Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery

Antarctic have been suggested as an attractive source for antibiotics discovery and members of Streptomyces genus have historically been studied as natural producers of antimicrobial metabolites. Nonetheless, our knowledge on antibiotic-producing Streptomyces from Antarctic is very limited. In this...

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Main Authors: Núñez-Montero, Kattia, Lamilla, Claudio, Abanto, Michel, Maruyama, Fumito, Jorquera, Milko A., Santos, Andrés, Martinez-Urtaza, Jaime, Barrientos, Leticia
Other Authors: 丸山, 史人
Format: Article in Journal/Newspaper
Language:English
Published: Springer Nature 2019
Subjects:
Online Access:http://hdl.handle.net/2433/250003
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spelling ftkyotouniv:oai:repository.kulib.kyoto-u.ac.jp:2433/250003 2023-05-15T13:41:42+02:00 Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery Núñez-Montero, Kattia Lamilla, Claudio Abanto, Michel Maruyama, Fumito Jorquera, Milko A. Santos, Andrés Martinez-Urtaza, Jaime Barrientos, Leticia 丸山, 史人 2019-05-16 application/pdf http://hdl.handle.net/2433/250003 eng eng Springer Nature 10.1038/s41598-019-43960-7 2045-2322 http://hdl.handle.net/2433/250003 Scientific Reports 9 7488 31097761 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Antibiotics Applied microbiology Bacterial genes Bacteriology Genome evolution Journal Article 2019 ftkyotouniv 2020-04-02T23:13:50Z Antarctic have been suggested as an attractive source for antibiotics discovery and members of Streptomyces genus have historically been studied as natural producers of antimicrobial metabolites. Nonetheless, our knowledge on antibiotic-producing Streptomyces from Antarctic is very limited. In this study, the antimicrobial activity of organic extracts from Antarctic Streptomyces strains was evaluated by disk diffusion assays and minimum inhibitory concentration. The strain Streptomyces sp. So13.3 showed the greatest antibiotic activity (MIC = 15.6 μg/mL) against Gram-positive bacteria and growth reduction of Gram‒negative pathogens. The bioactive fraction in the crude extract was revealed by TLC‒bioautography at Rf = 0.78 with molecular weight between 148 and 624 m/z detected by LC-ESI-MS/MS. The strain So13.3 was taxonomically affiliated as Streptomyces fildesensis. Whole genome sequencing and analysis suggested a 9.47 Mb genome size with 42 predicted biosynthetic gene clusters (BGCs) and 56 putative clusters representing a 22% of total genome content. Interestingly, a large number of them (11 of 42 BGCs and 40 of 56 putative BGCs), did not show similarities with other known BGCs. Our results highlight the potential of the Antarctic Streptomyces strains as a promising source of novel antimicrobials, particularly the strain Streptomyces fildesensis So13.3, which first draft genome is reported in this work. Article in Journal/Newspaper Antarc* Antarctic Kyoto University Research Information Repository (KURENAI) Antarctic The Antarctic
institution Open Polar
collection Kyoto University Research Information Repository (KURENAI)
op_collection_id ftkyotouniv
language English
topic Antibiotics
Applied microbiology
Bacterial genes
Bacteriology
Genome evolution
spellingShingle Antibiotics
Applied microbiology
Bacterial genes
Bacteriology
Genome evolution
Núñez-Montero, Kattia
Lamilla, Claudio
Abanto, Michel
Maruyama, Fumito
Jorquera, Milko A.
Santos, Andrés
Martinez-Urtaza, Jaime
Barrientos, Leticia
Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
topic_facet Antibiotics
Applied microbiology
Bacterial genes
Bacteriology
Genome evolution
description Antarctic have been suggested as an attractive source for antibiotics discovery and members of Streptomyces genus have historically been studied as natural producers of antimicrobial metabolites. Nonetheless, our knowledge on antibiotic-producing Streptomyces from Antarctic is very limited. In this study, the antimicrobial activity of organic extracts from Antarctic Streptomyces strains was evaluated by disk diffusion assays and minimum inhibitory concentration. The strain Streptomyces sp. So13.3 showed the greatest antibiotic activity (MIC = 15.6 μg/mL) against Gram-positive bacteria and growth reduction of Gram‒negative pathogens. The bioactive fraction in the crude extract was revealed by TLC‒bioautography at Rf = 0.78 with molecular weight between 148 and 624 m/z detected by LC-ESI-MS/MS. The strain So13.3 was taxonomically affiliated as Streptomyces fildesensis. Whole genome sequencing and analysis suggested a 9.47 Mb genome size with 42 predicted biosynthetic gene clusters (BGCs) and 56 putative clusters representing a 22% of total genome content. Interestingly, a large number of them (11 of 42 BGCs and 40 of 56 putative BGCs), did not show similarities with other known BGCs. Our results highlight the potential of the Antarctic Streptomyces strains as a promising source of novel antimicrobials, particularly the strain Streptomyces fildesensis So13.3, which first draft genome is reported in this work.
author2 丸山, 史人
format Article in Journal/Newspaper
author Núñez-Montero, Kattia
Lamilla, Claudio
Abanto, Michel
Maruyama, Fumito
Jorquera, Milko A.
Santos, Andrés
Martinez-Urtaza, Jaime
Barrientos, Leticia
author_facet Núñez-Montero, Kattia
Lamilla, Claudio
Abanto, Michel
Maruyama, Fumito
Jorquera, Milko A.
Santos, Andrés
Martinez-Urtaza, Jaime
Barrientos, Leticia
author_sort Núñez-Montero, Kattia
title Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_short Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_full Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_fullStr Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_full_unstemmed Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery
title_sort antarctic streptomyces fildesensis so13.3 strain as a promising source for antimicrobials discovery
publisher Springer Nature
publishDate 2019
url http://hdl.handle.net/2433/250003
geographic Antarctic
The Antarctic
geographic_facet Antarctic
The Antarctic
genre Antarc*
Antarctic
genre_facet Antarc*
Antarctic
op_relation 10.1038/s41598-019-43960-7
2045-2322
http://hdl.handle.net/2433/250003
Scientific Reports
9
7488
31097761
op_rights This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
op_rightsnorm CC-BY
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