Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field This study was based on 358 cases with abnormal smears referred for colposcopy and HPV DNA testing. We analysed: 1) the frequency of different grades of cyto- and histopathologic findings;...
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/111313 2023-05-15T16:53:01+02:00 Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears Sigurdsson, K Arnadottir, T Snorradottir, M Benediktsdottir, K Saemundsson, H The Icelandic Cancer Society, The Cancer Detection Clinic, Reykjavik. krabb@krabb.is 2010-09-17 http://hdl.handle.net/2336/111313 en eng John Wiley & Sons Inc http://dx.doi.org/10.1002/(SICI)1097-0215(19970729)72:3<446::AID-IJC12>3.0.CO;2-A Int. J. Cancer. 1997, 72(3):446-52 0020-7136 9247288 http://hdl.handle.net/2336/111313 International journal of cancer. Journal international du cancer Biopsy Cervical Intraepithelial Neoplasia Colposcopy DNA Viral Female Humans Iceland Nucleic Acid Hybridization Papillomaviridae Polymerase Chain Reaction Vaginal Smears Article 2010 ftlandspitaliuni https://doi.org/10.1002/(SICI)1097-0215(19970729)72:3<446::AID-IJC12>3.0.CO;2-A 2022-05-29T08:21:37Z To access publisher full text version of this article. Please click on the hyperlink in Additional Links field This study was based on 358 cases with abnormal smears referred for colposcopy and HPV DNA testing. We analysed: 1) the frequency of different grades of cyto- and histopathologic findings; 2) the frequency and relative amount of HPV DNA with the hybrid capture assay (HCA) in swabs, and the frequency of HPV with PCR in swabs (-S) and biopsies (-B); and 3) the frequency of HPV types according to the grade of the cyto- and histopathologic findings. Of all cases, 95% were positive with all HPV tests combined. The HCA (HPV: 16, 18, 31, 33, 35, 45, 51, 52 and 56) and the PCR-S and PCR-B (HPV: 16, 18, 31, 33 and 35) tests for high-risk HPV exhibited sensitivities of 57%, 56% and 48%, respectively. The high-grade smears and the high-risk PCR-S HPV had about 80% sensitivity for histologic high-grade lesions compared with around 70% for HCA and the PCR-B. Combining the high-grade smears and the high-risk HPV increased the sensitivity to 93-96%. Among the cervical intraepithelial neoplasia I (CIN I) and the atypical squamous cells of undetermined significance (ASCUS) smears the sensitivity of high-risk HPV for high-grade histologic lesions was 63% for the HCA and 79% for the PCR-S. No correlation was found between the relative amount of HPV DNA detected by HCA and the grade of cyto- and histological lesions. We conclude that the results strongly indicate that HCA is less sensitive than PCR in the diagnosis of high-risk HPV, that swabs are more sensitive than biopsies as a sampling method, that high-risk HPV and high-grade smears are complementary for the diagnosis of high-grade histologic lesions and that the present role of HPV testing in screening could be limited to identifying women with low-grade smears and koilocytotic or low-grade colposcopic biopsies that are at risk of concealing or developing high-grade histologic lesions. Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive |
institution |
Open Polar |
collection |
Hirsla - Landspítali University Hospital research archive |
op_collection_id |
ftlandspitaliuni |
language |
English |
topic |
Biopsy Cervical Intraepithelial Neoplasia Colposcopy DNA Viral Female Humans Iceland Nucleic Acid Hybridization Papillomaviridae Polymerase Chain Reaction Vaginal Smears |
spellingShingle |
Biopsy Cervical Intraepithelial Neoplasia Colposcopy DNA Viral Female Humans Iceland Nucleic Acid Hybridization Papillomaviridae Polymerase Chain Reaction Vaginal Smears Sigurdsson, K Arnadottir, T Snorradottir, M Benediktsdottir, K Saemundsson, H Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears |
topic_facet |
Biopsy Cervical Intraepithelial Neoplasia Colposcopy DNA Viral Female Humans Iceland Nucleic Acid Hybridization Papillomaviridae Polymerase Chain Reaction Vaginal Smears |
description |
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field This study was based on 358 cases with abnormal smears referred for colposcopy and HPV DNA testing. We analysed: 1) the frequency of different grades of cyto- and histopathologic findings; 2) the frequency and relative amount of HPV DNA with the hybrid capture assay (HCA) in swabs, and the frequency of HPV with PCR in swabs (-S) and biopsies (-B); and 3) the frequency of HPV types according to the grade of the cyto- and histopathologic findings. Of all cases, 95% were positive with all HPV tests combined. The HCA (HPV: 16, 18, 31, 33, 35, 45, 51, 52 and 56) and the PCR-S and PCR-B (HPV: 16, 18, 31, 33 and 35) tests for high-risk HPV exhibited sensitivities of 57%, 56% and 48%, respectively. The high-grade smears and the high-risk PCR-S HPV had about 80% sensitivity for histologic high-grade lesions compared with around 70% for HCA and the PCR-B. Combining the high-grade smears and the high-risk HPV increased the sensitivity to 93-96%. Among the cervical intraepithelial neoplasia I (CIN I) and the atypical squamous cells of undetermined significance (ASCUS) smears the sensitivity of high-risk HPV for high-grade histologic lesions was 63% for the HCA and 79% for the PCR-S. No correlation was found between the relative amount of HPV DNA detected by HCA and the grade of cyto- and histological lesions. We conclude that the results strongly indicate that HCA is less sensitive than PCR in the diagnosis of high-risk HPV, that swabs are more sensitive than biopsies as a sampling method, that high-risk HPV and high-grade smears are complementary for the diagnosis of high-grade histologic lesions and that the present role of HPV testing in screening could be limited to identifying women with low-grade smears and koilocytotic or low-grade colposcopic biopsies that are at risk of concealing or developing high-grade histologic lesions. |
author2 |
The Icelandic Cancer Society, The Cancer Detection Clinic, Reykjavik. krabb@krabb.is |
format |
Article in Journal/Newspaper |
author |
Sigurdsson, K Arnadottir, T Snorradottir, M Benediktsdottir, K Saemundsson, H |
author_facet |
Sigurdsson, K Arnadottir, T Snorradottir, M Benediktsdottir, K Saemundsson, H |
author_sort |
Sigurdsson, K |
title |
Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears |
title_short |
Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears |
title_full |
Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears |
title_fullStr |
Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears |
title_full_unstemmed |
Human papillomavirus (HPV) in an Icelandic population: the role of HPV DNA testing based on hybrid capture and PCR assays among women with screen-detected abnormal Pap smears |
title_sort |
human papillomavirus (hpv) in an icelandic population: the role of hpv dna testing based on hybrid capture and pcr assays among women with screen-detected abnormal pap smears |
publisher |
John Wiley & Sons Inc |
publishDate |
2010 |
url |
http://hdl.handle.net/2336/111313 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://dx.doi.org/10.1002/(SICI)1097-0215(19970729)72:3<446::AID-IJC12>3.0.CO;2-A Int. J. Cancer. 1997, 72(3):446-52 0020-7136 9247288 http://hdl.handle.net/2336/111313 International journal of cancer. Journal international du cancer |
op_doi |
https://doi.org/10.1002/(SICI)1097-0215(19970729)72:3<446::AID-IJC12>3.0.CO;2-A |
_version_ |
1766043523731161088 |