Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds
This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, S...
Published in: | Systematic and Applied Microbiology |
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ftkingscollondon:oai:pure.atira.dk:publications/e7b1754c-7096-4914-b673-b14fd7ff4624 2023-05-15T13:41:41+02:00 Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds Moosvi, S A McDonald, I R Pearce, D A Kelly, D P Wood, A P 2005-08-01 https://kclpure.kcl.ac.uk/portal/en/publications/molecular-detection-and-isolation-from-antarctica-of-methylotrophic-bacteria-able-to-grow-with-methylated-sulfur-compounds(e7b1754c-7096-4914-b673-b14fd7ff4624).html https://doi.org/10.1016/j.syapm.2005.03.002 eng eng info:eu-repo/semantics/restrictedAccess Moosvi , S A , McDonald , I R , Pearce , D A , Kelly , D P & Wood , A P 2005 , ' Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds ' , SYSTEMATIC AND APPLIED MICROBIOLOGY , vol. 28 , no. 6 , pp. 541 - 554 . https://doi.org/10.1016/j.syapm.2005.03.002 article 2005 ftkingscollondon https://doi.org/10.1016/j.syapm.2005.03.002 2022-10-14T09:56:02Z This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, South Orkney Islands, Antarctica. Genes encoding the mxaF of methanol dehydrogenase, the fdxA for Afipia ferredoxin, the msmA of methanesulfonate monooxygenase, and the 16S rRNA gene of Methylobacterium were detected in all samples tested. The mxaF gene sequences corresponded to those of Hyphomicrobium, Methylobacterium, and Methylomonas. Over 30 pure cultures of methylotrophs were isolated on methanesulfonate, dimethylsulfone, or dimethylsulfide from ten Signy Island lakes. Some were identified from 16S rRNA gene sequences (and morphology) as Hyphomicrobium species, strains of Afipia felis, and a methylotrophic Flavobacterium strain. Antarctic environments thus contain diverse methylotrophic bacteria, growing on various C-1-substrates, including C-1-sulfur compounds. (c) 2005 Elsevier GmbH. All rights reserved Article in Journal/Newspaper Antarc* Antarctic Antarctica Signy Island South Orkney Islands King's College, London: Research Portal Antarctic Island Lakes ENVELOPE(-128.226,-128.226,62.344,62.344) Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Systematic and Applied Microbiology 28 6 541 554 |
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Open Polar |
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King's College, London: Research Portal |
op_collection_id |
ftkingscollondon |
language |
English |
description |
This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, South Orkney Islands, Antarctica. Genes encoding the mxaF of methanol dehydrogenase, the fdxA for Afipia ferredoxin, the msmA of methanesulfonate monooxygenase, and the 16S rRNA gene of Methylobacterium were detected in all samples tested. The mxaF gene sequences corresponded to those of Hyphomicrobium, Methylobacterium, and Methylomonas. Over 30 pure cultures of methylotrophs were isolated on methanesulfonate, dimethylsulfone, or dimethylsulfide from ten Signy Island lakes. Some were identified from 16S rRNA gene sequences (and morphology) as Hyphomicrobium species, strains of Afipia felis, and a methylotrophic Flavobacterium strain. Antarctic environments thus contain diverse methylotrophic bacteria, growing on various C-1-substrates, including C-1-sulfur compounds. (c) 2005 Elsevier GmbH. All rights reserved |
format |
Article in Journal/Newspaper |
author |
Moosvi, S A McDonald, I R Pearce, D A Kelly, D P Wood, A P |
spellingShingle |
Moosvi, S A McDonald, I R Pearce, D A Kelly, D P Wood, A P Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
author_facet |
Moosvi, S A McDonald, I R Pearce, D A Kelly, D P Wood, A P |
author_sort |
Moosvi, S A |
title |
Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_short |
Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_full |
Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_fullStr |
Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_full_unstemmed |
Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_sort |
molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
publishDate |
2005 |
url |
https://kclpure.kcl.ac.uk/portal/en/publications/molecular-detection-and-isolation-from-antarctica-of-methylotrophic-bacteria-able-to-grow-with-methylated-sulfur-compounds(e7b1754c-7096-4914-b673-b14fd7ff4624).html https://doi.org/10.1016/j.syapm.2005.03.002 |
long_lat |
ENVELOPE(-128.226,-128.226,62.344,62.344) ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(-45.500,-45.500,-60.583,-60.583) |
geographic |
Antarctic Island Lakes Signy Island South Orkney Islands |
geographic_facet |
Antarctic Island Lakes Signy Island South Orkney Islands |
genre |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
op_source |
Moosvi , S A , McDonald , I R , Pearce , D A , Kelly , D P & Wood , A P 2005 , ' Molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds ' , SYSTEMATIC AND APPLIED MICROBIOLOGY , vol. 28 , no. 6 , pp. 541 - 554 . https://doi.org/10.1016/j.syapm.2005.03.002 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.syapm.2005.03.002 |
container_title |
Systematic and Applied Microbiology |
container_volume |
28 |
container_issue |
6 |
container_start_page |
541 |
op_container_end_page |
554 |
_version_ |
1766153794489417728 |