Shifts in the coral microbiome in response to in situ experimental deoxygenation

• Main Authors: Howard, Rachel D., Schul, Monica, Rodriguez Bravo, Lucia M., Altieri, Andrew, Meyer, Julie L.
• Other Authors: Red Sea Research Center (RSRC), Department of Soil, Water, and Ecosystem Sciences, University of Florida, Gainesville, FL, USA, Smithsonian Tropical Research Institute, Balboa, Ancon, Republic of Panama, Department of Environmental Engineering Sciences, University of Florida, Gainesville, FL, USA
• Format: Report
• Language: unknown
• Published: Cold Spring Harbor Laboratory 2023
• Subjects: Ocean acidification
• Online Access: http://hdl.handle.net/10754/691087; https://doi.org/10.1101/2023.04.06.535971

Global climate change impacts ocean communities through rising surface temperatures, ocean acidification, and deoxygenation. While the response of the coral holobiont to the first two effects has been relatively well studied, little is known about the response of the coral microbiome to deoxygenation. In this study, we investigated the response of the microbiome to hypoxia in two coral species that differ in their relative tolerance to hypoxia. We conducted in situ oxygen manipulations on a coral reef in Bahía Almirante, Panama, which has previously experienced episodes of low dissolved oxygen concentrations. Naïve coral colonies (previously unexposed to hypoxia) of massive starlet coral (Siderastrea siderea) and Lamarck’s sheet coral (Agaricia lamarcki) were transplanted to a reef and either enclosed in chambers that created hypoxic conditions or left at ambient oxygen levels. We collected samples of surface mucus and tissue after 48 hours of exposure and characterized the microbiome by sequencing 16S rRNA genes. We found that the microbiomes of the two coral species were distinct from one another and remained so after exhibiting similar shifts in microbiome composition in response to hypoxia. There was an increase in both abundance and number of taxa of anaerobic microbes after exposure to hypoxia. Some of these taxa may play beneficial roles in the coral holobiont by detoxifying the surrounding environment during hypoxic stress. This work describes the first characterization of the coral microbiome under hypoxia and is an initial step toward identifying potential beneficial bacteria for corals facing this environmental stressor. We thank the team at the Smithsonian Tropical Research Institute in Bocas del Toro, Panama for their assistance in field monitoring and work. This research was supported by University of Florida start-up funds to AHA and JLM, and NSF grant OCE-2048914 to AHA and JLM.