Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Since their first detection in 1988, penicillin-resistant Streptococcus pneumoniae isolates have rapidly spread in Iceland to account for close to 20% of all pneumococcal disease in that co...
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/110135 2023-05-15T16:47:14+02:00 Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits Vilhelmsson, S E Tomasz, A Kristinsson, K G The Rockefeller University, New York, NY, USA. 2010-07-23 http://hdl.handle.net/2336/110135 en eng American Society for Microbiology http://jcm.asm.org/cgi/content/abstract/38/4/1375 J. Clin. Microbiol. 2000, 38(4):1375-81 0095-1137 10747110 http://hdl.handle.net/2336/110135 Journal of clinical microbiology Adult Aged 80 and over Anti-Bacterial Agents Child Preschool Drug Resistance Microbial Multiple Electrophoresis Gel Pulsed-Field Evolution Molecular Humans Iceland Infant Microbial Sensitivity Tests Middle Aged Nucleic Acid Hybridization Penicillin Resistance Pneumococcal Infections Serotyping Spain Streptococcus pneumoniae United States Article 2010 ftlandspitaliuni 2022-05-29T08:21:35Z To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Since their first detection in 1988, penicillin-resistant Streptococcus pneumoniae isolates have rapidly spread in Iceland to account for close to 20% of all pneumococcal disease in that country by 1993. The major component (70%) of the resistant pneumococci identified from 1989 to 1992 was the progeny of a single multidrug-resistant clone (Icelandic clone) with a homogeneous chromosomal macrorestriction profile and identical multilocus enzyme type expressing serotype 6B and resistance to penicillin, tetracycline, chloramphenicol, erythromycin, and trimethoprim-sulfamethoxazole. The rest of the non-penicillin-susceptible isolates included bacteria with serotype 6A and serogroups 19 and 23. The unique geographic and epidemiological setting and the availability of a complete collection of all non-penicillin-susceptible isolates of S. pneumoniae in Iceland prompted us to carry out a molecular epidemiological study to monitor the fate of the Icelandic clone between 1989 and 1996; in addition, we wished to extend the characterization to representative groups of all non-penicillin-susceptible serotype 6B pneumococci which showed variations in antibiotype and which were recovered in Iceland between late 1989 and the end of 1996. Also included in the study were non-penicillin-susceptible isolates of serogroup 23. Pulsed-field gel electrophoresis of SmaI-restricted chromosomal DNA and Southern hybridization with the lytA DNA probe and probes specific for antibiotic resistance genes were used to characterize pneumococcal isolates. The results show that (i) the Icelandic clone remained the predominant type among penicillin-resistant S. pneumoniae through 1996; (ii) the emergence of variants of the Icelandic clone which had lost one or more of the antibiotic resistance phenotypes and/or resistant genes, singly or in combination, was documented during the surveillance period; and (iii) isolates belonging to the ... Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive |
institution |
Open Polar |
collection |
Hirsla - Landspítali University Hospital research archive |
op_collection_id |
ftlandspitaliuni |
language |
English |
topic |
Adult Aged 80 and over Anti-Bacterial Agents Child Preschool Drug Resistance Microbial Multiple Electrophoresis Gel Pulsed-Field Evolution Molecular Humans Iceland Infant Microbial Sensitivity Tests Middle Aged Nucleic Acid Hybridization Penicillin Resistance Pneumococcal Infections Serotyping Spain Streptococcus pneumoniae United States |
spellingShingle |
Adult Aged 80 and over Anti-Bacterial Agents Child Preschool Drug Resistance Microbial Multiple Electrophoresis Gel Pulsed-Field Evolution Molecular Humans Iceland Infant Microbial Sensitivity Tests Middle Aged Nucleic Acid Hybridization Penicillin Resistance Pneumococcal Infections Serotyping Spain Streptococcus pneumoniae United States Vilhelmsson, S E Tomasz, A Kristinsson, K G Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits |
topic_facet |
Adult Aged 80 and over Anti-Bacterial Agents Child Preschool Drug Resistance Microbial Multiple Electrophoresis Gel Pulsed-Field Evolution Molecular Humans Iceland Infant Microbial Sensitivity Tests Middle Aged Nucleic Acid Hybridization Penicillin Resistance Pneumococcal Infections Serotyping Spain Streptococcus pneumoniae United States |
description |
To access publisher full text version of this article. Please click on the hyperlink in Additional Links field Since their first detection in 1988, penicillin-resistant Streptococcus pneumoniae isolates have rapidly spread in Iceland to account for close to 20% of all pneumococcal disease in that country by 1993. The major component (70%) of the resistant pneumococci identified from 1989 to 1992 was the progeny of a single multidrug-resistant clone (Icelandic clone) with a homogeneous chromosomal macrorestriction profile and identical multilocus enzyme type expressing serotype 6B and resistance to penicillin, tetracycline, chloramphenicol, erythromycin, and trimethoprim-sulfamethoxazole. The rest of the non-penicillin-susceptible isolates included bacteria with serotype 6A and serogroups 19 and 23. The unique geographic and epidemiological setting and the availability of a complete collection of all non-penicillin-susceptible isolates of S. pneumoniae in Iceland prompted us to carry out a molecular epidemiological study to monitor the fate of the Icelandic clone between 1989 and 1996; in addition, we wished to extend the characterization to representative groups of all non-penicillin-susceptible serotype 6B pneumococci which showed variations in antibiotype and which were recovered in Iceland between late 1989 and the end of 1996. Also included in the study were non-penicillin-susceptible isolates of serogroup 23. Pulsed-field gel electrophoresis of SmaI-restricted chromosomal DNA and Southern hybridization with the lytA DNA probe and probes specific for antibiotic resistance genes were used to characterize pneumococcal isolates. The results show that (i) the Icelandic clone remained the predominant type among penicillin-resistant S. pneumoniae through 1996; (ii) the emergence of variants of the Icelandic clone which had lost one or more of the antibiotic resistance phenotypes and/or resistant genes, singly or in combination, was documented during the surveillance period; and (iii) isolates belonging to the ... |
author2 |
The Rockefeller University, New York, NY, USA. |
format |
Article in Journal/Newspaper |
author |
Vilhelmsson, S E Tomasz, A Kristinsson, K G |
author_facet |
Vilhelmsson, S E Tomasz, A Kristinsson, K G |
author_sort |
Vilhelmsson, S E |
title |
Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits |
title_short |
Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits |
title_full |
Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits |
title_fullStr |
Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits |
title_full_unstemmed |
Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits |
title_sort |
molecular evolution in a multidrug-resistant lineage of streptococcus pneumoniae: emergence of strains belonging to the serotype 6b icelandic clone that lost antibiotic resistance traits |
publisher |
American Society for Microbiology |
publishDate |
2010 |
url |
http://hdl.handle.net/2336/110135 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://jcm.asm.org/cgi/content/abstract/38/4/1375 J. Clin. Microbiol. 2000, 38(4):1375-81 0095-1137 10747110 http://hdl.handle.net/2336/110135 Journal of clinical microbiology |
_version_ |
1766037313576501248 |