Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B
The immobilization of Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase (TeSADH) using sol–gel method enables its use to racemize enantiopure alcohols in organic media. Here, we report the racemization of enantiopure phenyl-ring-containing secondary alcohols using xerogel-immobilized W1...
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Online Access: | http://hdl.handle.net/10754/622392 https://doi.org/10.1039/c6ra18895h |
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ftkingabdullahun:oai:repository.kaust.edu.sa:10754/622392 2023-12-31T10:01:09+01:00 Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B Karume, Ibrahim Musa, Musa M. Bsharat, Odey Takahashi, Masateru Hamdan, Samir El Ali, Bassam Biological and Environmental Sciences and Engineering (BESE) Division Bioscience Program Department of Chemistry, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia 2016 http://hdl.handle.net/10754/622392 https://doi.org/10.1039/c6ra18895h unknown Royal Society of Chemistry (RSC) http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA18895H Karume I, Musa MM, Bsharat O, Takahashi M, Hamdan SM, et al. (2016) Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B. RSC Adv 6: 96616–96622. Available: http://dx.doi.org/10.1039/c6ra18895h. doi:10.1039/c6ra18895h 2046-2069 RSC Advances http://hdl.handle.net/10754/622392 Article 2016 ftkingabdullahun https://doi.org/10.1039/c6ra18895h 2023-12-02T20:18:26Z The immobilization of Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase (TeSADH) using sol–gel method enables its use to racemize enantiopure alcohols in organic media. Here, we report the racemization of enantiopure phenyl-ring-containing secondary alcohols using xerogel-immobilized W110A TeSADH in hexane rather than the aqueous medium required by the enzyme. We further showed that this racemization approach in organic solvent was compatible with Candida antarctica lipase B (CALB)-catalyzed kinetic resolution. This compatibility, therefore, allowed a dual enzymatic dynamic kinetic resolution of racemic alcohols using CALB-catalyzed kinetic resolution and W110A TeSADH-catalyzed racemization of phenyl-ring-containing alcohols. The authors acknowledge the support provided by King Abdulaziz City for Science and Technology (KACST) through the Science and Technology Unit at King Fahd University of Petroleum and Minerals (KFUPM), for funding this work through project No. 11-BIO1666-04, as part of the National Science, Technology, and Innovation Plan as well as baseline research funding offered to Prof. Hamdan through King Abdullah University of Science and Technology. The authors thank Prof. Claire Vieille, from the Department of Microbiology and Molecular Genetics at Michigan State University, for providing the plasmids of TeSADH. Article in Journal/Newspaper Antarc* Antarctica King Abdullah University of Science and Technology: KAUST Repository RSC Advances 6 99 96616 96622 |
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King Abdullah University of Science and Technology: KAUST Repository |
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ftkingabdullahun |
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description |
The immobilization of Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase (TeSADH) using sol–gel method enables its use to racemize enantiopure alcohols in organic media. Here, we report the racemization of enantiopure phenyl-ring-containing secondary alcohols using xerogel-immobilized W110A TeSADH in hexane rather than the aqueous medium required by the enzyme. We further showed that this racemization approach in organic solvent was compatible with Candida antarctica lipase B (CALB)-catalyzed kinetic resolution. This compatibility, therefore, allowed a dual enzymatic dynamic kinetic resolution of racemic alcohols using CALB-catalyzed kinetic resolution and W110A TeSADH-catalyzed racemization of phenyl-ring-containing alcohols. The authors acknowledge the support provided by King Abdulaziz City for Science and Technology (KACST) through the Science and Technology Unit at King Fahd University of Petroleum and Minerals (KFUPM), for funding this work through project No. 11-BIO1666-04, as part of the National Science, Technology, and Innovation Plan as well as baseline research funding offered to Prof. Hamdan through King Abdullah University of Science and Technology. The authors thank Prof. Claire Vieille, from the Department of Microbiology and Molecular Genetics at Michigan State University, for providing the plasmids of TeSADH. |
author2 |
Biological and Environmental Sciences and Engineering (BESE) Division Bioscience Program Department of Chemistry, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia |
format |
Article in Journal/Newspaper |
author |
Karume, Ibrahim Musa, Musa M. Bsharat, Odey Takahashi, Masateru Hamdan, Samir El Ali, Bassam |
spellingShingle |
Karume, Ibrahim Musa, Musa M. Bsharat, Odey Takahashi, Masateru Hamdan, Samir El Ali, Bassam Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B |
author_facet |
Karume, Ibrahim Musa, Musa M. Bsharat, Odey Takahashi, Masateru Hamdan, Samir El Ali, Bassam |
author_sort |
Karume, Ibrahim |
title |
Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B |
title_short |
Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B |
title_full |
Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B |
title_fullStr |
Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B |
title_full_unstemmed |
Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B |
title_sort |
dual enzymatic dynamic kinetic resolution by thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and candida antarctica lipase b |
publisher |
Royal Society of Chemistry (RSC) |
publishDate |
2016 |
url |
http://hdl.handle.net/10754/622392 https://doi.org/10.1039/c6ra18895h |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C6RA18895H Karume I, Musa MM, Bsharat O, Takahashi M, Hamdan SM, et al. (2016) Dual enzymatic dynamic kinetic resolution by Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase and Candida antarctica lipase B. RSC Adv 6: 96616–96622. Available: http://dx.doi.org/10.1039/c6ra18895h. doi:10.1039/c6ra18895h 2046-2069 RSC Advances http://hdl.handle.net/10754/622392 |
op_doi |
https://doi.org/10.1039/c6ra18895h |
container_title |
RSC Advances |
container_volume |
6 |
container_issue |
99 |
container_start_page |
96616 |
op_container_end_page |
96622 |
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1786795937506525184 |