Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology
Lipase catalyzed production of oleyl oleate, which is an analogue of jojoba oil, was carried out using oleic acid and oleyl alcohol in the solvent-free system. Novozym 435, immobilized Candida antarctica lipase, was used as a biocatalyst. Response surface methodology (RSM) based on five-level, four-...
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ftkocaeliuniv:58a7f52e-de0d-4f57-937c-b314ff1acbd6 2023-05-15T14:01:41+02:00 Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology MEHMETOĞLU, ÜLKÜ KAPUCU, HAMDİ ÇALIMLI, AYLA GÜVENÇ, AFİFE Kapucu, NURCAN 2003-05-01T00:00:00Z https://doi.org/10.1080/00986440390207396 https://avesis.kocaeli.edu.tr/publication/details/58a7f52e-de0d-4f57-937c-b314ff1acbd6/oai eng eng 58a7f52e-de0d-4f57-937c-b314ff1acbd6 doi:10.1080/00986440390207396 https://avesis.kocaeli.edu.tr/publication/details/58a7f52e-de0d-4f57-937c-b314ff1acbd6/oai info:eu-repo/semantics/closedAccess info:eu-repo/semantics/article 2003 ftkocaeliuniv https://doi.org/10.1080/00986440390207396 2022-11-17T10:08:09Z Lipase catalyzed production of oleyl oleate, which is an analogue of jojoba oil, was carried out using oleic acid and oleyl alcohol in the solvent-free system. Novozym 435, immobilized Candida antarctica lipase, was used as a biocatalyst. Response surface methodology (RSM) based on five-level, four-variable central composite rotatable design was used to evaluate the effects of important parameters on the production of oleyl oleate. Acid/alcohol molar ratio (0.5-1.5), enzyme quantity (2-10% w/w of substrates), reaction temperature (40-60degreesC), and reaction time (30-90 min) were chosen as process variables for the optimization. Among these parameters, enzyme quantity and acid/alcohol molar ratio have significant effects compared with temperature and time on the production of oleyl oleate. Optimum conditions were found to be a acid/alcohol molar ratio of 1, enzyme quantity of 7% (w/w), reaction temperature of 51degreesC, and reaction time of 75 min. The coefficient of determination (R-2 ) for the model is 0.97. Probability value is 2.9x10(-9) (P-value<0.01). This P-value demonstrates a very high significance for the regression model. The maximum oleyl oleate concentration predicted by the equation (737 g/L) agrees well with the experimentalvalue (734 g/L) obtained from the experimental verification at the optimum values. Article in Journal/Newspaper Antarc* Antarctica Kocaeli University Research Information System |
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Open Polar |
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Kocaeli University Research Information System |
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language |
English |
description |
Lipase catalyzed production of oleyl oleate, which is an analogue of jojoba oil, was carried out using oleic acid and oleyl alcohol in the solvent-free system. Novozym 435, immobilized Candida antarctica lipase, was used as a biocatalyst. Response surface methodology (RSM) based on five-level, four-variable central composite rotatable design was used to evaluate the effects of important parameters on the production of oleyl oleate. Acid/alcohol molar ratio (0.5-1.5), enzyme quantity (2-10% w/w of substrates), reaction temperature (40-60degreesC), and reaction time (30-90 min) were chosen as process variables for the optimization. Among these parameters, enzyme quantity and acid/alcohol molar ratio have significant effects compared with temperature and time on the production of oleyl oleate. Optimum conditions were found to be a acid/alcohol molar ratio of 1, enzyme quantity of 7% (w/w), reaction temperature of 51degreesC, and reaction time of 75 min. The coefficient of determination (R-2 ) for the model is 0.97. Probability value is 2.9x10(-9) (P-value<0.01). This P-value demonstrates a very high significance for the regression model. The maximum oleyl oleate concentration predicted by the equation (737 g/L) agrees well with the experimentalvalue (734 g/L) obtained from the experimental verification at the optimum values. |
format |
Article in Journal/Newspaper |
author |
MEHMETOĞLU, ÜLKÜ KAPUCU, HAMDİ ÇALIMLI, AYLA GÜVENÇ, AFİFE Kapucu, NURCAN |
spellingShingle |
MEHMETOĞLU, ÜLKÜ KAPUCU, HAMDİ ÇALIMLI, AYLA GÜVENÇ, AFİFE Kapucu, NURCAN Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology |
author_facet |
MEHMETOĞLU, ÜLKÜ KAPUCU, HAMDİ ÇALIMLI, AYLA GÜVENÇ, AFİFE Kapucu, NURCAN |
author_sort |
MEHMETOĞLU, ÜLKÜ |
title |
Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology |
title_short |
Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology |
title_full |
Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology |
title_fullStr |
Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology |
title_full_unstemmed |
Lipase catalyzed synthesis of oleyl oleate: Optimization by response surface methodology |
title_sort |
lipase catalyzed synthesis of oleyl oleate: optimization by response surface methodology |
publishDate |
2003 |
url |
https://doi.org/10.1080/00986440390207396 https://avesis.kocaeli.edu.tr/publication/details/58a7f52e-de0d-4f57-937c-b314ff1acbd6/oai |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
58a7f52e-de0d-4f57-937c-b314ff1acbd6 doi:10.1080/00986440390207396 https://avesis.kocaeli.edu.tr/publication/details/58a7f52e-de0d-4f57-937c-b314ff1acbd6/oai |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1080/00986440390207396 |
_version_ |
1766271709887856640 |