Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis.
To access publisher's full text version of this article. Please click on the hyperlink in Additional Links field. Dietary n-3 polyunsaturated fatty acids (PUFA) influence the inductive phase of inflammation but less is known about their effects on the resolution phase. This study examined the e...
Published in: | The Journal of Nutritional Biochemistry |
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Online Access: | http://hdl.handle.net/2336/317446 https://doi.org/10.1016/j.jnutbio.2013.03.005 |
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/317446 2023-05-15T16:52:20+02:00 Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. Tomasdottir, Valgerdur Vikingsson, Arnor Freysdottir, Jona Hardardottir, Ingibjorg Univ Iceland, Biomed Ctr, Dept Biochem & Mol Biol, IS-101 Reykjavik, Iceland Univ Iceland, Biomed Ctr, Dept Immunol, Fac Med, IS-101 Reykjavik, Iceland Landspitali, Ctr Rheumatol Res, Reykjavik, Iceland Landspitali, Dept Immunol, Reykjavik, Iceland 2014-05-26 http://hdl.handle.net/2336/317446 https://doi.org/10.1016/j.jnutbio.2013.03.005 en eng http://dx.doi.org/10.1016/j.jnutbio.2013.03.005 http://www.sciencedirect.com/science/article/pii/S0955286313000764 J. Nutr. Biochem. 2013, 24(10):1758-65 1873-4847 23769761 doi:10.1016/j.jnutbio.2013.03.005 http://hdl.handle.net/2336/317446 Journal of nutritional biochemistry Archived with thanks to The Journal of nutritional biochemistry National Consortium - Landsaðgangur Animals Chemokine CCL11 Chemokine CXCL1 Dietary Fats Unsaturated Fatty Acids Omega-3 Female Fish Oils Granulocyte Colony-Stimulating Factor Inflammation Interleukin-6 Macrophages Peritoneal Mice Inbred C57BL Peritonitis Receptors Serum Albumin Bovine Transforming Growth Factor beta Article 2014 ftlandspitaliuni https://doi.org/10.1016/j.jnutbio.2013.03.005 2022-05-29T08:21:56Z To access publisher's full text version of this article. Please click on the hyperlink in Additional Links field. Dietary n-3 polyunsaturated fatty acids (PUFA) influence the inductive phase of inflammation but less is known about their effects on the resolution phase. This study examined the effects of dietary fish oil on induction and resolution of antigen-induced inflammation in mice. Mice were fed a control diet with or without 2.8% fish oil, immunized twice with methylated BSA (mBSA) and inflammation induced by intraperitoneal injection of mBSA. Prior to and at different time points after mBSA administration, peritoneal cells were analyzed and expression of surface molecules determined by flow cytometry. Concentration of chemokines, cytokines and soluble cytokine receptors was determined by ELISA. Mice fed the fish oil diet had fewer peritoneal neutrophils, shorter resolution interval and lower levels of pro-inflammatory cytokines and chemokines than mice fed the control diet. In mice fed the fish oil diet there was an early peak in peritoneal levels of the immunosuppressive molecules sIL-6R and TGF-β, that was not seen in mice fed the control diet. In the resolution phase, peritoneal macrophages from mice fed the fish oil diet expressed more of the atypical chemokine receptor D6 and peritoneal TGF-β levels were higher than that in mice fed the control diet. Furthermore, in the late-resolution phase there were more peritoneal eosinophils and macrophages in mice fed the fish oil diet than in mice fed the control diet. These results demonstrate a suppressive effect of n-3 PUFA on the inductive phase of inflammation and indicate an enhancing effect of n-3 PUFA on resolution of inflammation. Icelandic Research Fund University of Iceland Research Fund Landspitali University Hospital Research Fund Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive The Journal of Nutritional Biochemistry 24 10 1758 1765 |
institution |
Open Polar |
collection |
Hirsla - Landspítali University Hospital research archive |
op_collection_id |
ftlandspitaliuni |
language |
English |
topic |
Animals Chemokine CCL11 Chemokine CXCL1 Dietary Fats Unsaturated Fatty Acids Omega-3 Female Fish Oils Granulocyte Colony-Stimulating Factor Inflammation Interleukin-6 Macrophages Peritoneal Mice Inbred C57BL Peritonitis Receptors Serum Albumin Bovine Transforming Growth Factor beta |
spellingShingle |
Animals Chemokine CCL11 Chemokine CXCL1 Dietary Fats Unsaturated Fatty Acids Omega-3 Female Fish Oils Granulocyte Colony-Stimulating Factor Inflammation Interleukin-6 Macrophages Peritoneal Mice Inbred C57BL Peritonitis Receptors Serum Albumin Bovine Transforming Growth Factor beta Tomasdottir, Valgerdur Vikingsson, Arnor Freysdottir, Jona Hardardottir, Ingibjorg Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. |
topic_facet |
Animals Chemokine CCL11 Chemokine CXCL1 Dietary Fats Unsaturated Fatty Acids Omega-3 Female Fish Oils Granulocyte Colony-Stimulating Factor Inflammation Interleukin-6 Macrophages Peritoneal Mice Inbred C57BL Peritonitis Receptors Serum Albumin Bovine Transforming Growth Factor beta |
description |
To access publisher's full text version of this article. Please click on the hyperlink in Additional Links field. Dietary n-3 polyunsaturated fatty acids (PUFA) influence the inductive phase of inflammation but less is known about their effects on the resolution phase. This study examined the effects of dietary fish oil on induction and resolution of antigen-induced inflammation in mice. Mice were fed a control diet with or without 2.8% fish oil, immunized twice with methylated BSA (mBSA) and inflammation induced by intraperitoneal injection of mBSA. Prior to and at different time points after mBSA administration, peritoneal cells were analyzed and expression of surface molecules determined by flow cytometry. Concentration of chemokines, cytokines and soluble cytokine receptors was determined by ELISA. Mice fed the fish oil diet had fewer peritoneal neutrophils, shorter resolution interval and lower levels of pro-inflammatory cytokines and chemokines than mice fed the control diet. In mice fed the fish oil diet there was an early peak in peritoneal levels of the immunosuppressive molecules sIL-6R and TGF-β, that was not seen in mice fed the control diet. In the resolution phase, peritoneal macrophages from mice fed the fish oil diet expressed more of the atypical chemokine receptor D6 and peritoneal TGF-β levels were higher than that in mice fed the control diet. Furthermore, in the late-resolution phase there were more peritoneal eosinophils and macrophages in mice fed the fish oil diet than in mice fed the control diet. These results demonstrate a suppressive effect of n-3 PUFA on the inductive phase of inflammation and indicate an enhancing effect of n-3 PUFA on resolution of inflammation. Icelandic Research Fund University of Iceland Research Fund Landspitali University Hospital Research Fund |
author2 |
Univ Iceland, Biomed Ctr, Dept Biochem & Mol Biol, IS-101 Reykjavik, Iceland Univ Iceland, Biomed Ctr, Dept Immunol, Fac Med, IS-101 Reykjavik, Iceland Landspitali, Ctr Rheumatol Res, Reykjavik, Iceland Landspitali, Dept Immunol, Reykjavik, Iceland |
format |
Article in Journal/Newspaper |
author |
Tomasdottir, Valgerdur Vikingsson, Arnor Freysdottir, Jona Hardardottir, Ingibjorg |
author_facet |
Tomasdottir, Valgerdur Vikingsson, Arnor Freysdottir, Jona Hardardottir, Ingibjorg |
author_sort |
Tomasdottir, Valgerdur |
title |
Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. |
title_short |
Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. |
title_full |
Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. |
title_fullStr |
Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. |
title_full_unstemmed |
Dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. |
title_sort |
dietary fish oil reduces the acute inflammatory response and enhances resolution of antigen-induced peritonitis. |
publishDate |
2014 |
url |
http://hdl.handle.net/2336/317446 https://doi.org/10.1016/j.jnutbio.2013.03.005 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://dx.doi.org/10.1016/j.jnutbio.2013.03.005 http://www.sciencedirect.com/science/article/pii/S0955286313000764 J. Nutr. Biochem. 2013, 24(10):1758-65 1873-4847 23769761 doi:10.1016/j.jnutbio.2013.03.005 http://hdl.handle.net/2336/317446 Journal of nutritional biochemistry |
op_rights |
Archived with thanks to The Journal of nutritional biochemistry National Consortium - Landsaðgangur |
op_doi |
https://doi.org/10.1016/j.jnutbio.2013.03.005 |
container_title |
The Journal of Nutritional Biochemistry |
container_volume |
24 |
container_issue |
10 |
container_start_page |
1758 |
op_container_end_page |
1765 |
_version_ |
1766042500825350144 |