Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization
Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs)...
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Online Access: | http://hdl.handle.net/10754/681966 https://doi.org/10.3390/antiox11101906 |
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ftkingabdullahun:oai:repository.kaust.edu.sa:10754/681966 2024-01-07T09:39:58+01:00 Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. Biological and Environmental Science and Engineering Division, Water Desalination and Reuse Research Center, King Abdullah University of Science and Technology, Riyadh 11543, Saudi Arabia Water Desalination and Reuse Research Center (WDRC) Biological and Environmental Science and Engineering (BESE) Division 2022-09-26 application/pdf http://hdl.handle.net/10754/681966 https://doi.org/10.3390/antiox11101906 unknown MDPI AG https://www.mdpi.com/2076-3921/11/10/1906 Ming, S., Li, S., Chen, Z., Chen, X., Wang, F., Deng, S., Marszałek, K., Zhu, Z., Zhang, W., & Barba, F. J. (2022). Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization. Antioxidants, 11(10), 1906. https://doi.org/10.3390/antiox11101906 doi:10.3390/antiox11101906 2076-3921 10 Antioxidants 1906 http://hdl.handle.net/10754/681966 11 Archived with thanks to Antioxidants under a Creative Commons license, details at: https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Article 2022 ftkingabdullahun https://doi.org/10.3390/antiox11101906 2023-12-09T20:18:48Z Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs), including CAL-B@PES, CAL-B@PDA/PES and GA/CAL-B@PDA/PES— were fabricated to improve the antioxidant flavanone glycoside hesperidin lipophilization. Via reverse filtration, PDA coating and GA crosslinking, Candida antarctica lipase B (CAL-B) was stably immobilized on membrane to fabricate BLIMs. Among the three BLIMs, GA/CAL-B@PDA/PES had the greatest enzyme activity and enzyme loading, the strongest tolerance of changes in external environmental conditions (temperatures, pH, heating time, storage time and numbers of cycles) and the highest hesperidin esterification efficiency. Moreover, the optimal operating condition for GA/CAL-B@PDA/PES fabrication was the CAL-B concentration of 0.36 mg/mL, operation pressure of 2 bar, GA concentration of 5% and crosslinking time of 1 h. Afterwards, the hesperidin esterification process did not affect the micromorphology of BLIM, but clearly improved the BLIM permeability and esterified product efficiency. The present study reveals the fabrication mechanism of BLIMs and offers insights into the optimizing strategy that governs the membrane-based lipophilization technology process. F.J.B. is member of the CYTED network “P320RT0186—Aprovechamiento sostenible de recursos biomásicos vegetales iberoamericanos en cosmética (BIOLATES)”. This research was funded by the Outstanding Young and Middle-aged Science and Technology Innovation Team in Hubei Province (T2020012), Major Technology Innovation of Hubei Province (2019ABA113), National Natural Science Foundation of China (51908136 and 22178136), Key Research and Development Program of Hubei Province (2020BBA043) and “One Hundred-Talent Program” of Hubei Province, China. Article in Journal/Newspaper Antarc* Antarctica King Abdullah University of Science and Technology: KAUST Repository Antioxidants 11 10 1906 |
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King Abdullah University of Science and Technology: KAUST Repository |
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ftkingabdullahun |
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description |
Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs), including CAL-B@PES, CAL-B@PDA/PES and GA/CAL-B@PDA/PES— were fabricated to improve the antioxidant flavanone glycoside hesperidin lipophilization. Via reverse filtration, PDA coating and GA crosslinking, Candida antarctica lipase B (CAL-B) was stably immobilized on membrane to fabricate BLIMs. Among the three BLIMs, GA/CAL-B@PDA/PES had the greatest enzyme activity and enzyme loading, the strongest tolerance of changes in external environmental conditions (temperatures, pH, heating time, storage time and numbers of cycles) and the highest hesperidin esterification efficiency. Moreover, the optimal operating condition for GA/CAL-B@PDA/PES fabrication was the CAL-B concentration of 0.36 mg/mL, operation pressure of 2 bar, GA concentration of 5% and crosslinking time of 1 h. Afterwards, the hesperidin esterification process did not affect the micromorphology of BLIM, but clearly improved the BLIM permeability and esterified product efficiency. The present study reveals the fabrication mechanism of BLIMs and offers insights into the optimizing strategy that governs the membrane-based lipophilization technology process. F.J.B. is member of the CYTED network “P320RT0186—Aprovechamiento sostenible de recursos biomásicos vegetales iberoamericanos en cosmética (BIOLATES)”. This research was funded by the Outstanding Young and Middle-aged Science and Technology Innovation Team in Hubei Province (T2020012), Major Technology Innovation of Hubei Province (2019ABA113), National Natural Science Foundation of China (51908136 and 22178136), Key Research and Development Program of Hubei Province (2020BBA043) and “One Hundred-Talent Program” of Hubei Province, China. |
author2 |
Biological and Environmental Science and Engineering Division, Water Desalination and Reuse Research Center, King Abdullah University of Science and Technology, Riyadh 11543, Saudi Arabia Water Desalination and Reuse Research Center (WDRC) Biological and Environmental Science and Engineering (BESE) Division |
format |
Article in Journal/Newspaper |
author |
Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. |
spellingShingle |
Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
author_facet |
Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. |
author_sort |
Ming, Shanxiu |
title |
Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_short |
Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_full |
Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_fullStr |
Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_full_unstemmed |
Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_sort |
bioinspired lipase immobilized membrane for improving hesperidin lipophilization |
publisher |
MDPI AG |
publishDate |
2022 |
url |
http://hdl.handle.net/10754/681966 https://doi.org/10.3390/antiox11101906 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://www.mdpi.com/2076-3921/11/10/1906 Ming, S., Li, S., Chen, Z., Chen, X., Wang, F., Deng, S., Marszałek, K., Zhu, Z., Zhang, W., & Barba, F. J. (2022). Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization. Antioxidants, 11(10), 1906. https://doi.org/10.3390/antiox11101906 doi:10.3390/antiox11101906 2076-3921 10 Antioxidants 1906 http://hdl.handle.net/10754/681966 11 |
op_rights |
Archived with thanks to Antioxidants under a Creative Commons license, details at: https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/antiox11101906 |
container_title |
Antioxidants |
container_volume |
11 |
container_issue |
10 |
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1906 |
_version_ |
1787430298858815488 |