A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss)
In many vertebrate species visible melanin-based pigmentation patterns correlate with high stress- and disease-resistance, but proximate mechanisms for this trait association remain enigmatic. Here we show that a missense mutation in a classical pigmentation gene, melanocyte stimulating hormone rece...
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Springer Nature
2016
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Online Access: | http://hdl.handle.net/10754/615930 https://doi.org/10.1038/srep28969 |
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King Abdullah University of Science and Technology: KAUST Repository |
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ftkingabdullahun |
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English |
description |
In many vertebrate species visible melanin-based pigmentation patterns correlate with high stress- and disease-resistance, but proximate mechanisms for this trait association remain enigmatic. Here we show that a missense mutation in a classical pigmentation gene, melanocyte stimulating hormone receptor (MC1R), is strongly associated with distinct differences in steroidogenic melanocortin 2 receptor (MC2R) mRNA expression between high- (HR) and low-responsive (LR) rainbow trout (Oncorhynchus mykiss). We also show experimentally that cortisol implants increase the expression of agouti signaling protein (ASIP) mRNA in skin, likely explaining the association between HR-traits and reduced skin melanin patterning. Molecular dynamics simulations predict that melanocortin 2 receptor accessory protein (MRAP), needed for MC2R function, binds differently to the two MC1R variants. Considering that mRNA for MC2R and the MC1R variants are present in head kidney cells, we hypothesized that MC2R activity is modulated in part by different binding affinities of the MC1R variants for MRAP. Experiments in mammalian cells confirmed that trout MRAP interacts with the two trout MC1R variants and MC2R, but failed to detect regulation of MC2R signaling, possibly due to high constitutive MC1R activity. We are deeply thankful to Tom Pottinger for making the HR-LR rainbow trout strains available for this study. We thank Jon K. Lærdahl for making a structural model of MC1R, Christina Sørensen for helping with the cortisol analysis and Ida G. Lunde for help with the figures. Primer and competitor design for the real competitive PCR analysis was kindly performed by Sequenom Custom Service, Hamburg, Germany. The project was financially supported by the Norwegian University of Life Sciences, by Research Council of Norway projects no. 172609, 199728, 218534 and by National Institutes of Health Grant DK19974 (P.M.H.). Atlantic Salmon genomic sequences used for primer design was kindly provided by the International Cooperation to Sequence the ... |
author2 |
King Abdullah University of Science and Technology (KAUST) KAUST Catalysis Center (KCC) Centre for Integrative Genetics, Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, NO-1430 Ås, Norway University of Rochester Medical Center, School of Medicine and Dentistry, Department of Pharmacology and Physiology, Rochester, NY 14642, USA Department of Molecular Biosciences, University of Oslo, NO-0316 Oslo, Norway Norwegian School of Veterinary Medicine, Institute of Basal Sciences and Aquatic Medicine, NO- 0033 Oslo, Norway Technical University of Denmark, National Institute of Aquatic Resources, DK-9850 Hirtshals, Denmark Norwegian Institute for Water Research (NIVA), NO-0349 Oslo, Norway NTNU - Norwegian University of Science and Technology, NO-7491 Trondheim, Norway |
format |
Article in Journal/Newspaper |
author |
Khan, Uniza Wahid Øverli, Øyvind Hinkle, Patricia M. Pasha, Farhan Ahmad Johansen, Ida Beitnes Berget, Ingunn Silva, Patricia I. M. Kittilsen, Silje Höglund, Erik Omholt, Stig W. Våge, Dag Inge |
spellingShingle |
Khan, Uniza Wahid Øverli, Øyvind Hinkle, Patricia M. Pasha, Farhan Ahmad Johansen, Ida Beitnes Berget, Ingunn Silva, Patricia I. M. Kittilsen, Silje Höglund, Erik Omholt, Stig W. Våge, Dag Inge A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) |
author_facet |
Khan, Uniza Wahid Øverli, Øyvind Hinkle, Patricia M. Pasha, Farhan Ahmad Johansen, Ida Beitnes Berget, Ingunn Silva, Patricia I. M. Kittilsen, Silje Höglund, Erik Omholt, Stig W. Våge, Dag Inge |
author_sort |
Khan, Uniza Wahid |
title |
A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) |
title_short |
A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) |
title_full |
A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) |
title_fullStr |
A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) |
title_full_unstemmed |
A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) |
title_sort |
novel role for pigment genes in the stress response in rainbow trout (oncorhynchus mykiss) |
publisher |
Springer Nature |
publishDate |
2016 |
url |
http://hdl.handle.net/10754/615930 https://doi.org/10.1038/srep28969 |
genre |
Atlantic salmon Lunde |
genre_facet |
Atlantic salmon Lunde |
op_relation |
http://www.nature.com/articles/srep28969 A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) 2016, 6:28969 Scientific Reports doi:10.1038/srep28969 2045-2322 Scientific Reports 27373344 http://hdl.handle.net/10754/615930 |
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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1038/srep28969 |
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Scientific Reports |
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1786836269457735680 |
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ftkingabdullahun:oai:repository.kaust.edu.sa:10754/615930 2023-12-31T10:04:50+01:00 A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) Khan, Uniza Wahid Øverli, Øyvind Hinkle, Patricia M. Pasha, Farhan Ahmad Johansen, Ida Beitnes Berget, Ingunn Silva, Patricia I. M. Kittilsen, Silje Höglund, Erik Omholt, Stig W. Våge, Dag Inge King Abdullah University of Science and Technology (KAUST) KAUST Catalysis Center (KCC) Centre for Integrative Genetics, Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, NO-1430 Ås, Norway University of Rochester Medical Center, School of Medicine and Dentistry, Department of Pharmacology and Physiology, Rochester, NY 14642, USA Department of Molecular Biosciences, University of Oslo, NO-0316 Oslo, Norway Norwegian School of Veterinary Medicine, Institute of Basal Sciences and Aquatic Medicine, NO- 0033 Oslo, Norway Technical University of Denmark, National Institute of Aquatic Resources, DK-9850 Hirtshals, Denmark Norwegian Institute for Water Research (NIVA), NO-0349 Oslo, Norway NTNU - Norwegian University of Science and Technology, NO-7491 Trondheim, Norway 2016-07-04 application/pdf http://hdl.handle.net/10754/615930 https://doi.org/10.1038/srep28969 en eng Springer Nature http://www.nature.com/articles/srep28969 A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) 2016, 6:28969 Scientific Reports doi:10.1038/srep28969 2045-2322 Scientific Reports 27373344 http://hdl.handle.net/10754/615930 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Article 2016 ftkingabdullahun https://doi.org/10.1038/srep28969 2023-12-02T20:18:13Z In many vertebrate species visible melanin-based pigmentation patterns correlate with high stress- and disease-resistance, but proximate mechanisms for this trait association remain enigmatic. Here we show that a missense mutation in a classical pigmentation gene, melanocyte stimulating hormone receptor (MC1R), is strongly associated with distinct differences in steroidogenic melanocortin 2 receptor (MC2R) mRNA expression between high- (HR) and low-responsive (LR) rainbow trout (Oncorhynchus mykiss). We also show experimentally that cortisol implants increase the expression of agouti signaling protein (ASIP) mRNA in skin, likely explaining the association between HR-traits and reduced skin melanin patterning. Molecular dynamics simulations predict that melanocortin 2 receptor accessory protein (MRAP), needed for MC2R function, binds differently to the two MC1R variants. Considering that mRNA for MC2R and the MC1R variants are present in head kidney cells, we hypothesized that MC2R activity is modulated in part by different binding affinities of the MC1R variants for MRAP. Experiments in mammalian cells confirmed that trout MRAP interacts with the two trout MC1R variants and MC2R, but failed to detect regulation of MC2R signaling, possibly due to high constitutive MC1R activity. We are deeply thankful to Tom Pottinger for making the HR-LR rainbow trout strains available for this study. We thank Jon K. Lærdahl for making a structural model of MC1R, Christina Sørensen for helping with the cortisol analysis and Ida G. Lunde for help with the figures. Primer and competitor design for the real competitive PCR analysis was kindly performed by Sequenom Custom Service, Hamburg, Germany. The project was financially supported by the Norwegian University of Life Sciences, by Research Council of Norway projects no. 172609, 199728, 218534 and by National Institutes of Health Grant DK19974 (P.M.H.). Atlantic Salmon genomic sequences used for primer design was kindly provided by the International Cooperation to Sequence the ... Article in Journal/Newspaper Atlantic salmon Lunde King Abdullah University of Science and Technology: KAUST Repository Scientific Reports 6 1 |