Two circulating neutrophil populations in acute inflammation in mice.
Recent studies indicate that neutrophils are heterogeneous and may have an immunosuppressive role in addition to their well-known phagocytic and bactericidal function. This study examined neutrophil subpopulations in the circulation, peritoneum, spleen and bone marrow from mice at various time point...
Published in: | Inflammation Research |
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Online Access: | http://hdl.handle.net/2336/295046 https://doi.org/10.1007/s00011-012-0484-0 |
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/295046 2023-05-15T16:50:57+02:00 Two circulating neutrophil populations in acute inflammation in mice. Arnardottir, Hildur H Freysdottir, Jona Hardardottir, Ingibjorg Department of Biochemistry and Molecular Biology, University of Iceland, Vatnsmyrarvegur 16, IS-101, Reykjavik, Iceland. 2013-07-02 http://hdl.handle.net/2336/295046 https://doi.org/10.1007/s00011-012-0484-0 en eng Springer http:dx.doi.org/10.1007/s00011-012-0484-0 Inflamm. Res. 2012, 61(9):931-9 1420-908X 22585035 doi:10.1007/s00011-012-0484-0 http://hdl.handle.net/2336/295046 Inflammation research : official journal of the European Histamine Research Society . [et al.] Archived with thanks to Inflammation research : official journal of the European Histamine Research Society . [et al.] National Consortium - Landsaðgangur Animals Ascitic Fluid Cell Size Cytokines Female Inflammation Leukocyte Count Lipopolysaccharides Mice Inbred C57BL Neutrophils Article 2013 ftlandspitaliuni https://doi.org/10.1007/s00011-012-0484-0 2022-05-29T08:21:49Z Recent studies indicate that neutrophils are heterogeneous and may have an immunosuppressive role in addition to their well-known phagocytic and bactericidal function. This study examined neutrophil subpopulations in the circulation, peritoneum, spleen and bone marrow from mice at various time points after induction of acute inflammation. MATERIAL, TREATMENT AND METHODS: Female C57BL/6 mice were injected intraperitoneally with lipopolysaccharide (LPS). Blood, peritoneal, spleen and bone marrow cells were collected and counted and expression of surface molecules and chemokine receptors analyzed with flow cytometry. Chemokine and cytokine concentrations in serum and peritoneal fluid were determined by ELISA. Neutrophil numbers in the circulation decreased following administration of LPS but reached similar numbers to those prior to inflammation at 8 h. At that time point, two distinct neutrophil populations were present in the circulation. These two neutrophil populations differed in size, granularity and expression of CD11b and Ly6G. Few neutrophils were recruited into the peritoneum until 24 h after administration of LPS at a time when the neutrophils in the circulation had increased their expression of the chemokine receptor CXCR2. Induction of acute inflammation leads to the appearance of two circulating neutrophil subpopulations, which may differ in their activation state and function. Icelandic Research Fund University of Iceland Research Fund Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive Inflammation Research 61 9 931 939 |
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Hirsla - Landspítali University Hospital research archive |
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English |
topic |
Animals Ascitic Fluid Cell Size Cytokines Female Inflammation Leukocyte Count Lipopolysaccharides Mice Inbred C57BL Neutrophils |
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Animals Ascitic Fluid Cell Size Cytokines Female Inflammation Leukocyte Count Lipopolysaccharides Mice Inbred C57BL Neutrophils Arnardottir, Hildur H Freysdottir, Jona Hardardottir, Ingibjorg Two circulating neutrophil populations in acute inflammation in mice. |
topic_facet |
Animals Ascitic Fluid Cell Size Cytokines Female Inflammation Leukocyte Count Lipopolysaccharides Mice Inbred C57BL Neutrophils |
description |
Recent studies indicate that neutrophils are heterogeneous and may have an immunosuppressive role in addition to their well-known phagocytic and bactericidal function. This study examined neutrophil subpopulations in the circulation, peritoneum, spleen and bone marrow from mice at various time points after induction of acute inflammation. MATERIAL, TREATMENT AND METHODS: Female C57BL/6 mice were injected intraperitoneally with lipopolysaccharide (LPS). Blood, peritoneal, spleen and bone marrow cells were collected and counted and expression of surface molecules and chemokine receptors analyzed with flow cytometry. Chemokine and cytokine concentrations in serum and peritoneal fluid were determined by ELISA. Neutrophil numbers in the circulation decreased following administration of LPS but reached similar numbers to those prior to inflammation at 8 h. At that time point, two distinct neutrophil populations were present in the circulation. These two neutrophil populations differed in size, granularity and expression of CD11b and Ly6G. Few neutrophils were recruited into the peritoneum until 24 h after administration of LPS at a time when the neutrophils in the circulation had increased their expression of the chemokine receptor CXCR2. Induction of acute inflammation leads to the appearance of two circulating neutrophil subpopulations, which may differ in their activation state and function. Icelandic Research Fund University of Iceland Research Fund |
author2 |
Department of Biochemistry and Molecular Biology, University of Iceland, Vatnsmyrarvegur 16, IS-101, Reykjavik, Iceland. |
format |
Article in Journal/Newspaper |
author |
Arnardottir, Hildur H Freysdottir, Jona Hardardottir, Ingibjorg |
author_facet |
Arnardottir, Hildur H Freysdottir, Jona Hardardottir, Ingibjorg |
author_sort |
Arnardottir, Hildur H |
title |
Two circulating neutrophil populations in acute inflammation in mice. |
title_short |
Two circulating neutrophil populations in acute inflammation in mice. |
title_full |
Two circulating neutrophil populations in acute inflammation in mice. |
title_fullStr |
Two circulating neutrophil populations in acute inflammation in mice. |
title_full_unstemmed |
Two circulating neutrophil populations in acute inflammation in mice. |
title_sort |
two circulating neutrophil populations in acute inflammation in mice. |
publisher |
Springer |
publishDate |
2013 |
url |
http://hdl.handle.net/2336/295046 https://doi.org/10.1007/s00011-012-0484-0 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http:dx.doi.org/10.1007/s00011-012-0484-0 Inflamm. Res. 2012, 61(9):931-9 1420-908X 22585035 doi:10.1007/s00011-012-0484-0 http://hdl.handle.net/2336/295046 Inflammation research : official journal of the European Histamine Research Society . [et al.] |
op_rights |
Archived with thanks to Inflammation research : official journal of the European Histamine Research Society . [et al.] National Consortium - Landsaðgangur |
op_doi |
https://doi.org/10.1007/s00011-012-0484-0 |
container_title |
Inflammation Research |
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61 |
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9 |
container_start_page |
931 |
op_container_end_page |
939 |
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1766041069858848768 |