Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure
Secondary conformational analysis via Circular Dichroism (CD) and Amide-I FTIR was applied to preparations of Candida antarctica Lipase B (CALB), subtilisin Carlsberg, and the Lipase from Thermomyces lanuginosus (TLL) on fumed silica to confirm that the “hardness” and packing density of the enzymes...
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ftkansassu:oai:krex.k-state.edu:2097/4486 2023-05-15T14:02:43+02:00 Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure Cruz, Juan C. Pfromm, Peter H. Tomich, John M. Rezac, Mary E. pfromm rezac jtomich 2010 application/pdf http://hdl.handle.net/2097/4486 unknown https://doi.org/10.1016/j.colsurfb.2010.06.005 http://hdl.handle.net/2097/4486 This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ Conformational Stability Adsorption Fumed Silica Protein-surface interactions FTIR CD Text 2010 ftkansassu https://doi.org/10.1016/j.colsurfb.2010.06.005 2022-03-05T18:32:14Z Secondary conformational analysis via Circular Dichroism (CD) and Amide-I FTIR was applied to preparations of Candida antarctica Lipase B (CALB), subtilisin Carlsberg, and the Lipase from Thermomyces lanuginosus (TLL) on fumed silica to confirm that the “hardness” and packing density of the enzymes on the solid fumed silica nanoparticle surface can be used to rationalize the variable enzyme-dependent changes of catalytic competency with surface coverage. “Soft” enzymes should be immobilized at a surface coverage where enzyme-enzyme interactions predominate thereby preventing detrimental structural changes caused by enzyme-support interactions, while “hard” enzymes can be immobilized at low to intermediate surface coverage with good catalytic performance. Multi-layered coverage reduces the superficial average catalytic performance in all cases due to mass transfer limitations. Text Antarc* Antarctica Kansas State University: K-State Research Exchange (K-REx) Colloids and Surfaces B: Biointerfaces 81 1 1 10 |
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Open Polar |
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Kansas State University: K-State Research Exchange (K-REx) |
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ftkansassu |
language |
unknown |
topic |
Conformational Stability Adsorption Fumed Silica Protein-surface interactions FTIR CD |
spellingShingle |
Conformational Stability Adsorption Fumed Silica Protein-surface interactions FTIR CD Cruz, Juan C. Pfromm, Peter H. Tomich, John M. Rezac, Mary E. Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure |
topic_facet |
Conformational Stability Adsorption Fumed Silica Protein-surface interactions FTIR CD |
description |
Secondary conformational analysis via Circular Dichroism (CD) and Amide-I FTIR was applied to preparations of Candida antarctica Lipase B (CALB), subtilisin Carlsberg, and the Lipase from Thermomyces lanuginosus (TLL) on fumed silica to confirm that the “hardness” and packing density of the enzymes on the solid fumed silica nanoparticle surface can be used to rationalize the variable enzyme-dependent changes of catalytic competency with surface coverage. “Soft” enzymes should be immobilized at a surface coverage where enzyme-enzyme interactions predominate thereby preventing detrimental structural changes caused by enzyme-support interactions, while “hard” enzymes can be immobilized at low to intermediate surface coverage with good catalytic performance. Multi-layered coverage reduces the superficial average catalytic performance in all cases due to mass transfer limitations. |
author2 |
pfromm rezac jtomich |
format |
Text |
author |
Cruz, Juan C. Pfromm, Peter H. Tomich, John M. Rezac, Mary E. |
author_facet |
Cruz, Juan C. Pfromm, Peter H. Tomich, John M. Rezac, Mary E. |
author_sort |
Cruz, Juan C. |
title |
Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure |
title_short |
Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure |
title_full |
Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure |
title_fullStr |
Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure |
title_full_unstemmed |
Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure |
title_sort |
conformational changes and catalytic competency of hydrolases adsorbing on fumed silica nanoparticles: ii. secondary structure |
publishDate |
2010 |
url |
http://hdl.handle.net/2097/4486 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://doi.org/10.1016/j.colsurfb.2010.06.005 http://hdl.handle.net/2097/4486 |
op_rights |
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/ |
op_doi |
https://doi.org/10.1016/j.colsurfb.2010.06.005 |
container_title |
Colloids and Surfaces B: Biointerfaces |
container_volume |
81 |
container_issue |
1 |
container_start_page |
1 |
op_container_end_page |
10 |
_version_ |
1766273141061976064 |