Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna
The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly unde...
Published in: | Frontiers in Plant Science |
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Language: | English |
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2020
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ftjyvaeskylaenun:oai:jyx.jyu.fi:123456789/69756 2024-05-19T07:33:16+00:00 Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna Given, Cindy Häikiö, Elina Kumar, Manoj Nissinen, Riitta 2020 application/pdf fulltext http://urn.fi/URN:NBN:fi:jyu-202006084014 eng eng Frontiers Media Frontiers in Plant Science 1664-462X 11 259180 10.3389/fpls.2020.00561 Research Council of Finland Suomen Akatemia Given, C., Häikiö, E., Kumar, M., & Nissinen, R. (2020). Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna. Frontiers in Plant Science , 11 , Article 561. https://doi.org/10.3389/fpls.2020.00561 CONVID_35849310 URN:NBN:fi:jyu-202006084014 http://urn.fi/URN:NBN:fi:jyu-202006084014 CC BY 4.0 © 2020 the Author(s) openAccess https://creativecommons.org/licenses/by/4.0/ hapro endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria bakteerit arktinen alue mikrobisto tatarkasvit endofyytit article http://purl.org/eprint/type/JournalArticle http://purl.org/coar/resource_type/c_2df8fbb1 publishedVersion A1 2020 ftjyvaeskylaenun 2024-04-23T23:38:28Z The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this ... Article in Journal/Newspaper Arctic Arctic Arktinen alue Oxyria digyna JYX - Jyväskylä University Digital Archive Frontiers in Plant Science 11 |
institution |
Open Polar |
collection |
JYX - Jyväskylä University Digital Archive |
op_collection_id |
ftjyvaeskylaenun |
language |
English |
topic |
hapro endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria bakteerit arktinen alue mikrobisto tatarkasvit endofyytit |
spellingShingle |
hapro endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria bakteerit arktinen alue mikrobisto tatarkasvit endofyytit Given, Cindy Häikiö, Elina Kumar, Manoj Nissinen, Riitta Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
topic_facet |
hapro endophytic bacteria Oxyria digyna tissue-specificity bacterial succession pioneer plant arctic bacteria bakteerit arktinen alue mikrobisto tatarkasvit endofyytit |
description |
The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this ... |
format |
Article in Journal/Newspaper |
author |
Given, Cindy Häikiö, Elina Kumar, Manoj Nissinen, Riitta |
author_facet |
Given, Cindy Häikiö, Elina Kumar, Manoj Nissinen, Riitta |
author_sort |
Given, Cindy |
title |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_short |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_full |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_fullStr |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_full_unstemmed |
Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna |
title_sort |
tissue-specific dynamics in the endophytic bacterial communities in arctic pioneer plant oxyria digyna |
publisher |
Frontiers Media |
publishDate |
2020 |
url |
http://urn.fi/URN:NBN:fi:jyu-202006084014 |
genre |
Arctic Arctic Arktinen alue Oxyria digyna |
genre_facet |
Arctic Arctic Arktinen alue Oxyria digyna |
op_relation |
Frontiers in Plant Science 1664-462X 11 259180 10.3389/fpls.2020.00561 Research Council of Finland Suomen Akatemia Given, C., Häikiö, E., Kumar, M., & Nissinen, R. (2020). Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna. Frontiers in Plant Science , 11 , Article 561. https://doi.org/10.3389/fpls.2020.00561 CONVID_35849310 URN:NBN:fi:jyu-202006084014 http://urn.fi/URN:NBN:fi:jyu-202006084014 |
op_rights |
CC BY 4.0 © 2020 the Author(s) openAccess https://creativecommons.org/licenses/by/4.0/ |
container_title |
Frontiers in Plant Science |
container_volume |
11 |
_version_ |
1799471340779995136 |