Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches
1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma an...
Published in: | Functional Ecology |
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Online Access: | https://pure.knaw.nl/portal/en/publications/9f56e576-391f-4d54-8b73-fb5011947901 https://doi.org/10.1111/j.1365-2435.2010.01689.x https://hdl.handle.net/20.500.11755/9f56e576-391f-4d54-8b73-fb5011947901 |
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ftknawnlpublic:oai:pure.knaw.nl:publications/9f56e576-391f-4d54-8b73-fb5011947901 2024-04-28T08:15:09+00:00 Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches Klaassen, M.R.J. Piersma, T. Korthals, H.J. Dekinga, A. Dietz, M. 2010 https://pure.knaw.nl/portal/en/publications/9f56e576-391f-4d54-8b73-fb5011947901 https://doi.org/10.1111/j.1365-2435.2010.01689.x https://hdl.handle.net/20.500.11755/9f56e576-391f-4d54-8b73-fb5011947901 eng eng https://pure.knaw.nl/portal/en/publications/9f56e576-391f-4d54-8b73-fb5011947901 info:eu-repo/semantics/closedAccess Klaassen , M R J , Piersma , T , Korthals , H J , Dekinga , A & Dietz , M 2010 , ' Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches ' , Functional Ecology , vol. 24 , no. 4 , pp. 796-804 . https://doi.org/10.1111/j.1365-2435.2010.01689.x article 2010 ftknawnlpublic https://doi.org/10.1111/j.1365-2435.2010.01689.x20.500.11755/9f56e576-391f-4d54-8b73-fb5011947901 2024-04-11T00:11:30Z 1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this ‘isotopic-clock’ principle, we present here a mathematical model that yields an analytical and easily calculated outcome. 2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required. 3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably. 4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations. 5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the ‘time-since-diet-shift’ estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here. 1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the ... Article in Journal/Newspaper Calidris canutus Red Knot KNAW: Research Explorer (Royal Netherlands Academy of Arts and Sciences) Functional Ecology 24 4 796 804 |
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Open Polar |
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KNAW: Research Explorer (Royal Netherlands Academy of Arts and Sciences) |
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English |
description |
1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this ‘isotopic-clock’ principle, we present here a mathematical model that yields an analytical and easily calculated outcome. 2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required. 3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably. 4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations. 5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the ‘time-since-diet-shift’ estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here. 1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the ... |
format |
Article in Journal/Newspaper |
author |
Klaassen, M.R.J. Piersma, T. Korthals, H.J. Dekinga, A. Dietz, M. |
spellingShingle |
Klaassen, M.R.J. Piersma, T. Korthals, H.J. Dekinga, A. Dietz, M. Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
author_facet |
Klaassen, M.R.J. Piersma, T. Korthals, H.J. Dekinga, A. Dietz, M. |
author_sort |
Klaassen, M.R.J. |
title |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_short |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_full |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_fullStr |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_full_unstemmed |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_sort |
single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
publishDate |
2010 |
url |
https://pure.knaw.nl/portal/en/publications/9f56e576-391f-4d54-8b73-fb5011947901 https://doi.org/10.1111/j.1365-2435.2010.01689.x https://hdl.handle.net/20.500.11755/9f56e576-391f-4d54-8b73-fb5011947901 |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_source |
Klaassen , M R J , Piersma , T , Korthals , H J , Dekinga , A & Dietz , M 2010 , ' Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches ' , Functional Ecology , vol. 24 , no. 4 , pp. 796-804 . https://doi.org/10.1111/j.1365-2435.2010.01689.x |
op_relation |
https://pure.knaw.nl/portal/en/publications/9f56e576-391f-4d54-8b73-fb5011947901 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/j.1365-2435.2010.01689.x20.500.11755/9f56e576-391f-4d54-8b73-fb5011947901 |
container_title |
Functional Ecology |
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24 |
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4 |
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804 |
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1797580887209017344 |