Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems
In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypothese...
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2014
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Online Access: | https://pure.knaw.nl/portal/en/publications/00462724-9aa7-4503-988d-e79a0be759a3 https://doi.org/10.1111/nph.12791 https://hdl.handle.net/20.500.11755/00462724-9aa7-4503-988d-e79a0be759a3 |
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ftknawnlpublic:oai:pure.knaw.nl:publications/00462724-9aa7-4503-988d-e79a0be759a3 2024-09-09T19:27:07+00:00 Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems Bodeker, I.T.M. Clemmensen, K.E. De Boer, W. Martin, F. Olson, A. Lindahl, B.D. 2014 https://pure.knaw.nl/portal/en/publications/00462724-9aa7-4503-988d-e79a0be759a3 https://doi.org/10.1111/nph.12791 https://hdl.handle.net/20.500.11755/00462724-9aa7-4503-988d-e79a0be759a3 eng eng https://pure.knaw.nl/portal/en/publications/00462724-9aa7-4503-988d-e79a0be759a3 info:eu-repo/semantics/openAccess Bodeker , I T M , Clemmensen , K E , De Boer , W , Martin , F , Olson , A & Lindahl , B D 2014 , ' Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems ' , New Phytologist , vol. 203 , no. 1 , pp. 245-256 . https://doi.org/10.1111/nph.12791 international article 2014 ftknawnlpublic https://doi.org/10.1111/nph.1279120.500.11755/00462724-9aa7-4503-988d-e79a0be759a3 2024-07-22T23:43:54Z In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypotheses that ectomycorrhizal Cortinarius species produce Mn-dependent peroxidases, and that the activity of these enzymes declines at elevated concentrations of inorganic N. In a boreal pine forest and a sub-arctic birch forest, Cortinarius DNA was assessed by 454-sequencing of ITS amplicons and related to Mn-peroxidase activity in humus samples with- and without previous N amendment. Transcription of Cortinarius Mn-peroxidase genes was investigated in field samples. Phylogenetic analyses of Cortinarius peroxidase amplicons and genome sequences were performed. We found a significant co-localization of high peroxidase activity and DNA from Cortinarius species. Peroxidase activity was reduced by high ammonium concentrations. Amplification of mRNA sequences indicated transcription of Cortinarius Mn-peroxidase genes under field conditions. The Cortinarius glaucopus genome encodes 11 peroxidases - a number comparable to many white-rot wood decomposers. These results support the hypothesis that some ectomycorrhizal fungi - Cortinarius species in particular - may play an important role in decomposition of complex organic matter, linked to their mobilization of organically bound N. [KEYWORDS: carbon sequestration Class II peroxidases decomposition ectomycorrhizal fungi high throughput sequencing nitrogen limitation priming effect transcription SOIL ORGANIC-MATTER DEGRADING HEME PEROXIDASES MYCORRHIZAL FUNGI BOREAL FOREST In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypotheses that ... Article in Journal/Newspaper Arctic Royal Netherlands Academy of Arts and Sciences Research Portal (KNAW) Arctic New Phytologist 203 1 245 256 |
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Royal Netherlands Academy of Arts and Sciences Research Portal (KNAW) |
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language |
English |
topic |
international |
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international Bodeker, I.T.M. Clemmensen, K.E. De Boer, W. Martin, F. Olson, A. Lindahl, B.D. Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems |
topic_facet |
international |
description |
In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypotheses that ectomycorrhizal Cortinarius species produce Mn-dependent peroxidases, and that the activity of these enzymes declines at elevated concentrations of inorganic N. In a boreal pine forest and a sub-arctic birch forest, Cortinarius DNA was assessed by 454-sequencing of ITS amplicons and related to Mn-peroxidase activity in humus samples with- and without previous N amendment. Transcription of Cortinarius Mn-peroxidase genes was investigated in field samples. Phylogenetic analyses of Cortinarius peroxidase amplicons and genome sequences were performed. We found a significant co-localization of high peroxidase activity and DNA from Cortinarius species. Peroxidase activity was reduced by high ammonium concentrations. Amplification of mRNA sequences indicated transcription of Cortinarius Mn-peroxidase genes under field conditions. The Cortinarius glaucopus genome encodes 11 peroxidases - a number comparable to many white-rot wood decomposers. These results support the hypothesis that some ectomycorrhizal fungi - Cortinarius species in particular - may play an important role in decomposition of complex organic matter, linked to their mobilization of organically bound N. [KEYWORDS: carbon sequestration Class II peroxidases decomposition ectomycorrhizal fungi high throughput sequencing nitrogen limitation priming effect transcription SOIL ORGANIC-MATTER DEGRADING HEME PEROXIDASES MYCORRHIZAL FUNGI BOREAL FOREST In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypotheses that ... |
format |
Article in Journal/Newspaper |
author |
Bodeker, I.T.M. Clemmensen, K.E. De Boer, W. Martin, F. Olson, A. Lindahl, B.D. |
author_facet |
Bodeker, I.T.M. Clemmensen, K.E. De Boer, W. Martin, F. Olson, A. Lindahl, B.D. |
author_sort |
Bodeker, I.T.M. |
title |
Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems |
title_short |
Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems |
title_full |
Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems |
title_fullStr |
Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems |
title_full_unstemmed |
Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems |
title_sort |
ectomycorrhizal cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems |
publishDate |
2014 |
url |
https://pure.knaw.nl/portal/en/publications/00462724-9aa7-4503-988d-e79a0be759a3 https://doi.org/10.1111/nph.12791 https://hdl.handle.net/20.500.11755/00462724-9aa7-4503-988d-e79a0be759a3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Bodeker , I T M , Clemmensen , K E , De Boer , W , Martin , F , Olson , A & Lindahl , B D 2014 , ' Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems ' , New Phytologist , vol. 203 , no. 1 , pp. 245-256 . https://doi.org/10.1111/nph.12791 |
op_relation |
https://pure.knaw.nl/portal/en/publications/00462724-9aa7-4503-988d-e79a0be759a3 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/nph.1279120.500.11755/00462724-9aa7-4503-988d-e79a0be759a3 |
container_title |
New Phytologist |
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203 |
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1 |
container_start_page |
245 |
op_container_end_page |
256 |
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1809896616490958848 |