NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles
© 2014 Elsevier B.V. All rights reserved. The "Arctic" point mutation of the Alzheimer's amyloid β-peptide is a rare mutation leading to an early onset of Alzheimer's disease. The peptide may interact with neuronal membranes, where it can provide its toxic effects. We used 2D NMR...
Main Authors: | , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2014
|
Subjects: | |
Online Access: | http://dspace.kpfu.ru/xmlui/handle/net/101148 |
id |
ftkazanuniv:oai:dspace.kpfu.ru:net/101148 |
---|---|
record_format |
openpolar |
spelling |
ftkazanuniv:oai:dspace.kpfu.ru:net/101148 2023-05-15T14:34:16+02:00 NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles Usachev K. Filippov A. Khairutdinov B. Antzutkin O. Klochkov V. 2014 http://dspace.kpfu.ru/xmlui/handle/net/101148 unknown Journal of Molecular Structure 518 1076 http://dspace.kpfu.ru/xmlui/bitstream/net/101148/1/SCOPUS00222860-2014-1076-SID84907050433-p1.pdf 0022-2860 http://dspace.kpfu.ru/xmlui/handle/net/101148 SCOPUS00222860-2014-1076-SID84907050433 "Arctic" mutation Amyloid β-peptide NMR SDS micelle Structure Article 2014 ftkazanuniv 2022-01-01T09:35:13Z © 2014 Elsevier B.V. All rights reserved. The "Arctic" point mutation of the Alzheimer's amyloid β-peptide is a rare mutation leading to an early onset of Alzheimer's disease. The peptide may interact with neuronal membranes, where it can provide its toxic effects. We used 2D NMR spectroscopy to investigate the conformation of the "Arctic" mutant of Aβ1-40 Alzheimer's amyloid peptide in sodium dodecyl sulfate micelle solutions, which are the type of amphiphilic structures mimicking some properties of biomembranes. The study showed that the Arctic mutant of Aβ1-40 interacts with the surface of SDS micelles mainly through the Leu17-Asn27 310-helical region, while the Ile31-Val40 region is buried in the hydrophobic interior of the micelle. In contrast, wild-type Aβ1-40 interacts with SDS micelles through the Lys16-Asp23 α-helical region and Gly29-Met35. Both the Arctic mutant and the wild-type Aβ1-40 peptides interactions with SDS micelles are hydrophobic in nature. Aβ peptides are thought to be capable of forming pores in biomembranes that can cause changes in neuronal and endothelial cell membrane permeability. It has also been shown that Aβ peptides containing the "Arctic" mutation are more neurotoxic and aggregate more readily than the wild-type Aβ peptides at physiological conditions. Here, we propose that the extension of the helical structure of Leu17-Asn27 and a high aliphaticity (neutrality) of the C-terminal region in the Arctic Aβ peptides are consistent with the idea that formation of ion-permeable pores by Aβ oligomers may be one of prevailing mechanisms of a larger neuronal toxicity of the Arctic Aβ compared to the wild-type Aβ peptides, independent of oxidative damage and lipid peroxidation. Article in Journal/Newspaper Arctic Kazan Federal University Digital Repository Arctic |
institution |
Open Polar |
collection |
Kazan Federal University Digital Repository |
op_collection_id |
ftkazanuniv |
language |
unknown |
topic |
"Arctic" mutation Amyloid β-peptide NMR SDS micelle Structure |
spellingShingle |
"Arctic" mutation Amyloid β-peptide NMR SDS micelle Structure Usachev K. Filippov A. Khairutdinov B. Antzutkin O. Klochkov V. NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles |
topic_facet |
"Arctic" mutation Amyloid β-peptide NMR SDS micelle Structure |
description |
© 2014 Elsevier B.V. All rights reserved. The "Arctic" point mutation of the Alzheimer's amyloid β-peptide is a rare mutation leading to an early onset of Alzheimer's disease. The peptide may interact with neuronal membranes, where it can provide its toxic effects. We used 2D NMR spectroscopy to investigate the conformation of the "Arctic" mutant of Aβ1-40 Alzheimer's amyloid peptide in sodium dodecyl sulfate micelle solutions, which are the type of amphiphilic structures mimicking some properties of biomembranes. The study showed that the Arctic mutant of Aβ1-40 interacts with the surface of SDS micelles mainly through the Leu17-Asn27 310-helical region, while the Ile31-Val40 region is buried in the hydrophobic interior of the micelle. In contrast, wild-type Aβ1-40 interacts with SDS micelles through the Lys16-Asp23 α-helical region and Gly29-Met35. Both the Arctic mutant and the wild-type Aβ1-40 peptides interactions with SDS micelles are hydrophobic in nature. Aβ peptides are thought to be capable of forming pores in biomembranes that can cause changes in neuronal and endothelial cell membrane permeability. It has also been shown that Aβ peptides containing the "Arctic" mutation are more neurotoxic and aggregate more readily than the wild-type Aβ peptides at physiological conditions. Here, we propose that the extension of the helical structure of Leu17-Asn27 and a high aliphaticity (neutrality) of the C-terminal region in the Arctic Aβ peptides are consistent with the idea that formation of ion-permeable pores by Aβ oligomers may be one of prevailing mechanisms of a larger neuronal toxicity of the Arctic Aβ compared to the wild-type Aβ peptides, independent of oxidative damage and lipid peroxidation. |
format |
Article in Journal/Newspaper |
author |
Usachev K. Filippov A. Khairutdinov B. Antzutkin O. Klochkov V. |
author_facet |
Usachev K. Filippov A. Khairutdinov B. Antzutkin O. Klochkov V. |
author_sort |
Usachev K. |
title |
NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles |
title_short |
NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles |
title_full |
NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles |
title_fullStr |
NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles |
title_full_unstemmed |
NMR structure of the Arctic mutation of the Alzheimer's Aβ(1-40) peptide docked to SDS micelles |
title_sort |
nmr structure of the arctic mutation of the alzheimer's aβ(1-40) peptide docked to sds micelles |
publishDate |
2014 |
url |
http://dspace.kpfu.ru/xmlui/handle/net/101148 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
SCOPUS00222860-2014-1076-SID84907050433 |
op_relation |
Journal of Molecular Structure 518 1076 http://dspace.kpfu.ru/xmlui/bitstream/net/101148/1/SCOPUS00222860-2014-1076-SID84907050433-p1.pdf 0022-2860 http://dspace.kpfu.ru/xmlui/handle/net/101148 |
_version_ |
1766307344598171648 |