Seawater carbonate chemistry and gene expression of a reef fish Acanthochromis polyacanthus, supplement to: Schunter, Celia; Welch, Megan J; Nilsson, Göran E; Rummer, Jodie L; Munday, Philip L; Ravasi, Timothy (2018): An interplay between plasticity and parental phenotype determines impacts of ocean acidification on a reef fish. Nature Ecology & Evolution, 2(2), 334-342

• Main Authors: Schunter, Celia Marei, Welch, Megan J., Nilsson, Göran E., Rummer, Jodie L., Munday, Philip L., Ravasi, Timothy
• Other Authors: Biological and Environmental Sciences and Engineering (BESE) Division, Bioscience Program, Integrative Systems Biology Lab, ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia., Section for Physiology and Cell Biology, Department of Biosciences, University of Oslo, Oslo, Norway.
• Format: Dataset
• Language: unknown
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2019
• Subjects: Acanthochromis polyacanthus; Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20- 1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Nekton; Pelagos; Single species; South Pacific; Tropical; Type; Species; Registration number of species; Uniform resource locator/link to reference; Figure; Treatment; Gene name; Gene expression; pH; standard deviation; Temperature; water; Salinity; Alkalinity; total; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Bicarbonate ion; Carbonate ion; Carbon; inorganic; dissolved; Aragonite saturation state; Calcite saturation state; Experiment; Pacific; Ocean acidification
• Online Access: http://hdl.handle.net/10754/663946; https://doi.org/10.1594/pangaea.900202

The impacts of ocean acidification will depend on the ability of marine organisms to tolerate, acclimate and eventually adapt to changes in ocean chemistry. Here, we use a unique transgenerational experiment to determine the molecular response of a coral reef fish to short-term, developmental and transgenerational exposure to elevated CO2, and to test how these responses are influenced by variations in tolerance to elevated CO2 exhibited by the parents. Within-generation responses in gene expression to end-of-century predicted CO2 levels indicate that a self-amplifying cycle in GABAergic neurotransmission is triggered, explaining previously reported neurological and behavioural impairments. Furthermore, epigenetic regulator genes exhibited a within-generation specific response, but with some divergence due to parental phenotype. Importantly, we find that altered gene expression for the majority of within-generation responses returns to baseline levels following parental exposure to elevated CO2 conditions. Our results show that both parental variation in tolerance and cross-generation exposure to elevated CO2 are crucial factors in determining the response of reef fish to changing ocean chemistry.