UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp
The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effe...
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Online Access: | https://pure.knaw.nl/portal/en/publications/ec06ff16-9652-40c6-8539-93f6bf9142aa https://hdl.handle.net/20.500.11755/ec06ff16-9652-40c6-8539-93f6bf9142aa http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/359/ |
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ftknawnlpublic:oai:pure.knaw.nl:publications/ec06ff16-9652-40c6-8539-93f6bf9142aa 2024-09-09T19:10:23+00:00 UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp Buma, A.G.J. Van Hannen, E.J. Veldhuis, M. Gieskes, W.W.C. 1996 https://pure.knaw.nl/portal/en/publications/ec06ff16-9652-40c6-8539-93f6bf9142aa https://hdl.handle.net/20.500.11755/ec06ff16-9652-40c6-8539-93f6bf9142aa http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/359/ eng eng https://pure.knaw.nl/portal/en/publications/ec06ff16-9652-40c6-8539-93f6bf9142aa info:eu-repo/semantics/openAccess Buma , A G J , Van Hannen , E J , Veldhuis , M & Gieskes , W W C 1996 , ' UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp ' , Scientia Marina , vol. 60 , pp. 101-106 . < http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/359/ > article 1996 ftknawnlpublic https://doi.org/20.500.11755/ec06ff16-9652-40c6-8539-93f6bf9142aa 2024-07-22T23:43:54Z The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed. [KEYWORDS: cell cycle; DNA damage; flow-cytometry; marine diatom; thymine dimers; UV-B Ultraviolet-radiation; antarctica; dosimeter; dimers; ocean;cells] The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are ... Article in Journal/Newspaper Antarc* Antarctica Royal Netherlands Academy of Arts and Sciences Research Portal (KNAW) |
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Open Polar |
collection |
Royal Netherlands Academy of Arts and Sciences Research Portal (KNAW) |
op_collection_id |
ftknawnlpublic |
language |
English |
description |
The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed. [KEYWORDS: cell cycle; DNA damage; flow-cytometry; marine diatom; thymine dimers; UV-B Ultraviolet-radiation; antarctica; dosimeter; dimers; ocean;cells] The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are ... |
format |
Article in Journal/Newspaper |
author |
Buma, A.G.J. Van Hannen, E.J. Veldhuis, M. Gieskes, W.W.C. |
spellingShingle |
Buma, A.G.J. Van Hannen, E.J. Veldhuis, M. Gieskes, W.W.C. UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp |
author_facet |
Buma, A.G.J. Van Hannen, E.J. Veldhuis, M. Gieskes, W.W.C. |
author_sort |
Buma, A.G.J. |
title |
UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp |
title_short |
UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp |
title_full |
UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp |
title_fullStr |
UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp |
title_full_unstemmed |
UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp |
title_sort |
uv-b induces dna damage and dna synthesis delay in the marine diatom cyclotella sp |
publishDate |
1996 |
url |
https://pure.knaw.nl/portal/en/publications/ec06ff16-9652-40c6-8539-93f6bf9142aa https://hdl.handle.net/20.500.11755/ec06ff16-9652-40c6-8539-93f6bf9142aa http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/359/ |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Buma , A G J , Van Hannen , E J , Veldhuis , M & Gieskes , W W C 1996 , ' UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp ' , Scientia Marina , vol. 60 , pp. 101-106 . < http://www.icm.csic.es/scimar/index.php/secId/6/IdArt/359/ > |
op_relation |
https://pure.knaw.nl/portal/en/publications/ec06ff16-9652-40c6-8539-93f6bf9142aa |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11755/ec06ff16-9652-40c6-8539-93f6bf9142aa |
_version_ |
1809825381594693632 |