Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives

Macroalgae are key primary producers in North Atlantic and Arctic coastal ecosystems, and tracing their fate and distribution is vital to improve our understanding of their ecological role and provision of ecosystem services. Recent advances from environmental DNA (eDNA) have added a new capacity to...

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Published in:Environmental DNA
Main Authors: Ørberg, Sarah B., Krause-Jensen, Dorte, Geraldi, Nathan, Ortega, Alejandra, Diaz Rua, Ruben, Duarte, Carlos M.
Other Authors: Biological and Environmental Science and Engineering (BESE) Division, Bioscience Program, Computational Bioscience Research Center (CBRC), Marine Science Program, NGS and qPCR, Red Sea Research Center (RSRC), Strategic National Advancement, Young Talent Development, Department of Bioscience Aarhus University Silkeborg Denmark, Arctic Research Centre Aarhus University Aarhus C Denmark
Format: Article in Journal/Newspaper
Language:unknown
Published: Wiley 2021
Subjects:
Online Access:http://hdl.handle.net/10754/673325
https://doi.org/10.1002/edn3.262
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spelling ftkingabdullahun:oai:repository.kaust.edu.sa:10754/673325 2024-01-07T09:40:33+01:00 Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives Ørberg, Sarah B. Krause-Jensen, Dorte Geraldi, Nathan Ortega, Alejandra Diaz Rua, Ruben Duarte, Carlos M. Biological and Environmental Science and Engineering (BESE) Division Bioscience Program Computational Bioscience Research Center (CBRC) Marine Science Program NGS and qPCR Red Sea Research Center (RSRC) Strategic National Advancement Young Talent Development Department of Bioscience Aarhus University Silkeborg Denmark Arctic Research Centre Aarhus University Aarhus C Denmark 2021-11-11T11:42:38Z application/pdf http://hdl.handle.net/10754/673325 https://doi.org/10.1002/edn3.262 unknown Wiley https://onlinelibrary.wiley.com/doi/10.1002/edn3.262 Ørberg, S. B., Krause-Jensen, D., Geraldi, N. R., Ortega, A., Díaz-Rúa, R., & Duarte, C. M. (2021). Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives. Environmental DNA. doi:10.1002/edn3.262 doi:10.1002/edn3.262 2637-4943 Environmental DNA http://hdl.handle.net/10754/673325 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. http://creativecommons.org/licenses/by-nc-nd/4.0/ Article 2021 ftkingabdullahun https://doi.org/10.1002/edn3.262 2023-12-09T20:18:52Z Macroalgae are key primary producers in North Atlantic and Arctic coastal ecosystems, and tracing their fate and distribution is vital to improve our understanding of their ecological role and provision of ecosystem services. Recent advances from environmental DNA (eDNA) have added a new capacity to fingerprint and trace macroalgae. However, further development of resources for amplifying and identifying macroalgal eDNA are much needed. Here, we examined the performance in terms of resolution and specificity of two 18S primers (18S-V7 and 18S-V9) recently applied in identifying macroalgae from eDNA. We also built a local barcode database for primer 18S-V7 with 31 widespread Arctic and North Atlantic macroalgal species to complement the existing DNA databases. Furthermore, we applied metabarcoding of eDNA to identify macroalgae in Arctic marine sediments (Disko Bay, W. Greenland) and evaluated the contributions from our local barcode database. We identified macroalgal DNA from 19 families across 11 orders in surface (0–1 cm, with both primers) and sub-surface (5–10 cm, with 18S-V7 primer) sediments. The barcode database developed here with the 18S-V7 primer improved the identification of unique families, from 16 to 19 families, thereby strengthening the taxonomic assignment possible relative to pre-existing barcode reference sequences. Overall, this study demonstrates the feasibility of eDNA to resolve contributions of macroalgae in Arctic marine sediments, and enhances the fingerprinting resolution. We thereby document a novel pathway to answer key questions on the ecological role and fate of macroalgae in the Arctic. This research was supported by the Independent Research Fund Denmark (8021-00222 B, ‘CARMA’) to DK-J and King Abdullah University of Science and Technology through baseline funding provided to CMD. We thank Susse Wegeberg, Ole Geertz-Hansen, Karsten Dahl, Peter Stæhr and Michael Bo Rasmussen for help with collection and identification of algae and Wajitha J. Raja Mohamed Sait and Nadia Haj Salah ... Article in Journal/Newspaper Arctic Arctic Disko Bay Greenland North Atlantic King Abdullah University of Science and Technology: KAUST Repository Arctic Greenland Rasmussen ENVELOPE(-64.084,-64.084,-65.248,-65.248) Environmental DNA 4 2 385 401
institution Open Polar
collection King Abdullah University of Science and Technology: KAUST Repository
op_collection_id ftkingabdullahun
language unknown
description Macroalgae are key primary producers in North Atlantic and Arctic coastal ecosystems, and tracing their fate and distribution is vital to improve our understanding of their ecological role and provision of ecosystem services. Recent advances from environmental DNA (eDNA) have added a new capacity to fingerprint and trace macroalgae. However, further development of resources for amplifying and identifying macroalgal eDNA are much needed. Here, we examined the performance in terms of resolution and specificity of two 18S primers (18S-V7 and 18S-V9) recently applied in identifying macroalgae from eDNA. We also built a local barcode database for primer 18S-V7 with 31 widespread Arctic and North Atlantic macroalgal species to complement the existing DNA databases. Furthermore, we applied metabarcoding of eDNA to identify macroalgae in Arctic marine sediments (Disko Bay, W. Greenland) and evaluated the contributions from our local barcode database. We identified macroalgal DNA from 19 families across 11 orders in surface (0–1 cm, with both primers) and sub-surface (5–10 cm, with 18S-V7 primer) sediments. The barcode database developed here with the 18S-V7 primer improved the identification of unique families, from 16 to 19 families, thereby strengthening the taxonomic assignment possible relative to pre-existing barcode reference sequences. Overall, this study demonstrates the feasibility of eDNA to resolve contributions of macroalgae in Arctic marine sediments, and enhances the fingerprinting resolution. We thereby document a novel pathway to answer key questions on the ecological role and fate of macroalgae in the Arctic. This research was supported by the Independent Research Fund Denmark (8021-00222 B, ‘CARMA’) to DK-J and King Abdullah University of Science and Technology through baseline funding provided to CMD. We thank Susse Wegeberg, Ole Geertz-Hansen, Karsten Dahl, Peter Stæhr and Michael Bo Rasmussen for help with collection and identification of algae and Wajitha J. Raja Mohamed Sait and Nadia Haj Salah ...
author2 Biological and Environmental Science and Engineering (BESE) Division
Bioscience Program
Computational Bioscience Research Center (CBRC)
Marine Science Program
NGS and qPCR
Red Sea Research Center (RSRC)
Strategic National Advancement
Young Talent Development
Department of Bioscience Aarhus University Silkeborg Denmark
Arctic Research Centre Aarhus University Aarhus C Denmark
format Article in Journal/Newspaper
author Ørberg, Sarah B.
Krause-Jensen, Dorte
Geraldi, Nathan
Ortega, Alejandra
Diaz Rua, Ruben
Duarte, Carlos M.
spellingShingle Ørberg, Sarah B.
Krause-Jensen, Dorte
Geraldi, Nathan
Ortega, Alejandra
Diaz Rua, Ruben
Duarte, Carlos M.
Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives
author_facet Ørberg, Sarah B.
Krause-Jensen, Dorte
Geraldi, Nathan
Ortega, Alejandra
Diaz Rua, Ruben
Duarte, Carlos M.
author_sort Ørberg, Sarah B.
title Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives
title_short Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives
title_full Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives
title_fullStr Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives
title_full_unstemmed Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives
title_sort fingerprinting arctic and north atlantic macroalgae with edna – application and perspectives
publisher Wiley
publishDate 2021
url http://hdl.handle.net/10754/673325
https://doi.org/10.1002/edn3.262
long_lat ENVELOPE(-64.084,-64.084,-65.248,-65.248)
geographic Arctic
Greenland
Rasmussen
geographic_facet Arctic
Greenland
Rasmussen
genre Arctic
Arctic
Disko Bay
Greenland
North Atlantic
genre_facet Arctic
Arctic
Disko Bay
Greenland
North Atlantic
op_relation https://onlinelibrary.wiley.com/doi/10.1002/edn3.262
Ørberg, S. B., Krause-Jensen, D., Geraldi, N. R., Ortega, A., Díaz-Rúa, R., & Duarte, C. M. (2021). Fingerprinting Arctic and North Atlantic Macroalgae with eDNA – Application and perspectives. Environmental DNA. doi:10.1002/edn3.262
doi:10.1002/edn3.262
2637-4943
Environmental DNA
http://hdl.handle.net/10754/673325
op_rights This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
http://creativecommons.org/licenses/by-nc-nd/4.0/
op_doi https://doi.org/10.1002/edn3.262
container_title Environmental DNA
container_volume 4
container_issue 2
container_start_page 385
op_container_end_page 401
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