ナンキョクオキアミのフッ素取り込み機構の分子基盤の解明

• Main Authors: 関 友信, 松田 乾, 馬久地 みゆき, 鈴木 道生, 鈴木 信雄, 大平 剛, セキ トモノブ, マツダ ツヨシ, メクチ ミユキ, スズキ ミチオ, スズキ ノブオ, オオヒラ ツヨシ
• Format: Report
• Language: Japanese
• Published: 神奈川大学総合理学研究所 2020
• Subjects: iron complex; dibenzothiophene derivatives; N,C,S-tridentate ligand; quinolyl group; naphthyl group; charge transfer transition; electronic spectrum; Antarctic; The Antarctic; Antarc*; Antarctic Krill; Euphausia superba
• Online Access: http://hdl.handle.net/10487/00016662; https://kanagawa-u.repo.nii.ac.jp/?action=repository_uri&item_id=13575; https://kanagawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=13575&item_no=1&attribute_id=18&file_no=1

Generally, the exoskeleton of crustaceans is mineralized with calcium carbonate. However, a high concentration of fluorapatite, which is one of the calcium phosphate crystals, is deposited in the exoskeleton of the Antarctic krill Euphausia superba . In order to identify exoskeletal proteins involved in fluoride deposition from E. superba, the tail fan and swimming legs with the epidermis inside the exoskeleton were subjected to RNA-seq analysis. We successfully predicted nine transcripts encoding exoskeletal proteins similar to calcificationassociated peptide-1 (CAP-1), CAP-2, calcification-associated soluble protein-2 (CASP-2), and strong chitin-binding protein-1 (SCBP-1) from the red swamp crayfish Procambarus clarkii, crustocalcin from the kuruma prawn Marsupenaeus japonicus, exoskeletal proteins HACP127 and HACP188 from the American lobster Homarus americanus, arthrodial cuticle protein AMP8.1 from the blue crab Callinectes sapidus, and cuticle protein AM/CP1114 from the crab Cancer pagurus. These transcriptomic data will promote further studies on the functional roles of the exoskeletal proteins involved in fluoride deposition in E. superba. 原著 2019年度神奈川大学総合理学研究所共同研究助成論文