Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna
Plant microbiomes consist of diverse communities of microorganisms, among which bacteria are highly abundant. The microbiomes are crucial for plants as they rely on their microbial associates for many essential functions. The goal of this thesis was to study the functional diversity and assembly rul...
Published in: | Frontiers in Plant Science |
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Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
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Jyväskylän yliopisto
2019
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ftjyvaeskylaenun:oai:jyx.jyu.fi:123456789/62995 2024-02-11T09:59:35+01:00 Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna Given, Cindy Jittrapan 2019 application/pdf fulltext http://urn.fi/URN:ISBN:978-951-39-7690-3 eng eng Jyväskylän yliopisto JYU dissertations Artikkeli I: Given, Cindy; Häikiö, Elina; Kumar, Manoj; Nissinen, Riitta (2020). Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna. Frontiers in Plant Science, 11, 561. DOI:10.3389/fpls.2020.00561 Artikkeli II: Given C.J., Häikiö E. & Nissinen R. (2018) The functional diversity of tissue- and plant type-specific endophytic bacterial community of arctic plant, Oxyria digyna. Manuscript. Artikkeli III: Given C.J., Häikiö E. & Nissinen R. (2018) The factors determining the assembly of tissue-specific endophytic bacterial communities in Oxyria digyna. Manuscript. Artikkeli IV: Given C.J. & Nissinen R. (2018) Inoculation with endophytic bacteria confers heat stress tolerance to Oxyria digyna. Manuscript. 2489-9003 62 978-951-39-7690-3 URN:ISBN:978-951-39-7690-3 http://urn.fi/URN:ISBN:978-951-39-7690-3 In Copyright © The Author & University of Jyväskylä openAccess http://rightsstatements.org/page/InC/1.0/?language=en arctic endophytic bacteria functioning holobionts micropropagated plants Oxyria digyna tissue-specificity Diss. doctoralThesis 2019 ftjyvaeskylaenun https://doi.org/10.3389/fpls.2020.00561 2024-01-25T00:02:25Z Plant microbiomes consist of diverse communities of microorganisms, among which bacteria are highly abundant. The microbiomes are crucial for plants as they rely on their microbial associates for many essential functions. The goal of this thesis was to study the functional diversity and assembly rules of endophytic bacterial communities in different plant tissues of the arcto-alpine pioneer plant species, Oxyria digyna. I used high-throughput sequencing and bacterial isolations to characterize the endophytic communities in the leaves and roots of native O. digyna plants (wild plants) and micropropagated aseptic plants (bait plants) in the field. Wild plants and tissue-propagated bait plants were shown to harbor endophytic communities with taxonomically similar structures, but with divergent functional profiles. Several plant-associated microbial traits, including nitrogen fixation and phosphate solubilization, correlated with the plant type, as did also the temperature optima of the endophytic isolates. To study endophyte community assembly, I inoculated bait plants via either leaves or roots with bacterial consortia specific for leaves or roots of O. digyna. The assembly of endophytic communities in different tissues was primarily limited by the adaptation to plant niche in the leaves, and colonization ability and competitiveness in the roots. Plant inoculation with bacterial consortia originating from different tissues (leaves or roots) restructured the innate endophytic communities, and had divergent impact on the plant phenotype. The observed differences in the plant phenotype and fitness could be explained by direct impact of inoculated bacteria on the plant metabolism (plant-microbe interaction) or indirect impact via altered functioning of the innate endophyte community (microbe-microbe interactions). Taken together, the findings in this thesis demonstrate that the endophytic bacterial communities are tissue-specific and tightly associated with their host plant, but at the same time, are highly dynamic, ... Doctoral or Postdoctoral Thesis Arctic Arctic Oxyria digyna JYX - Jyväskylä University Digital Archive Arctic Frontiers in Plant Science 11 |
institution |
Open Polar |
collection |
JYX - Jyväskylä University Digital Archive |
op_collection_id |
ftjyvaeskylaenun |
language |
English |
topic |
arctic endophytic bacteria functioning holobionts micropropagated plants Oxyria digyna tissue-specificity |
spellingShingle |
arctic endophytic bacteria functioning holobionts micropropagated plants Oxyria digyna tissue-specificity Given, Cindy Jittrapan Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna |
topic_facet |
arctic endophytic bacteria functioning holobionts micropropagated plants Oxyria digyna tissue-specificity |
description |
Plant microbiomes consist of diverse communities of microorganisms, among which bacteria are highly abundant. The microbiomes are crucial for plants as they rely on their microbial associates for many essential functions. The goal of this thesis was to study the functional diversity and assembly rules of endophytic bacterial communities in different plant tissues of the arcto-alpine pioneer plant species, Oxyria digyna. I used high-throughput sequencing and bacterial isolations to characterize the endophytic communities in the leaves and roots of native O. digyna plants (wild plants) and micropropagated aseptic plants (bait plants) in the field. Wild plants and tissue-propagated bait plants were shown to harbor endophytic communities with taxonomically similar structures, but with divergent functional profiles. Several plant-associated microbial traits, including nitrogen fixation and phosphate solubilization, correlated with the plant type, as did also the temperature optima of the endophytic isolates. To study endophyte community assembly, I inoculated bait plants via either leaves or roots with bacterial consortia specific for leaves or roots of O. digyna. The assembly of endophytic communities in different tissues was primarily limited by the adaptation to plant niche in the leaves, and colonization ability and competitiveness in the roots. Plant inoculation with bacterial consortia originating from different tissues (leaves or roots) restructured the innate endophytic communities, and had divergent impact on the plant phenotype. The observed differences in the plant phenotype and fitness could be explained by direct impact of inoculated bacteria on the plant metabolism (plant-microbe interaction) or indirect impact via altered functioning of the innate endophyte community (microbe-microbe interactions). Taken together, the findings in this thesis demonstrate that the endophytic bacterial communities are tissue-specific and tightly associated with their host plant, but at the same time, are highly dynamic, ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Given, Cindy Jittrapan |
author_facet |
Given, Cindy Jittrapan |
author_sort |
Given, Cindy Jittrapan |
title |
Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna |
title_short |
Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna |
title_full |
Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna |
title_fullStr |
Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna |
title_full_unstemmed |
Assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant Oxyria digyna |
title_sort |
assembly and functioning of endophytic bacterial communities in arcto-alpine pioneer plant oxyria digyna |
publisher |
Jyväskylän yliopisto |
publishDate |
2019 |
url |
http://urn.fi/URN:ISBN:978-951-39-7690-3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Oxyria digyna |
genre_facet |
Arctic Arctic Oxyria digyna |
op_relation |
JYU dissertations Artikkeli I: Given, Cindy; Häikiö, Elina; Kumar, Manoj; Nissinen, Riitta (2020). Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna. Frontiers in Plant Science, 11, 561. DOI:10.3389/fpls.2020.00561 Artikkeli II: Given C.J., Häikiö E. & Nissinen R. (2018) The functional diversity of tissue- and plant type-specific endophytic bacterial community of arctic plant, Oxyria digyna. Manuscript. Artikkeli III: Given C.J., Häikiö E. & Nissinen R. (2018) The factors determining the assembly of tissue-specific endophytic bacterial communities in Oxyria digyna. Manuscript. Artikkeli IV: Given C.J. & Nissinen R. (2018) Inoculation with endophytic bacteria confers heat stress tolerance to Oxyria digyna. Manuscript. 2489-9003 62 978-951-39-7690-3 URN:ISBN:978-951-39-7690-3 http://urn.fi/URN:ISBN:978-951-39-7690-3 |
op_rights |
In Copyright © The Author & University of Jyväskylä openAccess http://rightsstatements.org/page/InC/1.0/?language=en |
op_doi |
https://doi.org/10.3389/fpls.2020.00561 |
container_title |
Frontiers in Plant Science |
container_volume |
11 |
_version_ |
1790595421142253568 |