A multiplex assay with 52 single nucleotide polymorphisms for human identification
A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We establi...
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ftkingscollondon:oai:kclpure.kcl.ac.uk:publications/f93fb391-33f4-4f6b-86f9-3324e964faaa 2024-09-15T18:09:51+00:00 A multiplex assay with 52 single nucleotide polymorphisms for human identification Sanchez, Juan J Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels 2006-05 https://kclpure.kcl.ac.uk/portal/en/publications/f93fb391-33f4-4f6b-86f9-3324e964faaa https://doi.org/10.1002/elps.200500671 eng eng https://kclpure.kcl.ac.uk/portal/en/publications/f93fb391-33f4-4f6b-86f9-3324e964faaa info:eu-repo/semantics/restrictedAccess Sanchez , J J , Phillips , C , Børsting , C , Balogh , K , Bogus , M , Fondevila , M , Harrison , C D , Musgrave-Brown , E , Salas , A , Syndercombe Court , D , Schneider , P M , Carracedo , A & Morling , N 2006 , ' A multiplex assay with 52 single nucleotide polymorphisms for human identification ' , Electrophoresis , vol. 27 , no. 9 , pp. 1713-1724 . https://doi.org/10.1002/elps.200500671 article 2006 ftkingscollondon https://doi.org/10.1002/elps.200500671 2024-07-29T23:47:31Z A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously only partial STR profiles had been obtained. A total of 700 individuals from Denmark, Greenland, Somalia, Turkey, China, Germany, Taiwan, Thailand and Japan were typed, and the allele frequencies estimated. All 52 SNPs were polymorphic in the three major population groups. The mean match probability was at least 5.0 x 10(-19) in the populations studied. Typical paternity indices ranged from 336 000 in Asians to 549 000 in Europeans. Details of the 52 SNP loci and population data generated in this work are freely available at http://www.snpforid.org. Article in Journal/Newspaper Greenland King's College, London: Research Portal ELECTROPHORESIS 27 9 1713 1724 |
institution |
Open Polar |
collection |
King's College, London: Research Portal |
op_collection_id |
ftkingscollondon |
language |
English |
description |
A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously only partial STR profiles had been obtained. A total of 700 individuals from Denmark, Greenland, Somalia, Turkey, China, Germany, Taiwan, Thailand and Japan were typed, and the allele frequencies estimated. All 52 SNPs were polymorphic in the three major population groups. The mean match probability was at least 5.0 x 10(-19) in the populations studied. Typical paternity indices ranged from 336 000 in Asians to 549 000 in Europeans. Details of the 52 SNP loci and population data generated in this work are freely available at http://www.snpforid.org. |
format |
Article in Journal/Newspaper |
author |
Sanchez, Juan J Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels |
spellingShingle |
Sanchez, Juan J Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels A multiplex assay with 52 single nucleotide polymorphisms for human identification |
author_facet |
Sanchez, Juan J Phillips, Chris Børsting, Claus Balogh, Kinga Bogus, Magdalena Fondevila, Manuel Harrison, Cheryl D Musgrave-Brown, Esther Salas, Antonio Syndercombe Court, Denise Schneider, Peter M Carracedo, Angel Morling, Niels |
author_sort |
Sanchez, Juan J |
title |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_short |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_full |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_fullStr |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_full_unstemmed |
A multiplex assay with 52 single nucleotide polymorphisms for human identification |
title_sort |
multiplex assay with 52 single nucleotide polymorphisms for human identification |
publishDate |
2006 |
url |
https://kclpure.kcl.ac.uk/portal/en/publications/f93fb391-33f4-4f6b-86f9-3324e964faaa https://doi.org/10.1002/elps.200500671 |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Sanchez , J J , Phillips , C , Børsting , C , Balogh , K , Bogus , M , Fondevila , M , Harrison , C D , Musgrave-Brown , E , Salas , A , Syndercombe Court , D , Schneider , P M , Carracedo , A & Morling , N 2006 , ' A multiplex assay with 52 single nucleotide polymorphisms for human identification ' , Electrophoresis , vol. 27 , no. 9 , pp. 1713-1724 . https://doi.org/10.1002/elps.200500671 |
op_relation |
https://kclpure.kcl.ac.uk/portal/en/publications/f93fb391-33f4-4f6b-86f9-3324e964faaa |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1002/elps.200500671 |
container_title |
ELECTROPHORESIS |
container_volume |
27 |
container_issue |
9 |
container_start_page |
1713 |
op_container_end_page |
1724 |
_version_ |
1810447450401406976 |