Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways
Methylmercury (MeHg) is a widely distributed contaminant polluting many aquatic environments, with health risks to humans exposed mainly through consumption of seafood. The mechanisms of toxicity of MeHg are not completely understood. In order to map the range of molecular targets and gain better in...
Published in: | Aquatic Toxicology |
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2013
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Online Access: | https://kclpure.kcl.ac.uk/portal/en/publications/global-transcriptome-analysis-of-atlantic-cod-gadus-morhua-liver-after-in-vivo-methylmercury-exposure-suggests-effects-on-energy-metabolism-pathways(4bcdf87a-e43a-48f5-9e95-d1285ffeaf81).html https://doi.org/10.1016/j.aquatox.2012.09.013 |
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ftkingscollondon:oai:pure.atira.dk:publications/4bcdf87a-e43a-48f5-9e95-d1285ffeaf81 2023-05-15T15:27:10+02:00 Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways Yadetie, Fekadu Karlsen, Odd Andre Lanzen, Anders Berg, Karin Olsvik, Pal Hogstrand, Christer Goksoyr, Anders 2013-01-15 https://kclpure.kcl.ac.uk/portal/en/publications/global-transcriptome-analysis-of-atlantic-cod-gadus-morhua-liver-after-in-vivo-methylmercury-exposure-suggests-effects-on-energy-metabolism-pathways(4bcdf87a-e43a-48f5-9e95-d1285ffeaf81).html https://doi.org/10.1016/j.aquatox.2012.09.013 eng eng info:eu-repo/semantics/restrictedAccess Yadetie , F , Karlsen , O A , Lanzen , A , Berg , K , Olsvik , P , Hogstrand , C & Goksoyr , A 2013 , ' Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways ' , AQUATIC TOXICOLOGY , vol. 126 , pp. 314-325 . https://doi.org/10.1016/j.aquatox.2012.09.013 Microarray Transcription Methylmercury Toxicogenomics Atlantic cod Biomarker ZEBRAFISH DANIO-RERIO GENE-EXPRESSION DIETARY METHYLMERCURY OXIDATIVE STRESS MOUSE-LIVER MERCURY FISH SYSTEM GLUTATHIONE TOXICOLOGY article 2013 ftkingscollondon https://doi.org/10.1016/j.aquatox.2012.09.013 2022-10-14T10:17:18Z Methylmercury (MeHg) is a widely distributed contaminant polluting many aquatic environments, with health risks to humans exposed mainly through consumption of seafood. The mechanisms of toxicity of MeHg are not completely understood. In order to map the range of molecular targets and gain better insights into the mechanisms of toxicity, we prepared Atlantic cod (Gadus morhua) 135k oligonucleotide arrays and performed global analysis of transcriptional changes in the liver of fish treated with MeHg (0.5 and 2 mg/ kg of body weight) for 14 days. Inferring from the observed transcriptional changes, the main pathways significantly affected by the treatment were energy metabolism, oxidative stress response, immune response and cytoskeleton remodeling. Consistent with known effects of MeHg, many transcripts for genes in oxidative stress pathways such as glutathione metabolism and Nrf2 regulation of oxidative stress response were differentially regulated. Among the differentially regulated genes, there were disproportionate numbers of genes coding for enzymes involved in metabolism of amino acids, fatty acids and glucose. In particular, many genes coding for enzymes of fatty acid beta-oxidation were up-regulated. The coordinated effects observed on many transcripts coding for enzymes of energy pathways may suggest disruption of nutrient metabolism by MeHg. Many transcripts for genes coding for enzymes in the synthetic pathways of sulphur containing amino acids were also up-regulated, suggesting adaptive responses to MeHg toxicity. By this toxicogenomics approach, we were also able to identify many potential biomarker candidate genes for monitoring environmental MeHg pollution. These results based on changes on transcript levels, however, need to be confirmed by other methods such as proteomics. (C) 2012 Elsevier B.V. All rights reserved. Article in Journal/Newspaper atlantic cod Gadus morhua King's College, London: Research Portal Aquatic Toxicology 126 314 325 |
institution |
Open Polar |
collection |
King's College, London: Research Portal |
op_collection_id |
ftkingscollondon |
language |
English |
topic |
Microarray Transcription Methylmercury Toxicogenomics Atlantic cod Biomarker ZEBRAFISH DANIO-RERIO GENE-EXPRESSION DIETARY METHYLMERCURY OXIDATIVE STRESS MOUSE-LIVER MERCURY FISH SYSTEM GLUTATHIONE TOXICOLOGY |
spellingShingle |
Microarray Transcription Methylmercury Toxicogenomics Atlantic cod Biomarker ZEBRAFISH DANIO-RERIO GENE-EXPRESSION DIETARY METHYLMERCURY OXIDATIVE STRESS MOUSE-LIVER MERCURY FISH SYSTEM GLUTATHIONE TOXICOLOGY Yadetie, Fekadu Karlsen, Odd Andre Lanzen, Anders Berg, Karin Olsvik, Pal Hogstrand, Christer Goksoyr, Anders Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways |
topic_facet |
Microarray Transcription Methylmercury Toxicogenomics Atlantic cod Biomarker ZEBRAFISH DANIO-RERIO GENE-EXPRESSION DIETARY METHYLMERCURY OXIDATIVE STRESS MOUSE-LIVER MERCURY FISH SYSTEM GLUTATHIONE TOXICOLOGY |
description |
Methylmercury (MeHg) is a widely distributed contaminant polluting many aquatic environments, with health risks to humans exposed mainly through consumption of seafood. The mechanisms of toxicity of MeHg are not completely understood. In order to map the range of molecular targets and gain better insights into the mechanisms of toxicity, we prepared Atlantic cod (Gadus morhua) 135k oligonucleotide arrays and performed global analysis of transcriptional changes in the liver of fish treated with MeHg (0.5 and 2 mg/ kg of body weight) for 14 days. Inferring from the observed transcriptional changes, the main pathways significantly affected by the treatment were energy metabolism, oxidative stress response, immune response and cytoskeleton remodeling. Consistent with known effects of MeHg, many transcripts for genes in oxidative stress pathways such as glutathione metabolism and Nrf2 regulation of oxidative stress response were differentially regulated. Among the differentially regulated genes, there were disproportionate numbers of genes coding for enzymes involved in metabolism of amino acids, fatty acids and glucose. In particular, many genes coding for enzymes of fatty acid beta-oxidation were up-regulated. The coordinated effects observed on many transcripts coding for enzymes of energy pathways may suggest disruption of nutrient metabolism by MeHg. Many transcripts for genes coding for enzymes in the synthetic pathways of sulphur containing amino acids were also up-regulated, suggesting adaptive responses to MeHg toxicity. By this toxicogenomics approach, we were also able to identify many potential biomarker candidate genes for monitoring environmental MeHg pollution. These results based on changes on transcript levels, however, need to be confirmed by other methods such as proteomics. (C) 2012 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Yadetie, Fekadu Karlsen, Odd Andre Lanzen, Anders Berg, Karin Olsvik, Pal Hogstrand, Christer Goksoyr, Anders |
author_facet |
Yadetie, Fekadu Karlsen, Odd Andre Lanzen, Anders Berg, Karin Olsvik, Pal Hogstrand, Christer Goksoyr, Anders |
author_sort |
Yadetie, Fekadu |
title |
Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways |
title_short |
Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways |
title_full |
Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways |
title_fullStr |
Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways |
title_full_unstemmed |
Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways |
title_sort |
global transcriptome analysis of atlantic cod (gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways |
publishDate |
2013 |
url |
https://kclpure.kcl.ac.uk/portal/en/publications/global-transcriptome-analysis-of-atlantic-cod-gadus-morhua-liver-after-in-vivo-methylmercury-exposure-suggests-effects-on-energy-metabolism-pathways(4bcdf87a-e43a-48f5-9e95-d1285ffeaf81).html https://doi.org/10.1016/j.aquatox.2012.09.013 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Yadetie , F , Karlsen , O A , Lanzen , A , Berg , K , Olsvik , P , Hogstrand , C & Goksoyr , A 2013 , ' Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways ' , AQUATIC TOXICOLOGY , vol. 126 , pp. 314-325 . https://doi.org/10.1016/j.aquatox.2012.09.013 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.aquatox.2012.09.013 |
container_title |
Aquatic Toxicology |
container_volume |
126 |
container_start_page |
314 |
op_container_end_page |
325 |
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1766357619153305600 |