Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism
Background: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the most abundant among PCBs detected in animal tissues, its mechanism of toxicity is not well understood. Only few studies have been conducted...
Published in: | BMC Genomics |
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Online Access: | https://kclpure.kcl.ac.uk/portal/en/publications/liver-transcriptome-analysis-of-atlantic-cod-gadus-morhua-exposed-to-pcb-153-indicates-effects-on-cell-cycle-regulation-and-lipid-metabolism(43a05221-5def-4ddc-ba9b-eba4272b5025).html https://doi.org/10.1186/1471-2164-15-481 |
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ftkingscollondon:oai:pure.atira.dk:publications/43a05221-5def-4ddc-ba9b-eba4272b5025 2023-05-15T15:27:20+02:00 Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism Yadetie, Fekadu Karlsen, Odd Andre Eide, Marta Hogstrand, Christer Goksoyr, Anders 2014-06-17 https://kclpure.kcl.ac.uk/portal/en/publications/liver-transcriptome-analysis-of-atlantic-cod-gadus-morhua-exposed-to-pcb-153-indicates-effects-on-cell-cycle-regulation-and-lipid-metabolism(43a05221-5def-4ddc-ba9b-eba4272b5025).html https://doi.org/10.1186/1471-2164-15-481 eng eng info:eu-repo/semantics/openAccess Yadetie , F , Karlsen , O A , Eide , M , Hogstrand , C & Goksoyr , A 2014 , ' Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism ' , BMC GENOMICS , vol. 15 , 481 . https://doi.org/10.1186/1471-2164-15-481 CONSTITUTIVE ANDROSTANE RECEPTOR POLYCHLORINATED-BIPHENYLS PCBS PERSISTENT ORGANIC POLLUTANTS PREGNANE-X-RECEPTOR RESPONSE 1 EGR1 GENE-EXPRESSION ADIPOCYTE DIFFERENTIATION ENVIRONMENTAL CHEMICALS ENDOCRINE DISRUPTORS ENERGY-METABOLISM article 2014 ftkingscollondon https://doi.org/10.1186/1471-2164-15-481 2022-10-14T10:22:15Z Background: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the most abundant among PCBs detected in animal tissues, its mechanism of toxicity is not well understood. Only few studies have been conducted to explore genes and pathways affected by PCB 153 by using high throughput transcriptomics approaches. To obtain better insights into toxicity mechanisms, we treated juvenile Atlantic cod (Gadus morhua) with PCB 153 (0.5, 2 and 8 mg/kg body weight) for 2 weeks and performed gene expression analysis in the liver using oligonucleotide arrays. Results: Whole-genome gene expression analysis detected about 160 differentially regulated genes. Functional enrichment, interactome, network and gene set enrichment analysis of the differentially regulated genes suggested that pathways associated with cell cycle, lipid metabolism, immune response, apoptosis and stress response were among the top significantly enriched. Particularly, genes coding for proteins in DNA replication/cell cycle pathways and enzymes of lipid biosynthesis were up-regulated suggesting increased cell proliferation and lipogenesis, respectively. Conclusions: PCB 153 appears to activate cell proliferation and lipogenic genes in cod liver. Transcriptional up-regulation of marker genes for lipid biosynthesis resembles lipogenic effects previously reported for persistent organic pollutants (POPs) and other environmental chemicals. Our results provide new insights into mechanisms of PCB 153 induced toxicity. Article in Journal/Newspaper atlantic cod Gadus morhua King's College, London: Research Portal BMC Genomics 15 1 481 |
institution |
Open Polar |
collection |
King's College, London: Research Portal |
op_collection_id |
ftkingscollondon |
language |
English |
topic |
CONSTITUTIVE ANDROSTANE RECEPTOR POLYCHLORINATED-BIPHENYLS PCBS PERSISTENT ORGANIC POLLUTANTS PREGNANE-X-RECEPTOR RESPONSE 1 EGR1 GENE-EXPRESSION ADIPOCYTE DIFFERENTIATION ENVIRONMENTAL CHEMICALS ENDOCRINE DISRUPTORS ENERGY-METABOLISM |
spellingShingle |
CONSTITUTIVE ANDROSTANE RECEPTOR POLYCHLORINATED-BIPHENYLS PCBS PERSISTENT ORGANIC POLLUTANTS PREGNANE-X-RECEPTOR RESPONSE 1 EGR1 GENE-EXPRESSION ADIPOCYTE DIFFERENTIATION ENVIRONMENTAL CHEMICALS ENDOCRINE DISRUPTORS ENERGY-METABOLISM Yadetie, Fekadu Karlsen, Odd Andre Eide, Marta Hogstrand, Christer Goksoyr, Anders Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
topic_facet |
CONSTITUTIVE ANDROSTANE RECEPTOR POLYCHLORINATED-BIPHENYLS PCBS PERSISTENT ORGANIC POLLUTANTS PREGNANE-X-RECEPTOR RESPONSE 1 EGR1 GENE-EXPRESSION ADIPOCYTE DIFFERENTIATION ENVIRONMENTAL CHEMICALS ENDOCRINE DISRUPTORS ENERGY-METABOLISM |
description |
Background: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the most abundant among PCBs detected in animal tissues, its mechanism of toxicity is not well understood. Only few studies have been conducted to explore genes and pathways affected by PCB 153 by using high throughput transcriptomics approaches. To obtain better insights into toxicity mechanisms, we treated juvenile Atlantic cod (Gadus morhua) with PCB 153 (0.5, 2 and 8 mg/kg body weight) for 2 weeks and performed gene expression analysis in the liver using oligonucleotide arrays. Results: Whole-genome gene expression analysis detected about 160 differentially regulated genes. Functional enrichment, interactome, network and gene set enrichment analysis of the differentially regulated genes suggested that pathways associated with cell cycle, lipid metabolism, immune response, apoptosis and stress response were among the top significantly enriched. Particularly, genes coding for proteins in DNA replication/cell cycle pathways and enzymes of lipid biosynthesis were up-regulated suggesting increased cell proliferation and lipogenesis, respectively. Conclusions: PCB 153 appears to activate cell proliferation and lipogenic genes in cod liver. Transcriptional up-regulation of marker genes for lipid biosynthesis resembles lipogenic effects previously reported for persistent organic pollutants (POPs) and other environmental chemicals. Our results provide new insights into mechanisms of PCB 153 induced toxicity. |
format |
Article in Journal/Newspaper |
author |
Yadetie, Fekadu Karlsen, Odd Andre Eide, Marta Hogstrand, Christer Goksoyr, Anders |
author_facet |
Yadetie, Fekadu Karlsen, Odd Andre Eide, Marta Hogstrand, Christer Goksoyr, Anders |
author_sort |
Yadetie, Fekadu |
title |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_short |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_full |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_fullStr |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_full_unstemmed |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_sort |
liver transcriptome analysis of atlantic cod (gadus morhua) exposed to pcb 153 indicates effects on cell cycle regulation and lipid metabolism |
publishDate |
2014 |
url |
https://kclpure.kcl.ac.uk/portal/en/publications/liver-transcriptome-analysis-of-atlantic-cod-gadus-morhua-exposed-to-pcb-153-indicates-effects-on-cell-cycle-regulation-and-lipid-metabolism(43a05221-5def-4ddc-ba9b-eba4272b5025).html https://doi.org/10.1186/1471-2164-15-481 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Yadetie , F , Karlsen , O A , Eide , M , Hogstrand , C & Goksoyr , A 2014 , ' Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism ' , BMC GENOMICS , vol. 15 , 481 . https://doi.org/10.1186/1471-2164-15-481 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/1471-2164-15-481 |
container_title |
BMC Genomics |
container_volume |
15 |
container_issue |
1 |
container_start_page |
481 |
_version_ |
1766357775144714240 |