Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population
Maintaining and monitoring genetic variation in natural populations is of crucial importance for conservation and management. Reductions in genetic diversity may have short term consequences in terms of increased inbreeding depression as well as long term effects by reducing the evolutionary potenti...
Published in: | Proceedings of the 5th European Congress of Conservation Biology |
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Open Science Centre, University of Jyväskylä
2018
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ftjyvaeskylaenun:oai:jyx.jyu.fi:123456789/62277 2023-05-15T16:32:21+02:00 Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population Ekblom, Robert Persson, Jens Ellegren, Hans 2018 text/html fulltext https://doi.org/10.17011/conference/eccb2018/108056 http://urn.fi/ eng eng Open Science Centre, University of Jyväskylä https://peerageofscience.org/conference/eccb2018/108056/ ECCB2018: 5th European Congress of Conservation Biology. 12th - 15th of June 2018, Jyväskylä, Finland Ekblom, R., Persson, J. and Ellegren, H. (2018). Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population. 5th European Congress of Conservation Biology. doi:10.17011/conference/eccb2018/108056 doi:10.17011/conference/eccb2018/108056 http://urn.fi/ CC BY 4.0 © the Authors, 2018 openAccess http://creativecommons.org/licenses/by/4.0/ CC-BY Article http://purl.org/eprint/type/ConferenceItem conference paper not in proceedings publishedVersion conferenceObject 2018 ftjyvaeskylaenun https://doi.org/10.17011/conference/eccb2018/108056 2021-09-23T20:18:12Z Maintaining and monitoring genetic variation in natural populations is of crucial importance for conservation and management. Reductions in genetic diversity may have short term consequences in terms of increased inbreeding depression as well as long term effects by reducing the evolutionary potential of the population and decreasing the resilience against environmental change. With the developments in high throughput sequencing and genotyping it has now become possible to identify and monitor adaptive and neutral genetic variation at a genome-wide scale. Scandinavian wolverine (Gulo gulo) populations are subjected to habitat fragmentation and high mortality from poaching due to conflicts with livestock herders. Genetic monitoring is already an important part of the conservation programmes for this population, but the resolution and applications of genetic data have been hampered by a lack of genomic tools. Our wolverine genome assembly and re-sequencing analyses revealed a genetic diversity among the lowest ever detected in a red-listed population. Demographic analyses indicate that there has been a long-term decline of the effective population size, starting well before the last glaciation. We also found strong genome-wide signatures of inbreeding, but this effect was not observed when analysing a set of highly variable SNP markers for extended genotyping, illustrating that such markers can give a biased picture of the overall character of genetic diversity. Population genomic analyses revealed significant population structure with implications for connectivity and conservation. We used an integrated microfluidic circuit chip technology to develop an SNP-array consisting of 96 highly informative markers that, together with a multiplex pre-amplification step, was successfully applied to low-quality DNA from non-invasively collected scat samples. This work will facilitate management, conservation actions and genetic monitoring of wolverines, and also serves as a genomic roadmap that can be applied to conservation monitoring of other endangered species. peerReviewed Article in Journal/Newspaper Gulo gulo JYX - Jyväskylä University Digital Archive Proceedings of the 5th European Congress of Conservation Biology |
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Open Polar |
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JYX - Jyväskylä University Digital Archive |
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ftjyvaeskylaenun |
language |
English |
description |
Maintaining and monitoring genetic variation in natural populations is of crucial importance for conservation and management. Reductions in genetic diversity may have short term consequences in terms of increased inbreeding depression as well as long term effects by reducing the evolutionary potential of the population and decreasing the resilience against environmental change. With the developments in high throughput sequencing and genotyping it has now become possible to identify and monitor adaptive and neutral genetic variation at a genome-wide scale. Scandinavian wolverine (Gulo gulo) populations are subjected to habitat fragmentation and high mortality from poaching due to conflicts with livestock herders. Genetic monitoring is already an important part of the conservation programmes for this population, but the resolution and applications of genetic data have been hampered by a lack of genomic tools. Our wolverine genome assembly and re-sequencing analyses revealed a genetic diversity among the lowest ever detected in a red-listed population. Demographic analyses indicate that there has been a long-term decline of the effective population size, starting well before the last glaciation. We also found strong genome-wide signatures of inbreeding, but this effect was not observed when analysing a set of highly variable SNP markers for extended genotyping, illustrating that such markers can give a biased picture of the overall character of genetic diversity. Population genomic analyses revealed significant population structure with implications for connectivity and conservation. We used an integrated microfluidic circuit chip technology to develop an SNP-array consisting of 96 highly informative markers that, together with a multiplex pre-amplification step, was successfully applied to low-quality DNA from non-invasively collected scat samples. This work will facilitate management, conservation actions and genetic monitoring of wolverines, and also serves as a genomic roadmap that can be applied to conservation monitoring of other endangered species. peerReviewed |
format |
Article in Journal/Newspaper |
author |
Ekblom, Robert Persson, Jens Ellegren, Hans |
spellingShingle |
Ekblom, Robert Persson, Jens Ellegren, Hans Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population |
author_facet |
Ekblom, Robert Persson, Jens Ellegren, Hans |
author_sort |
Ekblom, Robert |
title |
Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population |
title_short |
Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population |
title_full |
Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population |
title_fullStr |
Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population |
title_full_unstemmed |
Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population |
title_sort |
genome sequencing and snp genotyping for the conservation and management of the scandinavian wolverine population |
publisher |
Open Science Centre, University of Jyväskylä |
publishDate |
2018 |
url |
https://doi.org/10.17011/conference/eccb2018/108056 http://urn.fi/ |
genre |
Gulo gulo |
genre_facet |
Gulo gulo |
op_relation |
https://peerageofscience.org/conference/eccb2018/108056/ ECCB2018: 5th European Congress of Conservation Biology. 12th - 15th of June 2018, Jyväskylä, Finland Ekblom, R., Persson, J. and Ellegren, H. (2018). Genome sequencing and SNP genotyping for the conservation and management of the Scandinavian wolverine population. 5th European Congress of Conservation Biology. doi:10.17011/conference/eccb2018/108056 doi:10.17011/conference/eccb2018/108056 http://urn.fi/ |
op_rights |
CC BY 4.0 © the Authors, 2018 openAccess http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.17011/conference/eccb2018/108056 |
container_title |
Proceedings of the 5th European Congress of Conservation Biology |
_version_ |
1766022103128080384 |