svefntruflanir; Erfðagreining; Lífritmi; Circadian Rhythm/physiology*; Gene Expression Profiling; Microarray Analysis; Sleep Deprivation; Adult; Attention/physiology; Biological Markers; Female; Humans; Individuality; Male; Psychomotor Performance; Sleep/physiology; Sleep Deprivation/blood*; Sleep Deprivation/genetics*; Sleep Deprivation/physiopathology; Time Factors; Wakefulness/physiology; Iceland
• Online Access: http://hdl.handle.net/2336/337924; https://doi.org/10.5665/sleep.4064

To access publisher's full text version of this article click on the hyperlink at the bottom of the page To address whether changes in gene expression in blood cells with sleep loss are different in individuals resistant and sensitive to sleep deprivation. Blood draws every 4 h during a 3-day study: 24-h normal baseline, 38 h of continuous wakefulness and subsequent recovery sleep, for a total of 19 time-points per subject, with every 2-h psychomotor vigilance task (PVT) assessment when awake. Sleep laboratory. Fourteen subjects who were previously identified as behaviorally resistant (n = 7) or sensitive (n = 7) to sleep deprivation by PVT. Thirty-eight hours of continuous wakefulness. We found 4,481 unique genes with a significant 24-h diurnal rhythm during a normal sleep-wake cycle in blood (false discovery rate [FDR] < 5%). Biological pathways were enriched for biosynthetic processes during sleep. After accounting for circadian effects, two genes (SREBF1 and CPT1A, both involved in lipid metabolism) exhibited small, but significant, linear changes in expression with the duration of sleep deprivation (FDR < 5%). The main change with sleep deprivation was a reduction in the amplitude of the diurnal rhythm of expression of normally cycling probe sets. This reduction was noticeably higher in behaviorally resistant subjects than sensitive subjects, at any given P value. Furthermore, blood cell type enrichment analysis showed that the expression pattern difference between sensitive and resistant subjects is mainly found in cells of myeloid origin, such as monocytes. Individual differences in behavioral effects of sleep deprivation are associated with differences in diurnal amplitude of gene expression for genes that show circadian rhythmicity. NIH/ HL94307 Eimskip Fund of the University of Iceland Merck Research Laboratories (Merck Co., Inc.) National Center for Research Resources UL1RR024134 Intramural Research Program of the NIH, National Institute of Environmental Health Sciences ZIA ...