Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species
Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cy...
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ftkentuniv:oai:kar.kent.ac.uk:99074 2023-12-17T10:28:29+01:00 Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species de Souza, Marcelo Santos Barcellos, Suziane Alves Dos Santos, Michelly da Silva Gunski, Ricardo José Garnero, Analía Del Valle de Oliveira, Edivaldo Herculano Corrêa O'Connor, Rebecca E Griffin, Darren K. Kretschmer, Rafael 2022-11-06 application/pdf https://kar.kent.ac.uk/99074/ https://kar.kent.ac.uk/99074/1/article.pdf https://doi.org/10.3390/ani12213052 en eng MDPI https://kar.kent.ac.uk/99074/1/article.pdf de Souza, Marcelo Santos, Barcellos, Suziane Alves, Dos Santos, Michelly da Silva, Gunski, Ricardo José, Garnero, Analía Del Valle, de Oliveira, Edivaldo Herculano Corrêa, O'Connor, Rebecca E, Griffin, Darren K., Kretschmer, Rafael (2022) Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species. Animals, 12 (21). Article Number 3052. ISSN 2076-2615. (doi:10.3390/ani12213052 <https://doi.org/10.3390/ani12213052>) (KAR id:99074 </99074>) cc_by QH301 Biology Article PeerReviewed 2022 ftkentuniv https://doi.org/10.3390/ani12213052 2023-11-23T23:45:33Z Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds—Calidris canutus, Jacana jacana, and Vanellus chilensis—combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C. canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V. chilensis. We also described a new chromosome number for C. canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species. Article in Journal/Newspaper Calidris canutus University of Kent: KAR - Kent Academic Repository Animals 12 21 3052 |
institution |
Open Polar |
collection |
University of Kent: KAR - Kent Academic Repository |
op_collection_id |
ftkentuniv |
language |
English |
topic |
QH301 Biology |
spellingShingle |
QH301 Biology de Souza, Marcelo Santos Barcellos, Suziane Alves Dos Santos, Michelly da Silva Gunski, Ricardo José Garnero, Analía Del Valle de Oliveira, Edivaldo Herculano Corrêa O'Connor, Rebecca E Griffin, Darren K. Kretschmer, Rafael Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
topic_facet |
QH301 Biology |
description |
Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds—Calidris canutus, Jacana jacana, and Vanellus chilensis—combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C. canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V. chilensis. We also described a new chromosome number for C. canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species. |
format |
Article in Journal/Newspaper |
author |
de Souza, Marcelo Santos Barcellos, Suziane Alves Dos Santos, Michelly da Silva Gunski, Ricardo José Garnero, Analía Del Valle de Oliveira, Edivaldo Herculano Corrêa O'Connor, Rebecca E Griffin, Darren K. Kretschmer, Rafael |
author_facet |
de Souza, Marcelo Santos Barcellos, Suziane Alves Dos Santos, Michelly da Silva Gunski, Ricardo José Garnero, Analía Del Valle de Oliveira, Edivaldo Herculano Corrêa O'Connor, Rebecca E Griffin, Darren K. Kretschmer, Rafael |
author_sort |
de Souza, Marcelo Santos |
title |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_short |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_full |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_fullStr |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_full_unstemmed |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_sort |
microchromosome bac-fish reveals different patterns of genome organization in three charadriiformes species |
publisher |
MDPI |
publishDate |
2022 |
url |
https://kar.kent.ac.uk/99074/ https://kar.kent.ac.uk/99074/1/article.pdf https://doi.org/10.3390/ani12213052 |
genre |
Calidris canutus |
genre_facet |
Calidris canutus |
op_relation |
https://kar.kent.ac.uk/99074/1/article.pdf de Souza, Marcelo Santos, Barcellos, Suziane Alves, Dos Santos, Michelly da Silva, Gunski, Ricardo José, Garnero, Analía Del Valle, de Oliveira, Edivaldo Herculano Corrêa, O'Connor, Rebecca E, Griffin, Darren K., Kretschmer, Rafael (2022) Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species. Animals, 12 (21). Article Number 3052. ISSN 2076-2615. (doi:10.3390/ani12213052 <https://doi.org/10.3390/ani12213052>) (KAR id:99074 </99074>) |
op_rights |
cc_by |
op_doi |
https://doi.org/10.3390/ani12213052 |
container_title |
Animals |
container_volume |
12 |
container_issue |
21 |
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3052 |
_version_ |
1785580609069907968 |