Broiler Campylobacter contamination and human campylobacteriosis in Iceland
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field To examine whether there is a relationship between the degree of Campylobacter contamination observed in product lots of retail Icelandic broiler chicken carcasses and the incidence of huma...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , , , , , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2011
|
Subjects: | |
Online Access: | http://hdl.handle.net/2336/125811 https://doi.org/10.1128/AEM.01129-08 |
id |
ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/125811 |
---|---|
record_format |
openpolar |
spelling |
ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/125811 2023-05-15T16:49:08+02:00 Broiler Campylobacter contamination and human campylobacteriosis in Iceland Callicott, Kenneth A Hardardottir, Hjordis Georgsson, Franklin Reiersen, Jarle Fridriksdottir, Vala Gunnarsson, Eggert Michel, Pascal Bisaillon, Jean-Robert Kristinsson, Karl G Briem, Haraldur Hiett, Kelli L Needleman, David S Stern, Norman J Poultry Microbiological Safety Research Unit, USDA-ARS, Athens, GA 30605, USA. 2011-03-28 http://hdl.handle.net/2336/125811 https://doi.org/10.1128/AEM.01129-08 en eng American Society for Microbiology http://dx.doi.org/10.1128/AEM.01129-08 Appl. Environ. Microbiol. 2008, 74(21):6483-94 1098-5336 18791017 doi:10.1128/AEM.01129-08 http://hdl.handle.net/2336/125811 Applied and environmental microbiology Animals Bacterial Typing Techniques Campylobacter Campylobacter Infections Chickens Cluster Analysis Colony Count Microbial DNA Bacterial Flagellin Food Contamination Genotype Humans Iceland Incidence Meat Molecular Epidemiology Molecular Sequence Data Sequence Analysis Article 2011 ftlandspitaliuni https://doi.org/10.1128/AEM.01129-08 2022-05-29T08:21:43Z To access publisher full text version of this article. Please click on the hyperlink in Additional Links field To examine whether there is a relationship between the degree of Campylobacter contamination observed in product lots of retail Icelandic broiler chicken carcasses and the incidence of human disease, 1,617 isolates from 327 individual product lots were genetically matched (using the flaA short variable region [SVR[) to 289 isolates from cases of human campylobacteriosis whose onset was within approximately 2 weeks from the date of processing. When there was genetic identity between broiler isolates and human isolates within the appropriate time frame, a retail product lot was classified as implicated in human disease. According to the results of this analysis, there were multiple clusters of human disease linked to the same process lot or lots. Implicated and nonimplicated retail product lots were compared for four lot descriptors: lot size, prevalence, mean contamination, and maximum contamination (as characterized by direct rinse plating). For retail product distributed fresh, Mann-Whitney U tests showed that implicated product lots had significantly (P = 0.0055) higher mean contamination than nonimplicated lots. The corresponding median values were 3.56 log CFU/carcass for implicated lots and 2.72 log CFU/carcass for nonimplicated lots. For frozen retail product, implicated lots were significantly (P = 0.0281) larger than nonimplicated lots. When the time frame was removed, retail product lots containing Campylobacter flaA SVR genotypes also seen in human disease had significantly higher mean and maximum contamination numbers than lots containing no genotypes seen in human disease for both fresh and frozen product. Our results suggest that cases of broiler-borne campylobacteriosis may occur in clusters and that the differences in mean contamination levels may provide a basis for regulatory action that is more specific than a presence-absence standard. Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive Applied and Environmental Microbiology 74 21 6483 6494 |
institution |
Open Polar |
collection |
Hirsla - Landspítali University Hospital research archive |
op_collection_id |
ftlandspitaliuni |
language |
English |
topic |
Animals Bacterial Typing Techniques Campylobacter Campylobacter Infections Chickens Cluster Analysis Colony Count Microbial DNA Bacterial Flagellin Food Contamination Genotype Humans Iceland Incidence Meat Molecular Epidemiology Molecular Sequence Data Sequence Analysis |
spellingShingle |
Animals Bacterial Typing Techniques Campylobacter Campylobacter Infections Chickens Cluster Analysis Colony Count Microbial DNA Bacterial Flagellin Food Contamination Genotype Humans Iceland Incidence Meat Molecular Epidemiology Molecular Sequence Data Sequence Analysis Callicott, Kenneth A Hardardottir, Hjordis Georgsson, Franklin Reiersen, Jarle Fridriksdottir, Vala Gunnarsson, Eggert Michel, Pascal Bisaillon, Jean-Robert Kristinsson, Karl G Briem, Haraldur Hiett, Kelli L Needleman, David S Stern, Norman J Broiler Campylobacter contamination and human campylobacteriosis in Iceland |
topic_facet |
Animals Bacterial Typing Techniques Campylobacter Campylobacter Infections Chickens Cluster Analysis Colony Count Microbial DNA Bacterial Flagellin Food Contamination Genotype Humans Iceland Incidence Meat Molecular Epidemiology Molecular Sequence Data Sequence Analysis |
description |
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field To examine whether there is a relationship between the degree of Campylobacter contamination observed in product lots of retail Icelandic broiler chicken carcasses and the incidence of human disease, 1,617 isolates from 327 individual product lots were genetically matched (using the flaA short variable region [SVR[) to 289 isolates from cases of human campylobacteriosis whose onset was within approximately 2 weeks from the date of processing. When there was genetic identity between broiler isolates and human isolates within the appropriate time frame, a retail product lot was classified as implicated in human disease. According to the results of this analysis, there were multiple clusters of human disease linked to the same process lot or lots. Implicated and nonimplicated retail product lots were compared for four lot descriptors: lot size, prevalence, mean contamination, and maximum contamination (as characterized by direct rinse plating). For retail product distributed fresh, Mann-Whitney U tests showed that implicated product lots had significantly (P = 0.0055) higher mean contamination than nonimplicated lots. The corresponding median values were 3.56 log CFU/carcass for implicated lots and 2.72 log CFU/carcass for nonimplicated lots. For frozen retail product, implicated lots were significantly (P = 0.0281) larger than nonimplicated lots. When the time frame was removed, retail product lots containing Campylobacter flaA SVR genotypes also seen in human disease had significantly higher mean and maximum contamination numbers than lots containing no genotypes seen in human disease for both fresh and frozen product. Our results suggest that cases of broiler-borne campylobacteriosis may occur in clusters and that the differences in mean contamination levels may provide a basis for regulatory action that is more specific than a presence-absence standard. |
author2 |
Poultry Microbiological Safety Research Unit, USDA-ARS, Athens, GA 30605, USA. |
format |
Article in Journal/Newspaper |
author |
Callicott, Kenneth A Hardardottir, Hjordis Georgsson, Franklin Reiersen, Jarle Fridriksdottir, Vala Gunnarsson, Eggert Michel, Pascal Bisaillon, Jean-Robert Kristinsson, Karl G Briem, Haraldur Hiett, Kelli L Needleman, David S Stern, Norman J |
author_facet |
Callicott, Kenneth A Hardardottir, Hjordis Georgsson, Franklin Reiersen, Jarle Fridriksdottir, Vala Gunnarsson, Eggert Michel, Pascal Bisaillon, Jean-Robert Kristinsson, Karl G Briem, Haraldur Hiett, Kelli L Needleman, David S Stern, Norman J |
author_sort |
Callicott, Kenneth A |
title |
Broiler Campylobacter contamination and human campylobacteriosis in Iceland |
title_short |
Broiler Campylobacter contamination and human campylobacteriosis in Iceland |
title_full |
Broiler Campylobacter contamination and human campylobacteriosis in Iceland |
title_fullStr |
Broiler Campylobacter contamination and human campylobacteriosis in Iceland |
title_full_unstemmed |
Broiler Campylobacter contamination and human campylobacteriosis in Iceland |
title_sort |
broiler campylobacter contamination and human campylobacteriosis in iceland |
publisher |
American Society for Microbiology |
publishDate |
2011 |
url |
http://hdl.handle.net/2336/125811 https://doi.org/10.1128/AEM.01129-08 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://dx.doi.org/10.1128/AEM.01129-08 Appl. Environ. Microbiol. 2008, 74(21):6483-94 1098-5336 18791017 doi:10.1128/AEM.01129-08 http://hdl.handle.net/2336/125811 Applied and environmental microbiology |
op_doi |
https://doi.org/10.1128/AEM.01129-08 |
container_title |
Applied and Environmental Microbiology |
container_volume |
74 |
container_issue |
21 |
container_start_page |
6483 |
op_container_end_page |
6494 |
_version_ |
1766039208682586112 |