On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell
The soluble protein of hemocytes from diseased shell (HDS) of oyster, Crassostrea gigas, was shown to play a key role in the rapid growth of calcium carbonate crystals. In this study, we compared HDS extracted from regenerated (or diseased) shell with bovine carbonic anhydrase II in terms of their a...
Published in: | Micron |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
PERGAMON-ELSEVIER SCIENCE LTD
2008
|
Subjects: | |
Online Access: | http://hdl.handle.net/10203/9655 https://doi.org/10.1016/j.micron.2008.04.005 |
id |
ftkoasas:oai:koasas.kaist.ac.kr:10203/9655 |
---|---|
record_format |
openpolar |
spelling |
ftkoasas:oai:koasas.kaist.ac.kr:10203/9655 2023-05-15T15:58:35+02:00 On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell Lee, SW Lee, Seung Woo Park, SB Park, SeungBin Choi, CS Choi, Cheong Song 200812 http://hdl.handle.net/10203/9655 https://doi.org/10.1016/j.micron.2008.04.005 ENG en_US eng PERGAMON-ELSEVIER SCIENCE LTD ARTICLE A 2008 ftkoasas https://doi.org/10.1016/j.micron.2008.04.005 2013-12-15T18:15:40Z The soluble protein of hemocytes from diseased shell (HDS) of oyster, Crassostrea gigas, was shown to play a key role in the rapid growth of calcium carbonate crystals. In this study, we compared HDS extracted from regenerated (or diseased) shell with bovine carbonic anhydrase II in terms of their ability to promote the growth of calcium carbonate crystals. On the basis of scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis, a high growth rate of calcium carbonate crystals was identified under artificial seawater and atmospheric temperature. The function and role of HDS extracted from regenerated shell are discussed at the molecular point as compared to aragonite-specific soluble proteins. Our findings suggest that hemocytes function as a soluble protein, with repeated GX (G: Gly, X: Asp, Asn or Glu) or negative charged amino acid domains binding calcium and specific surface features for catalyzing rapid shell regeneration. (C) 2008 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Korea Advanced Institute of Science and Technology: KOASAS - KAIST Open Access Self-Archiving System Micron 39 8 1228 1234 |
institution |
Open Polar |
collection |
Korea Advanced Institute of Science and Technology: KOASAS - KAIST Open Access Self-Archiving System |
op_collection_id |
ftkoasas |
language |
English |
description |
The soluble protein of hemocytes from diseased shell (HDS) of oyster, Crassostrea gigas, was shown to play a key role in the rapid growth of calcium carbonate crystals. In this study, we compared HDS extracted from regenerated (or diseased) shell with bovine carbonic anhydrase II in terms of their ability to promote the growth of calcium carbonate crystals. On the basis of scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis, a high growth rate of calcium carbonate crystals was identified under artificial seawater and atmospheric temperature. The function and role of HDS extracted from regenerated shell are discussed at the molecular point as compared to aragonite-specific soluble proteins. Our findings suggest that hemocytes function as a soluble protein, with repeated GX (G: Gly, X: Asp, Asn or Glu) or negative charged amino acid domains binding calcium and specific surface features for catalyzing rapid shell regeneration. (C) 2008 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Lee, SW Lee, Seung Woo Park, SB Park, SeungBin Choi, CS Choi, Cheong Song |
spellingShingle |
Lee, SW Lee, Seung Woo Park, SB Park, SeungBin Choi, CS Choi, Cheong Song On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell |
author_facet |
Lee, SW Lee, Seung Woo Park, SB Park, SeungBin Choi, CS Choi, Cheong Song |
author_sort |
Lee, SW Lee, Seung Woo |
title |
On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell |
title_short |
On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell |
title_full |
On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell |
title_fullStr |
On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell |
title_full_unstemmed |
On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell |
title_sort |
on self-organized shell formation by bovine carbonic anhydrase ii, and soluble protein extracted from regenerated shell |
publisher |
PERGAMON-ELSEVIER SCIENCE LTD |
publishDate |
2008 |
url |
http://hdl.handle.net/10203/9655 https://doi.org/10.1016/j.micron.2008.04.005 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_doi |
https://doi.org/10.1016/j.micron.2008.04.005 |
container_title |
Micron |
container_volume |
39 |
container_issue |
8 |
container_start_page |
1228 |
op_container_end_page |
1234 |
_version_ |
1766394350089011200 |