Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Listeria spp. and Listeria monocytogenes contamination of cold-smoked salmon (n=125) and its processing environment (n=522) were evaluated during surveys conducted in 1997-1998 and 2001 as...
Published in: | International Journal of Food Microbiology |
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Online Access: | http://hdl.handle.net/2336/2862 https://doi.org/10.1016/j.ijfoodmicro.2004.08.023 |
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/2862 2023-05-15T16:48:32+02:00 Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis Gudmundsdottir, Sigrun Gudbjornsdottir, Birna Lauzon, Helene L Einarsson, Hjorleifur Kristinsson, Karl G Kristjansson, Mar 2005-05-01 YES http://hdl.handle.net/2336/2862 https://doi.org/10.1016/j.ijfoodmicro.2004.08.023 en eng Elsevier Science Publishers http://www.sciencedirect.com/science/article/B6T7K-4FDMYJ6-1/2/641c540e18e622e5cbbd11c63a4fbee9 Int. J. Food Microbiol. 2005, 101(1):41-51 0168-1605 15878405 doi:10.1016/j.ijfoodmicro.2004.08.023 http://hdl.handle.net/2336/2862 International journal of food microbiology Animals DNA Bacterial Electrophoresis Gel Pulsed-Field Environmental Microbiology Food Contamination Food Handling Food-Processing Industry Iceland Incidence Listeria monocytogenes Salmon Seafood Serotyping Article 2005 ftlandspitaliuni https://doi.org/10.1016/j.ijfoodmicro.2004.08.023 2022-05-29T08:20:50Z To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Listeria spp. and Listeria monocytogenes contamination of cold-smoked salmon (n=125) and its processing environment (n=522) were evaluated during surveys conducted in 1997-1998 and 2001 as well as in samples of final products analysed in 2001. The overall frequencies of Listeria spp. and L. monocytogenes in samples from all sources were 15.1% and 11.3%, respectively, but the incidence of L. monocytogenes in cold-smoked salmon final products was only 4%. A total of 201 L. monocytogenes isolates were characterised by Pulsed-Field Gel Electrophoresis (PFGE) in order to trace L. monocytogenes contamination in the processing plants. The combination of AscI and ApaI macrorestriction patterns yielded 24 different pulsotypes in 6 plants. One pulsotype observed by AscI restriction digestion comprised 148 of the 167 typed isolates from two processing plants. Two other pulsotypes predominated in samples from raw material, processing environments and final products. The results indicate that raw material, floors, and drains are potential sources of the L. monocytogenes found on cold-smoked salmon products. This highlights the need to readdress the design and cleaning of processing plants and equipment, and staff behavior. Hindering the introduction into and spread of the organism through the processing environment is necessary to avoid jeopardizing safety of the final product. Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive International Journal of Food Microbiology 101 1 41 51 |
institution |
Open Polar |
collection |
Hirsla - Landspítali University Hospital research archive |
op_collection_id |
ftlandspitaliuni |
language |
English |
topic |
Animals DNA Bacterial Electrophoresis Gel Pulsed-Field Environmental Microbiology Food Contamination Food Handling Food-Processing Industry Iceland Incidence Listeria monocytogenes Salmon Seafood Serotyping |
spellingShingle |
Animals DNA Bacterial Electrophoresis Gel Pulsed-Field Environmental Microbiology Food Contamination Food Handling Food-Processing Industry Iceland Incidence Listeria monocytogenes Salmon Seafood Serotyping Gudmundsdottir, Sigrun Gudbjornsdottir, Birna Lauzon, Helene L Einarsson, Hjorleifur Kristinsson, Karl G Kristjansson, Mar Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis |
topic_facet |
Animals DNA Bacterial Electrophoresis Gel Pulsed-Field Environmental Microbiology Food Contamination Food Handling Food-Processing Industry Iceland Incidence Listeria monocytogenes Salmon Seafood Serotyping |
description |
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Listeria spp. and Listeria monocytogenes contamination of cold-smoked salmon (n=125) and its processing environment (n=522) were evaluated during surveys conducted in 1997-1998 and 2001 as well as in samples of final products analysed in 2001. The overall frequencies of Listeria spp. and L. monocytogenes in samples from all sources were 15.1% and 11.3%, respectively, but the incidence of L. monocytogenes in cold-smoked salmon final products was only 4%. A total of 201 L. monocytogenes isolates were characterised by Pulsed-Field Gel Electrophoresis (PFGE) in order to trace L. monocytogenes contamination in the processing plants. The combination of AscI and ApaI macrorestriction patterns yielded 24 different pulsotypes in 6 plants. One pulsotype observed by AscI restriction digestion comprised 148 of the 167 typed isolates from two processing plants. Two other pulsotypes predominated in samples from raw material, processing environments and final products. The results indicate that raw material, floors, and drains are potential sources of the L. monocytogenes found on cold-smoked salmon products. This highlights the need to readdress the design and cleaning of processing plants and equipment, and staff behavior. Hindering the introduction into and spread of the organism through the processing environment is necessary to avoid jeopardizing safety of the final product. |
format |
Article in Journal/Newspaper |
author |
Gudmundsdottir, Sigrun Gudbjornsdottir, Birna Lauzon, Helene L Einarsson, Hjorleifur Kristinsson, Karl G Kristjansson, Mar |
author_facet |
Gudmundsdottir, Sigrun Gudbjornsdottir, Birna Lauzon, Helene L Einarsson, Hjorleifur Kristinsson, Karl G Kristjansson, Mar |
author_sort |
Gudmundsdottir, Sigrun |
title |
Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis |
title_short |
Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis |
title_full |
Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis |
title_fullStr |
Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis |
title_full_unstemmed |
Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis |
title_sort |
tracing listeria monocytogenes isolates from cold-smoked salmon and its processing environment in iceland using pulsed-field gel electrophoresis |
publisher |
Elsevier Science Publishers |
publishDate |
2005 |
url |
http://hdl.handle.net/2336/2862 https://doi.org/10.1016/j.ijfoodmicro.2004.08.023 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://www.sciencedirect.com/science/article/B6T7K-4FDMYJ6-1/2/641c540e18e622e5cbbd11c63a4fbee9 Int. J. Food Microbiol. 2005, 101(1):41-51 0168-1605 15878405 doi:10.1016/j.ijfoodmicro.2004.08.023 http://hdl.handle.net/2336/2862 International journal of food microbiology |
op_doi |
https://doi.org/10.1016/j.ijfoodmicro.2004.08.023 |
container_title |
International Journal of Food Microbiology |
container_volume |
101 |
container_issue |
1 |
container_start_page |
41 |
op_container_end_page |
51 |
_version_ |
1766038609004068864 |