Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa)
In order to understand potential co-evolution between a parasite and its host, this study was conducted to investigate whether the apicomplexan parasite Selenidium pygospionis, specifically infects the polychaete worm, Pygospio elegans or if it has a broader distribution in other polychaete species....
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ftjyvaeskylaenun:oai:jyx.jyu.fi:123456789/64978 2023-05-15T16:51:46+02:00 Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa) Alale, Theophilus 2019 47 application/pdf fulltext http://urn.fi/URN:NBN:fi:jyu-201907043568 en eng URN:NBN:fi:jyu-201907043568 http://urn.fi/URN:NBN:fi:jyu-201907043568 Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. Tekijä ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyväskylän yliopiston kirjaston arkistotyösemalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat. The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyväskylä University Library (https://kirjasto.jyu.fi/en/workspaces/facilities). Co-evolution COX-I Marine gregarines DNA barcoding ddPCR loiset parasitismi DNA-viivakoodit isäntälajit DNA-analyysi parasites parasitism DNA barcodes host species DNA analysis Pro gradu -tutkielma Master’s thesis masterThesis G2 2019 ftjyvaeskylaenun 2023-04-12T23:04:47Z In order to understand potential co-evolution between a parasite and its host, this study was conducted to investigate whether the apicomplexan parasite Selenidium pygospionis, specifically infects the polychaete worm, Pygospio elegans or if it has a broader distribution in other polychaete species. In addition, I analyzed the diversity of the mitochondrial cytochrome c oxidase subunit I (COX-I) gene in both the host and parasite from different populations in Europe. Species specific primers were used in ddPCR to identify the presence of S. pygospionis in polychaete species obtained from Finland, Scotland, Denmark and Iceland. With additional species specific primer pairs, ABI Sanger sequencing method was used to generate 548 bp sequences from the apicomplexan COX-I gene. Genetic diversity in COX-I was compared to that determined from DNA sequence data from P. elegans in order to compare the genetic diversity between the host (P. elegans) and the parasite (S. pygospionis). The ddPCR assay suggested that Marenzelleria species had higher levels of amplified apicomplexan COX-I compared to other species sampled. However, sequences obtained from the amplicon did not verify the result. The genetic diversity for both species was low as was the interpopulation and intrapopulation genetic distances. These results indicate that S. pygospionis is a specific parasite of Pygospio elegans despite the fact that other species are likely exposed to the parasite’s oocysts in the environment. Moreover, the COX-I gene may not be sufficient to separate different Selenidium species. It will therefore require that other coding genes such as Cytochrome b (Cytb) and COX-III be tried before conclusive inference. Master Thesis Iceland JYX - Jyväskylä University Digital Archive |
institution |
Open Polar |
collection |
JYX - Jyväskylä University Digital Archive |
op_collection_id |
ftjyvaeskylaenun |
language |
English |
topic |
Co-evolution COX-I Marine gregarines DNA barcoding ddPCR loiset parasitismi DNA-viivakoodit isäntälajit DNA-analyysi parasites parasitism DNA barcodes host species DNA analysis |
spellingShingle |
Co-evolution COX-I Marine gregarines DNA barcoding ddPCR loiset parasitismi DNA-viivakoodit isäntälajit DNA-analyysi parasites parasitism DNA barcodes host species DNA analysis Alale, Theophilus Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa) |
topic_facet |
Co-evolution COX-I Marine gregarines DNA barcoding ddPCR loiset parasitismi DNA-viivakoodit isäntälajit DNA-analyysi parasites parasitism DNA barcodes host species DNA analysis |
description |
In order to understand potential co-evolution between a parasite and its host, this study was conducted to investigate whether the apicomplexan parasite Selenidium pygospionis, specifically infects the polychaete worm, Pygospio elegans or if it has a broader distribution in other polychaete species. In addition, I analyzed the diversity of the mitochondrial cytochrome c oxidase subunit I (COX-I) gene in both the host and parasite from different populations in Europe. Species specific primers were used in ddPCR to identify the presence of S. pygospionis in polychaete species obtained from Finland, Scotland, Denmark and Iceland. With additional species specific primer pairs, ABI Sanger sequencing method was used to generate 548 bp sequences from the apicomplexan COX-I gene. Genetic diversity in COX-I was compared to that determined from DNA sequence data from P. elegans in order to compare the genetic diversity between the host (P. elegans) and the parasite (S. pygospionis). The ddPCR assay suggested that Marenzelleria species had higher levels of amplified apicomplexan COX-I compared to other species sampled. However, sequences obtained from the amplicon did not verify the result. The genetic diversity for both species was low as was the interpopulation and intrapopulation genetic distances. These results indicate that S. pygospionis is a specific parasite of Pygospio elegans despite the fact that other species are likely exposed to the parasite’s oocysts in the environment. Moreover, the COX-I gene may not be sufficient to separate different Selenidium species. It will therefore require that other coding genes such as Cytochrome b (Cytb) and COX-III be tried before conclusive inference. |
format |
Master Thesis |
author |
Alale, Theophilus |
author_facet |
Alale, Theophilus |
author_sort |
Alale, Theophilus |
title |
Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa) |
title_short |
Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa) |
title_full |
Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa) |
title_fullStr |
Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa) |
title_full_unstemmed |
Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa) |
title_sort |
host specificity of selenidium pygospionis (archigregarine apicomplexa) |
publishDate |
2019 |
url |
http://urn.fi/URN:NBN:fi:jyu-201907043568 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
URN:NBN:fi:jyu-201907043568 http://urn.fi/URN:NBN:fi:jyu-201907043568 |
op_rights |
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty. This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited. Tekijä ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyväskylän yliopiston kirjaston arkistotyösemalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat. The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyväskylä University Library (https://kirjasto.jyu.fi/en/workspaces/facilities). |
_version_ |
1766041867340742656 |