Set-Up and Validation of a Dynamic Solid/Gas Bioreactor
The limited availability of fossil resourses mandates the development of new energy vectors, which is one of the Grand Challenges of the 21st Century [1]. Biocatalytic energy conversion is a promising solution to meet the increased energy demand of industrialized societies. Applications of biocataly...
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ftkingabdullahun:oai:repository.kaust.edu.sa:10754/224713 2023-12-03T10:13:03+01:00 Set-Up and Validation of a Dynamic Solid/Gas Bioreactor Lloyd-Randol, Jennifer D. Eppinger, Jörg Pinnau, Ingo Takanabe, Kazuhiro Physical Science and Engineering (PSE) Division 2012-05 application/pdf http://hdl.handle.net/10754/224713 https://doi.org/10.25781/KAUST-D8A6Y en eng Lloyd-Randol, J. D. (2012). Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. KAUST Research Repository. https://doi.org/10.25781/KAUST-D8A6Y doi:10.25781/KAUST-D8A6Y http://hdl.handle.net/10754/224713 Biocatalytic Validation Solid gas Bioreactor Immobilization Thesis 2012 ftkingabdullahun https://doi.org/10.25781/KAUST-D8A6Y 2023-11-04T20:26:06Z The limited availability of fossil resourses mandates the development of new energy vectors, which is one of the Grand Challenges of the 21st Century [1]. Biocatalytic energy conversion is a promising solution to meet the increased energy demand of industrialized societies. Applications of biocatalysis in the gas-phase are so far limited to production of fine chemicals and pharmaceuticals. However, this technology has the potential for large scale biocatalytic applications [2], e.g. for the formation of novel energy carriers. The so-called solid/gas biocatalysis is defined as the application of a biocatalyst immobilized on solid-phase support acting on gaseous substrates [3]. This process combines the advantages of bio-catalysis (green chemistry, mild reaction conditions, high specicity & selectivity) and heterogeneous dynamic gas-phase processes (low diffusion limitation, high conversion, simple scale-up). This work presents the modifications of a PID Microactivity Reference reactor in order to make it suitable for solid/gas biocatalysis. The reactor design requirements are based on previously published laboratory scale solid/gas systems with a feed of saturated vapors [4]. These vapors are produced in saturation flasks, which were designed and optimized during this project. Other modifications included relocation of the gas mixing chamber, redesigning the location and heating mechanism for the reactor tube, and heating of the outlet gas line. The modified reactor system was verified based on the Candida antarctica lipase B catalyzed transesterication of ethyl acetate with 1-hexanol to hexyl acetate and ethanol and results were compared to liquid-phase model reactions. Products were analyzed on line by a gas chromatograph with a flame ionization detector. C. antarc- tica physisorbed on silica particles produced a 50% conversion of hexanol at 40 C in the gas-phase. A commercial immobilized lipase from Iris Biotech produced 99% and 97% conversions of hexanol in similar experiments. This project achieved its ... Thesis Antarc* Antarctica King Abdullah University of Science and Technology: KAUST Repository |
institution |
Open Polar |
collection |
King Abdullah University of Science and Technology: KAUST Repository |
op_collection_id |
ftkingabdullahun |
language |
English |
topic |
Biocatalytic Validation Solid gas Bioreactor Immobilization |
spellingShingle |
Biocatalytic Validation Solid gas Bioreactor Immobilization Lloyd-Randol, Jennifer D. Set-Up and Validation of a Dynamic Solid/Gas Bioreactor |
topic_facet |
Biocatalytic Validation Solid gas Bioreactor Immobilization |
description |
The limited availability of fossil resourses mandates the development of new energy vectors, which is one of the Grand Challenges of the 21st Century [1]. Biocatalytic energy conversion is a promising solution to meet the increased energy demand of industrialized societies. Applications of biocatalysis in the gas-phase are so far limited to production of fine chemicals and pharmaceuticals. However, this technology has the potential for large scale biocatalytic applications [2], e.g. for the formation of novel energy carriers. The so-called solid/gas biocatalysis is defined as the application of a biocatalyst immobilized on solid-phase support acting on gaseous substrates [3]. This process combines the advantages of bio-catalysis (green chemistry, mild reaction conditions, high specicity & selectivity) and heterogeneous dynamic gas-phase processes (low diffusion limitation, high conversion, simple scale-up). This work presents the modifications of a PID Microactivity Reference reactor in order to make it suitable for solid/gas biocatalysis. The reactor design requirements are based on previously published laboratory scale solid/gas systems with a feed of saturated vapors [4]. These vapors are produced in saturation flasks, which were designed and optimized during this project. Other modifications included relocation of the gas mixing chamber, redesigning the location and heating mechanism for the reactor tube, and heating of the outlet gas line. The modified reactor system was verified based on the Candida antarctica lipase B catalyzed transesterication of ethyl acetate with 1-hexanol to hexyl acetate and ethanol and results were compared to liquid-phase model reactions. Products were analyzed on line by a gas chromatograph with a flame ionization detector. C. antarc- tica physisorbed on silica particles produced a 50% conversion of hexanol at 40 C in the gas-phase. A commercial immobilized lipase from Iris Biotech produced 99% and 97% conversions of hexanol in similar experiments. This project achieved its ... |
author2 |
Eppinger, Jörg Pinnau, Ingo Takanabe, Kazuhiro Physical Science and Engineering (PSE) Division |
format |
Thesis |
author |
Lloyd-Randol, Jennifer D. |
author_facet |
Lloyd-Randol, Jennifer D. |
author_sort |
Lloyd-Randol, Jennifer D. |
title |
Set-Up and Validation of a Dynamic Solid/Gas Bioreactor |
title_short |
Set-Up and Validation of a Dynamic Solid/Gas Bioreactor |
title_full |
Set-Up and Validation of a Dynamic Solid/Gas Bioreactor |
title_fullStr |
Set-Up and Validation of a Dynamic Solid/Gas Bioreactor |
title_full_unstemmed |
Set-Up and Validation of a Dynamic Solid/Gas Bioreactor |
title_sort |
set-up and validation of a dynamic solid/gas bioreactor |
publishDate |
2012 |
url |
http://hdl.handle.net/10754/224713 https://doi.org/10.25781/KAUST-D8A6Y |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Lloyd-Randol, J. D. (2012). Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. KAUST Research Repository. https://doi.org/10.25781/KAUST-D8A6Y doi:10.25781/KAUST-D8A6Y http://hdl.handle.net/10754/224713 |
op_doi |
https://doi.org/10.25781/KAUST-D8A6Y |
_version_ |
1784259712564330496 |