Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy
Optimization of lipase-catalyzed production of industrially important isoamyl acetate was carried out by response surface methodology (RSM). Isoamyl alcohol which was distillated from fusel oil and acetic acid were used as the substrates. Immobilized lipase from Candida antarctica (Novozym 435) was...
Published in: | Enzyme and Microbial Technology |
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2007
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ftkocaeliuniv:faf0e616-6120-4095-a280-ea5e1862ade8 2023-05-15T13:43:59+02:00 Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy Kapucu, NURCAN MEHMETOĞLU, ÜLKÜ GÜVENÇ, AFİFE KAPUCU, HAMDİ AYDOĞAN, ÖZLEM 2007-03-05T01:00:00Z https://doi.org/10.1016/j.enzmictec.2006.06.010 https://avesis.kocaeli.edu.tr/publication/details/faf0e616-6120-4095-a280-ea5e1862ade8/oai eng eng faf0e616-6120-4095-a280-ea5e1862ade8 doi:10.1016/j.enzmictec.2006.06.010 https://avesis.kocaeli.edu.tr/publication/details/faf0e616-6120-4095-a280-ea5e1862ade8/oai info:eu-repo/semantics/closedAccess info:eu-repo/semantics/article 2007 ftkocaeliuniv https://doi.org/10.1016/j.enzmictec.2006.06.010 2022-11-17T10:23:02Z Optimization of lipase-catalyzed production of industrially important isoamyl acetate was carried out by response surface methodology (RSM). Isoamyl alcohol which was distillated from fusel oil and acetic acid were used as the substrates. Immobilized lipase from Candida antarctica (Novozym 435) was catalyzed to this reaction in the solvent-free system. RSM based on five-level, four variable central composite rotatable design was used to evaluate the effects of important parameters on the production of isoamyl acetate. The parameters are acid/alcohol mole ratio (0.2-0.8), enzyme amount (4-12%, w/w), temperature (30-50 degrees C) and reaction time (4-8 h). It was found that the most effective parameter was acid/alcohol mole ratio. The model indicated the optimum conditions for maximum esterification (4.4 mmol ester/g mixture) in the acid/alcohol mole ratio of 0.8 for 12% (w/w) enzyme at 30 degrees C and after 8 h, which were in good agreement with the experimental value. The coefficient of determination (R-2) for the model is 0.9968. Probability value (P < 0.0001) demonstrates a very high significance for the regression model. (c) 2006 Elsevier Inc. All rights reserved. Article in Journal/Newspaper Antarc* Antarctica Kocaeli University Research Information System Enzyme and Microbial Technology 40 4 778 785 |
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English |
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Optimization of lipase-catalyzed production of industrially important isoamyl acetate was carried out by response surface methodology (RSM). Isoamyl alcohol which was distillated from fusel oil and acetic acid were used as the substrates. Immobilized lipase from Candida antarctica (Novozym 435) was catalyzed to this reaction in the solvent-free system. RSM based on five-level, four variable central composite rotatable design was used to evaluate the effects of important parameters on the production of isoamyl acetate. The parameters are acid/alcohol mole ratio (0.2-0.8), enzyme amount (4-12%, w/w), temperature (30-50 degrees C) and reaction time (4-8 h). It was found that the most effective parameter was acid/alcohol mole ratio. The model indicated the optimum conditions for maximum esterification (4.4 mmol ester/g mixture) in the acid/alcohol mole ratio of 0.8 for 12% (w/w) enzyme at 30 degrees C and after 8 h, which were in good agreement with the experimental value. The coefficient of determination (R-2) for the model is 0.9968. Probability value (P < 0.0001) demonstrates a very high significance for the regression model. (c) 2006 Elsevier Inc. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Kapucu, NURCAN MEHMETOĞLU, ÜLKÜ GÜVENÇ, AFİFE KAPUCU, HAMDİ AYDOĞAN, ÖZLEM |
spellingShingle |
Kapucu, NURCAN MEHMETOĞLU, ÜLKÜ GÜVENÇ, AFİFE KAPUCU, HAMDİ AYDOĞAN, ÖZLEM Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy |
author_facet |
Kapucu, NURCAN MEHMETOĞLU, ÜLKÜ GÜVENÇ, AFİFE KAPUCU, HAMDİ AYDOĞAN, ÖZLEM |
author_sort |
Kapucu, NURCAN |
title |
Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy |
title_short |
Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy |
title_full |
Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy |
title_fullStr |
Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy |
title_full_unstemmed |
Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy |
title_sort |
enzymatic esterification of isoamyl alcohol obtained from fusel oil: optimization by response surface methodolgy |
publishDate |
2007 |
url |
https://doi.org/10.1016/j.enzmictec.2006.06.010 https://avesis.kocaeli.edu.tr/publication/details/faf0e616-6120-4095-a280-ea5e1862ade8/oai |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
faf0e616-6120-4095-a280-ea5e1862ade8 doi:10.1016/j.enzmictec.2006.06.010 https://avesis.kocaeli.edu.tr/publication/details/faf0e616-6120-4095-a280-ea5e1862ade8/oai |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.enzmictec.2006.06.010 |
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Enzyme and Microbial Technology |
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40 |
container_issue |
4 |
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778 |
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785 |
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1766195687175749632 |