Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice.
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field. Streptococcus pneumoniae is responsible for many vaccine-preventable deaths, annually causing around 1 million deaths in children younger than 5 years of age. A new generation of pneumococ...
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Online Access: | http://hdl.handle.net/2336/237988 https://doi.org/10.1128/IAI.05801-11 |
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/237988 2023-05-15T16:52:20+02:00 Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice. Olafsdottir, Thorunn Asta Lingnau, Karen Nagy, Eszter Jonsdottir, Ingileif Landspitali, The National University Hospital of Iceland, Department of Immunology, and University of Iceland Faculty of Medicine, Reykjavik, Iceland. 2012-01 http://hdl.handle.net/2336/237988 https://doi.org/10.1128/IAI.05801-11 en eng http://dx.doi.org/10.1128/IAI.05801-11 Infect. Immun. 2012, 80(1):461-8 1098-5522 22025519 doi:10.1128/IAI.05801-11 http://hdl.handle.net/2336/237988 Infection and immunity Archived with thanks to Infection and immunity Open Access - Opinn aðgangur Adjuvants Immunologic Animals Newborn Antibodies Bacterial Bacteremia Bacterial Proteins Disease Models Animal Drug Combinations Immunization Secondary Mice Oligodeoxyribonucleotides Oligopeptides Pneumococcal Infections Pneumococcal Vaccines Streptococcus pneumoniae Th1 Cells Vaccination Article Ónæmisfræði 2012 ftlandspitaliuni https://doi.org/10.1128/IAI.05801-11 2022-05-29T08:21:49Z To access publisher full text version of this article. Please click on the hyperlink in Additional Links field. Streptococcus pneumoniae is responsible for many vaccine-preventable deaths, annually causing around 1 million deaths in children younger than 5 years of age. A new generation of pneumococcal vaccines based on conserved proteins is being developed. We evaluated the immunogenicities and protective efficacies of four pneumococcal protein vaccine candidates, PcsB, StkP, PsaA, and PspA, in a neonatal mouse model. Mice were immunized three times and challenged intranasally with virulent pneumococci. All four proteins were immunogenic in neonatal mice, and antibody (Ab) responses were significantly enhanced by the novel adjuvant IC31, which consists of an antibacterial peptide (KLKL5KLK) and a synthetic oligodeoxynucleotide, ODN1a, that signals through Toll-like receptor 9 (TLR9). Two single proteins, StkP and PspA, combined with IC31 significantly reduced pneumococcal bacteremia but had no effects on lung infection. Three proteins, PcsB, StkP, and PsaA, were evaluated with alum or IC31. IC31 enhanced Ab responses and avidity to all three proteins, whereas alum enhanced Ab responses and avidity to StkP and PsaA only. Mice receiving the trivalent protein formulation with IC31 had significantly reduced bacteremia and lung infection compared to unvaccinated mice, but the level of protection was dependent on the dose of IC31. When PspA was added to the trivalent protein formulation, the dose of IC31 needed to obtain protective immunity could be reduced. These results demonstrate that a novel pneumococcal protein-based vaccine is immunogenic at an early age of mice and emphasize the benefits of using a combination of conserved proteins and an effective adjuvant to elicit potent protective immunity against invasive pneumococcal disease. University of Iceland, Landspitali University Hospital, PATH/Intercell AG. Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive Infection and Immunity 80 1 461 468 |
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Hirsla - Landspítali University Hospital research archive |
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ftlandspitaliuni |
language |
English |
topic |
Adjuvants Immunologic Animals Newborn Antibodies Bacterial Bacteremia Bacterial Proteins Disease Models Animal Drug Combinations Immunization Secondary Mice Oligodeoxyribonucleotides Oligopeptides Pneumococcal Infections Pneumococcal Vaccines Streptococcus pneumoniae Th1 Cells Vaccination |
spellingShingle |
Adjuvants Immunologic Animals Newborn Antibodies Bacterial Bacteremia Bacterial Proteins Disease Models Animal Drug Combinations Immunization Secondary Mice Oligodeoxyribonucleotides Oligopeptides Pneumococcal Infections Pneumococcal Vaccines Streptococcus pneumoniae Th1 Cells Vaccination Olafsdottir, Thorunn Asta Lingnau, Karen Nagy, Eszter Jonsdottir, Ingileif Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice. |
topic_facet |
Adjuvants Immunologic Animals Newborn Antibodies Bacterial Bacteremia Bacterial Proteins Disease Models Animal Drug Combinations Immunization Secondary Mice Oligodeoxyribonucleotides Oligopeptides Pneumococcal Infections Pneumococcal Vaccines Streptococcus pneumoniae Th1 Cells Vaccination |
description |
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field. Streptococcus pneumoniae is responsible for many vaccine-preventable deaths, annually causing around 1 million deaths in children younger than 5 years of age. A new generation of pneumococcal vaccines based on conserved proteins is being developed. We evaluated the immunogenicities and protective efficacies of four pneumococcal protein vaccine candidates, PcsB, StkP, PsaA, and PspA, in a neonatal mouse model. Mice were immunized three times and challenged intranasally with virulent pneumococci. All four proteins were immunogenic in neonatal mice, and antibody (Ab) responses were significantly enhanced by the novel adjuvant IC31, which consists of an antibacterial peptide (KLKL5KLK) and a synthetic oligodeoxynucleotide, ODN1a, that signals through Toll-like receptor 9 (TLR9). Two single proteins, StkP and PspA, combined with IC31 significantly reduced pneumococcal bacteremia but had no effects on lung infection. Three proteins, PcsB, StkP, and PsaA, were evaluated with alum or IC31. IC31 enhanced Ab responses and avidity to all three proteins, whereas alum enhanced Ab responses and avidity to StkP and PsaA only. Mice receiving the trivalent protein formulation with IC31 had significantly reduced bacteremia and lung infection compared to unvaccinated mice, but the level of protection was dependent on the dose of IC31. When PspA was added to the trivalent protein formulation, the dose of IC31 needed to obtain protective immunity could be reduced. These results demonstrate that a novel pneumococcal protein-based vaccine is immunogenic at an early age of mice and emphasize the benefits of using a combination of conserved proteins and an effective adjuvant to elicit potent protective immunity against invasive pneumococcal disease. University of Iceland, Landspitali University Hospital, PATH/Intercell AG. |
author2 |
Landspitali, The National University Hospital of Iceland, Department of Immunology, and University of Iceland Faculty of Medicine, Reykjavik, Iceland. |
format |
Article in Journal/Newspaper |
author |
Olafsdottir, Thorunn Asta Lingnau, Karen Nagy, Eszter Jonsdottir, Ingileif |
author_facet |
Olafsdottir, Thorunn Asta Lingnau, Karen Nagy, Eszter Jonsdottir, Ingileif |
author_sort |
Olafsdottir, Thorunn Asta |
title |
Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice. |
title_short |
Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice. |
title_full |
Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice. |
title_fullStr |
Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice. |
title_full_unstemmed |
Novel protein-based pneumococcal vaccines administered with the Th1-promoting adjuvant IC31 induce protective immunity against pneumococcal disease in neonatal mice. |
title_sort |
novel protein-based pneumococcal vaccines administered with the th1-promoting adjuvant ic31 induce protective immunity against pneumococcal disease in neonatal mice. |
publishDate |
2012 |
url |
http://hdl.handle.net/2336/237988 https://doi.org/10.1128/IAI.05801-11 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://dx.doi.org/10.1128/IAI.05801-11 Infect. Immun. 2012, 80(1):461-8 1098-5522 22025519 doi:10.1128/IAI.05801-11 http://hdl.handle.net/2336/237988 Infection and immunity |
op_rights |
Archived with thanks to Infection and immunity Open Access - Opinn aðgangur |
op_doi |
https://doi.org/10.1128/IAI.05801-11 |
container_title |
Infection and Immunity |
container_volume |
80 |
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1 |
container_start_page |
461 |
op_container_end_page |
468 |
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1766042494821203968 |