PCR-制限酵素断片長多型法を用いたウナギ加工食品の原料の魚種判定

• Main Authors: 角田, 香澄, 354, ツノダ, カスミ, 9000006954276, 松元, 圭太郎, 401, マツモト, ケイタロウ, 9000003208992
• Language: Japanese
• Published: 2013
• Subjects: うなぎ，魚種判定，PCR-制限酵素断片長多型法; eel; species identification; PCR-RFLP method; Anguilla anguilla; European eel
• Online Access: https://kjunshin.repo.nii.ac.jp/record/260/files/TSUNODA2-KA17.pdf

P(論文) 食品の産地や品種の偽装問題が後を絶たず，食の安全性は社会問題となっている。産地偽装が度々報告され，食資源の枯渇が懸念されているウナギの加工食品について，学生実験（食品衛生学実験・食品学実験）の中でPCR-制限酵素断片長多型（PCR-RFLP）法を用いて原料の魚種判定を行った。薩摩川内市内の食料品店および飲食店で入手したウナギ加工食品（11試料）のうち，４試料が日本などのアジア原産のAnguilla japonica（ジャポニカ種）であり，５試料がヨーロッパ原産のAnguilla anguilla（アンギラ種）であると判定され，残り２試料については判定が出来なかった。中国産と表示・販売されていた３試料はすべてアンギラ種であり，国産と表示・販売されていた３試料のうち２試料はジャポニカ種であり，１試料は判定不能であった。以上の結果，PCR-RFLP法によるウナギの魚種判定は，学生実験レベルの技術でも実施できる簡便な科学的判定法であること，さらに国内で流通するウナギのアンギラ種が占める割合が高くなっていることが確認された。 It becomes the social problem of the food safety that the issue of camouflage about place of production and species in foods occurs in sequence. There are many camouflages about place of production in eel processed foods. The exhaustion of food resources of the eel has been more serious. It was conducted species identification of the raw materials of the eel processed foods using a polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method in practice of our department (food hygienics and food science experiments). The eleven samples of eel processed foods were obtained at supermarkets and restaurants in Satsuma-Sendai city. Four samples were identified as Japanese eel (Anguilla japonica), and five samples were identified as European eel (Anguilla anguilla), and two samples were not possible to identify the species. All three samples produced in China were Anguilla anguilla. In three samples produced in Japan, two samples were Anguilla japonica and one sample was not possible to identify the species. It was confirmed that it was possible to identify species of raw materials of the eel processed foods by the technique of student experiment level. Furthermore, it was confirmed that there was the deflection in species of cultivated eel (Anguilla anguilla and Anguilla japonica) between Japan and China. departmental bulletin paper