Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Germ line mutations in BRCA1 and BRCA2 account for a large proportion of inherited breast and ovarian cancer. Both genes are involved in DNA repair by homologous recombination and are thoug...
Published in: | In Vitro Cellular & Developmental Biology - Animal |
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Online Access: | http://hdl.handle.net/2336/13447 https://doi.org/10.1290/0505033.1 |
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/13447 2023-05-15T16:51:49+02:00 Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation Rubner Fridriksdottir, Agla J Gudjonsson, Thorarinn Halldorsson, Thorhallur Bjornsson, Johannes Steinarsdottir, Margret Johannsson, Oskar Thor Ogmundsdottir, Helga M 2007-09-03 YES http://hdl.handle.net/2336/13447 https://doi.org/10.1290/0505033.1 en eng Society for In Vitro Biology http://proquest.umi.com/pqdweb?did=1010006671&sid=2&Fmt=2&clientId=58120&RQT=309&VName=PQD In Vitro Cell. Dev. Biol. Anim. 2005, 41(10):337-42 1071-2690 16448223 doi:10.1290/0505033.1 PTT12 MAO12 http://hdl.handle.net/2336/13447 In vitro cellular & developmental biology. Animal Breast Cell Line Tumor Chromosomal Instability Cytogenetic Analysis DNA Mutational Analysis Iceland DNA Primers Epithelial Cells Genes BRCA2 Genetic Vectors Mutation Article 2007 ftlandspitaliuni https://doi.org/10.1290/0505033.1 2022-05-29T08:21:00Z To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Germ line mutations in BRCA1 and BRCA2 account for a large proportion of inherited breast and ovarian cancer. Both genes are involved in DNA repair by homologous recombination and are thought to play a vital role in maintaining genomic stability. A major drawback for long-term functional studies of BRCA in general and BRCA2 in particular has been a lack of representative human breast epithelial cell lines. In the present study, we have established three cell lines from two patients harboring the 999del5 germ line founder mutation in the BRCA2 gene. Primary cultures were established from cellular outgrowth of explanted tissue and subsequently transfected with a retroviral construct containing the HPV-16 E6 and E7 oncogenes. Paired cancer-derived and normal-derived cell lines were established from one patient referred to as BRCA2-999del5-2T and BRCA2-999del5-2N, respectively. In addition, one cell line was derived from cancer-associated normal tissue from another patient referred to as BRCA2-999del5-1N. All three cell lines showed characteristics of breast epithelial cells as evidenced by expression of breast epithelial specific cytokeratins. Cytogenetic analysis showed marked chromosomal instability with tetraploidy and frequent telomeric associations. In conclusion, we have established three breast epithelial cell lines from two patients carrying the BRCA2 Icelandic 999del5 founder mutation. These cell lines form the basis for further studies on carcinogenesis and malignant progression of breast cancer on a defined genetic background. Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive In Vitro Cellular & Developmental Biology - Animal 41 10 337 |
institution |
Open Polar |
collection |
Hirsla - Landspítali University Hospital research archive |
op_collection_id |
ftlandspitaliuni |
language |
English |
topic |
Breast Cell Line Tumor Chromosomal Instability Cytogenetic Analysis DNA Mutational Analysis Iceland DNA Primers Epithelial Cells Genes BRCA2 Genetic Vectors Mutation |
spellingShingle |
Breast Cell Line Tumor Chromosomal Instability Cytogenetic Analysis DNA Mutational Analysis Iceland DNA Primers Epithelial Cells Genes BRCA2 Genetic Vectors Mutation Rubner Fridriksdottir, Agla J Gudjonsson, Thorarinn Halldorsson, Thorhallur Bjornsson, Johannes Steinarsdottir, Margret Johannsson, Oskar Thor Ogmundsdottir, Helga M Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation |
topic_facet |
Breast Cell Line Tumor Chromosomal Instability Cytogenetic Analysis DNA Mutational Analysis Iceland DNA Primers Epithelial Cells Genes BRCA2 Genetic Vectors Mutation |
description |
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Germ line mutations in BRCA1 and BRCA2 account for a large proportion of inherited breast and ovarian cancer. Both genes are involved in DNA repair by homologous recombination and are thought to play a vital role in maintaining genomic stability. A major drawback for long-term functional studies of BRCA in general and BRCA2 in particular has been a lack of representative human breast epithelial cell lines. In the present study, we have established three cell lines from two patients harboring the 999del5 germ line founder mutation in the BRCA2 gene. Primary cultures were established from cellular outgrowth of explanted tissue and subsequently transfected with a retroviral construct containing the HPV-16 E6 and E7 oncogenes. Paired cancer-derived and normal-derived cell lines were established from one patient referred to as BRCA2-999del5-2T and BRCA2-999del5-2N, respectively. In addition, one cell line was derived from cancer-associated normal tissue from another patient referred to as BRCA2-999del5-1N. All three cell lines showed characteristics of breast epithelial cells as evidenced by expression of breast epithelial specific cytokeratins. Cytogenetic analysis showed marked chromosomal instability with tetraploidy and frequent telomeric associations. In conclusion, we have established three breast epithelial cell lines from two patients carrying the BRCA2 Icelandic 999del5 founder mutation. These cell lines form the basis for further studies on carcinogenesis and malignant progression of breast cancer on a defined genetic background. |
format |
Article in Journal/Newspaper |
author |
Rubner Fridriksdottir, Agla J Gudjonsson, Thorarinn Halldorsson, Thorhallur Bjornsson, Johannes Steinarsdottir, Margret Johannsson, Oskar Thor Ogmundsdottir, Helga M |
author_facet |
Rubner Fridriksdottir, Agla J Gudjonsson, Thorarinn Halldorsson, Thorhallur Bjornsson, Johannes Steinarsdottir, Margret Johannsson, Oskar Thor Ogmundsdottir, Helga M |
author_sort |
Rubner Fridriksdottir, Agla J |
title |
Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation |
title_short |
Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation |
title_full |
Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation |
title_fullStr |
Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation |
title_full_unstemmed |
Establishment of three human breast epithelial cell lines derived from carriers of the 999del5 BRCA2 Icelandic founder mutation |
title_sort |
establishment of three human breast epithelial cell lines derived from carriers of the 999del5 brca2 icelandic founder mutation |
publisher |
Society for In Vitro Biology |
publishDate |
2007 |
url |
http://hdl.handle.net/2336/13447 https://doi.org/10.1290/0505033.1 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://proquest.umi.com/pqdweb?did=1010006671&sid=2&Fmt=2&clientId=58120&RQT=309&VName=PQD In Vitro Cell. Dev. Biol. Anim. 2005, 41(10):337-42 1071-2690 16448223 doi:10.1290/0505033.1 PTT12 MAO12 http://hdl.handle.net/2336/13447 In vitro cellular & developmental biology. Animal |
op_doi |
https://doi.org/10.1290/0505033.1 |
container_title |
In Vitro Cellular & Developmental Biology - Animal |
container_volume |
41 |
container_issue |
10 |
container_start_page |
337 |
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1766041910573531136 |