Mycobacterial diversity in soil samples from King George Island, Antarctica
Mycobacteria are ubiquitous microorganisms and may be found in many different environments, either associated or not to diseases. Culture-independent molecular techniques brought new perspectives for research regarding soil microorganisms, which is relevant to microbiologists and industry. Therefore...
Published in: | Polar Science |
---|---|
Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Elsevier BV
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/10754/682233 https://doi.org/10.1016/j.polar.2022.100890 |
id |
ftkingabdullahun:oai:repository.kaust.edu.sa:10754/682233 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
King Abdullah University of Science and Technology: KAUST Repository |
op_collection_id |
ftkingabdullahun |
language |
unknown |
description |
Mycobacteria are ubiquitous microorganisms and may be found in many different environments, either associated or not to diseases. Culture-independent molecular techniques brought new perspectives for research regarding soil microorganisms, which is relevant to microbiologists and industry. Therefore, new research is necessary to broaden our knowledge about ecology and physiology of mycobacteria in extreme environments. The aim of this study was to verify the presence of mycobacteria in Antarctica soil samples and to standardize molecular and culture-dependent techniques to their isolation. Soil DNA was extracted and submitted to denaturing gradient gel electrophoresis (DGGE), rrs mycobacterial gene amplification and partial sequencing. Thereby, 10 colonies morphologically similar to mycobacteria were isolated from 10 different samples, resulting in 15 DNA sequences where Mycobacterium neglectum and Mycobacterium kumamotonense were identified, suggesting that Mycobacterium terrae complex representatives are ubiquitous in the region. This study proposes a simple method for mycobacteria cultivation from soil samples and shows that ornithogenic soils can be the source of organic matter and low pH, modulating the mycobacterial Antarctica diversity. Further research aiming to explore mycobacterial diversity in soil are needed to increase knowledge and better explore their evolution, their relation with animal and human species and their potential biotechnological use. We would like to thank the Platform-DNA Sequencing (PDTIS/FIOCRUZ), Rio de Janeiro, RJ, Brazil, for performing the rpoB, gyrA and gyrB sequencing procedure; Dr. Leão, for genomic analysis; the AlconLaboratórios do Brasil Ltda, for moxifloxacin donation; and Marlei Gomes da Silva, for his continued help and support throughout lab experiments. This study was supported in part by the Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Projects 110.347/2007, 18123/2008, 111.497/2008, and 110.272/2010), by the Conselho Nacional de ... |
author2 |
Biological and Environmental Science and Engineering (BESE) Division Marine Science Program Red Sea Research Center (RSRC) Bioscience Program Programa de Pos-Graduacao em Ciencias (Microbiologia), Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, RJ, Brazil Laboratorio de Micobacterias, Departamento de Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, RJ, Brazil Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, RJ, Brazil Universidade Federal do Rio de Janeiro, campus Macae, RJ, Brazil Departamento de Microbiologia e Parasitologia, Universidade Federal do Estado do Rio de Janeiro, RJ, Brazil |
format |
Article in Journal/Newspaper |
author |
de Barros, Caroline Reis Baethgen, Ludmila Fiorenzano Mastrobuono, Gabriela Peixoto, Raquel S Miranda, Karla Rodrigues da Silva Carvalho, Ana Carolina da Silva Dias, Rubens Clayton Rosado, Alexandre S. Duarte, Rafael Silva |
spellingShingle |
de Barros, Caroline Reis Baethgen, Ludmila Fiorenzano Mastrobuono, Gabriela Peixoto, Raquel S Miranda, Karla Rodrigues da Silva Carvalho, Ana Carolina da Silva Dias, Rubens Clayton Rosado, Alexandre S. Duarte, Rafael Silva Mycobacterial diversity in soil samples from King George Island, Antarctica |
author_facet |
de Barros, Caroline Reis Baethgen, Ludmila Fiorenzano Mastrobuono, Gabriela Peixoto, Raquel S Miranda, Karla Rodrigues da Silva Carvalho, Ana Carolina da Silva Dias, Rubens Clayton Rosado, Alexandre S. Duarte, Rafael Silva |
author_sort |
de Barros, Caroline Reis |
title |
Mycobacterial diversity in soil samples from King George Island, Antarctica |
title_short |
Mycobacterial diversity in soil samples from King George Island, Antarctica |
title_full |
Mycobacterial diversity in soil samples from King George Island, Antarctica |
title_fullStr |
Mycobacterial diversity in soil samples from King George Island, Antarctica |
title_full_unstemmed |
Mycobacterial diversity in soil samples from King George Island, Antarctica |
title_sort |
mycobacterial diversity in soil samples from king george island, antarctica |
publisher |
Elsevier BV |
publishDate |
2022 |
url |
http://hdl.handle.net/10754/682233 https://doi.org/10.1016/j.polar.2022.100890 |
geographic |
King George Island |
geographic_facet |
King George Island |
genre |
Antarc* Antarctica King George Island Polar Science Polar Science |
genre_facet |
Antarc* Antarctica King George Island Polar Science Polar Science |
op_relation |
https://linkinghub.elsevier.com/retrieve/pii/S1873965222001621 de Barros, C. R., Baethgen, L. F., Mastrobuono, G., Peixoto, R. S., Miranda, K. R., da Silva Carvalho, A. C., da Silva Dias, R. C., Rosado, A. S., & Duarte, R. S. (2022). Mycobacterial diversity in soil samples from King George Island, Antarctica. Polar Science, 100890. https://doi.org/10.1016/j.polar.2022.100890 doi:10.1016/j.polar.2022.100890 2-s2.0-85138588150 1873-9652 Polar Science 100890 http://hdl.handle.net/10754/682233 |
op_rights |
NOTICE: this is the author’s version of a work that was accepted for publication in Polar Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Polar Science, [, , (2022-09-05)] DOI:10.1016/j.polar.2022.100890 . © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ 2024-09-05 |
op_doi |
https://doi.org/10.1016/j.polar.2022.100890 |
container_title |
Polar Science |
container_volume |
34 |
container_start_page |
100890 |
_version_ |
1787430527751421952 |
spelling |
ftkingabdullahun:oai:repository.kaust.edu.sa:10754/682233 2024-01-07T09:40:07+01:00 Mycobacterial diversity in soil samples from King George Island, Antarctica de Barros, Caroline Reis Baethgen, Ludmila Fiorenzano Mastrobuono, Gabriela Peixoto, Raquel S Miranda, Karla Rodrigues da Silva Carvalho, Ana Carolina da Silva Dias, Rubens Clayton Rosado, Alexandre S. Duarte, Rafael Silva Biological and Environmental Science and Engineering (BESE) Division Marine Science Program Red Sea Research Center (RSRC) Bioscience Program Programa de Pos-Graduacao em Ciencias (Microbiologia), Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, RJ, Brazil Laboratorio de Micobacterias, Departamento de Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, RJ, Brazil Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, RJ, Brazil Universidade Federal do Rio de Janeiro, campus Macae, RJ, Brazil Departamento de Microbiologia e Parasitologia, Universidade Federal do Estado do Rio de Janeiro, RJ, Brazil 2022-09-05 http://hdl.handle.net/10754/682233 https://doi.org/10.1016/j.polar.2022.100890 unknown Elsevier BV https://linkinghub.elsevier.com/retrieve/pii/S1873965222001621 de Barros, C. R., Baethgen, L. F., Mastrobuono, G., Peixoto, R. S., Miranda, K. R., da Silva Carvalho, A. C., da Silva Dias, R. C., Rosado, A. S., & Duarte, R. S. (2022). Mycobacterial diversity in soil samples from King George Island, Antarctica. Polar Science, 100890. https://doi.org/10.1016/j.polar.2022.100890 doi:10.1016/j.polar.2022.100890 2-s2.0-85138588150 1873-9652 Polar Science 100890 http://hdl.handle.net/10754/682233 NOTICE: this is the author’s version of a work that was accepted for publication in Polar Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Polar Science, [, , (2022-09-05)] DOI:10.1016/j.polar.2022.100890 . © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ 2024-09-05 Article 2022 ftkingabdullahun https://doi.org/10.1016/j.polar.2022.100890 2023-12-09T20:18:33Z Mycobacteria are ubiquitous microorganisms and may be found in many different environments, either associated or not to diseases. Culture-independent molecular techniques brought new perspectives for research regarding soil microorganisms, which is relevant to microbiologists and industry. Therefore, new research is necessary to broaden our knowledge about ecology and physiology of mycobacteria in extreme environments. The aim of this study was to verify the presence of mycobacteria in Antarctica soil samples and to standardize molecular and culture-dependent techniques to their isolation. Soil DNA was extracted and submitted to denaturing gradient gel electrophoresis (DGGE), rrs mycobacterial gene amplification and partial sequencing. Thereby, 10 colonies morphologically similar to mycobacteria were isolated from 10 different samples, resulting in 15 DNA sequences where Mycobacterium neglectum and Mycobacterium kumamotonense were identified, suggesting that Mycobacterium terrae complex representatives are ubiquitous in the region. This study proposes a simple method for mycobacteria cultivation from soil samples and shows that ornithogenic soils can be the source of organic matter and low pH, modulating the mycobacterial Antarctica diversity. Further research aiming to explore mycobacterial diversity in soil are needed to increase knowledge and better explore their evolution, their relation with animal and human species and their potential biotechnological use. We would like to thank the Platform-DNA Sequencing (PDTIS/FIOCRUZ), Rio de Janeiro, RJ, Brazil, for performing the rpoB, gyrA and gyrB sequencing procedure; Dr. Leão, for genomic analysis; the AlconLaboratórios do Brasil Ltda, for moxifloxacin donation; and Marlei Gomes da Silva, for his continued help and support throughout lab experiments. This study was supported in part by the Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Projects 110.347/2007, 18123/2008, 111.497/2008, and 110.272/2010), by the Conselho Nacional de ... Article in Journal/Newspaper Antarc* Antarctica King George Island Polar Science Polar Science King Abdullah University of Science and Technology: KAUST Repository King George Island Polar Science 34 100890 |