The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses
Biological nitrogen fxation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fxing and methane (CH4) oxidizing bacteria. The inhibitory efect of oxygen on microbial nitrogen fxation is documented for many...
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ftjyvaeskylaenun:oai:jyx.jyu.fi:123456789/58310 2024-02-04T10:04:51+01:00 The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses Kox, Martine A. R. Aalto, Sanni L. Penttilä, Timo Ettwig, Katharina F. Jetten, Mike S. M. van Kessel, Maartje A. H. J. 2018 application/pdf fulltext http://urn.fi/URN:NBN:fi:jyu-201805152606 eng eng Springer AMB Express 2191-0855 0 8 615146 info:eu-repo/grantAgreement/EC/FP7/615146/EU// 10.1186/s13568-018-0607-2 Euroopan komissio European Commission Kox, M. A. R., Aalto, S. L., Penttilä, T., Ettwig, K. F., Jetten, M. S. M., & van Kessel, M. A. H. J. (2018). The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses. AMB Express , 8 , Article 76. https://doi.org/10.1186/s13568-018-0607-2 CONVID_28050091 TUTKAID_77624 URN:NBN:fi:jyu-201805152606 http://urn.fi/URN:NBN:fi:jyu-201805152606 CC BY 4.0 © The Author(s) 2018. openAccess https://creativecommons.org/licenses/by/4.0/ diazotrophy methane oxidation peatland Sphagnum moss rRNA amplicon sequencing happi article http://purl.org/eprint/type/JournalArticle http://purl.org/coar/resource_type/c_2df8fbb1 publishedVersion A1 2018 ftjyvaeskylaenun 2024-01-11T00:02:29Z Biological nitrogen fxation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fxing and methane (CH4) oxidizing bacteria. The inhibitory efect of oxygen on microbial nitrogen fxation is documented for many bacteria. However, the role of nitrogenfxing methanotrophs in nitrogen supply to Sphagnum peat mosses is not well explored. Here, we investigated the role of both oxygen and methane on nitrogen fxation in subarctic Sphagnum peat mosses. Five species of Sphagnum mosses were sampled from two mesotrophic and three oligotrophic sites within the Lakkasuo peatland in Orivesi, central Finland. Mosses were incubated under either ambient or low oxygen conditions in the presence or absence of methane. Stable isotope activity assays revealed considerable nitrogen-fxing and methane-assimilating rates at all sites (1.4±0.2 µmol 15N–N2 g−1 DW day−1 and 12.0±1.1 µmol 13C–CH4 g−1 DW day−1 , respectively). Addition of methane did not stimulate incorporation of 15N-nitrogen into biomass, whereas oxygen depletion increased the activity of the nitrogen-fxing community. Analysis of the 16S rRNA genes at the bacterial community level showed a very diverse microbiome that was dominated by Alphaproteobacteria in all sites. Bona fde methane-oxidizing taxa were not very abundant (relative abundance less than 0.1%). Based on our results we conclude that methanotrophs did not contribute signifcantly to nitrogen fxation in the investigated peatlands. peerReviewed Article in Journal/Newspaper Subarctic JYX - Jyväskylä University Digital Archive |
institution |
Open Polar |
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JYX - Jyväskylä University Digital Archive |
op_collection_id |
ftjyvaeskylaenun |
language |
English |
topic |
diazotrophy methane oxidation peatland Sphagnum moss rRNA amplicon sequencing happi |
spellingShingle |
diazotrophy methane oxidation peatland Sphagnum moss rRNA amplicon sequencing happi Kox, Martine A. R. Aalto, Sanni L. Penttilä, Timo Ettwig, Katharina F. Jetten, Mike S. M. van Kessel, Maartje A. H. J. The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
topic_facet |
diazotrophy methane oxidation peatland Sphagnum moss rRNA amplicon sequencing happi |
description |
Biological nitrogen fxation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fxing and methane (CH4) oxidizing bacteria. The inhibitory efect of oxygen on microbial nitrogen fxation is documented for many bacteria. However, the role of nitrogenfxing methanotrophs in nitrogen supply to Sphagnum peat mosses is not well explored. Here, we investigated the role of both oxygen and methane on nitrogen fxation in subarctic Sphagnum peat mosses. Five species of Sphagnum mosses were sampled from two mesotrophic and three oligotrophic sites within the Lakkasuo peatland in Orivesi, central Finland. Mosses were incubated under either ambient or low oxygen conditions in the presence or absence of methane. Stable isotope activity assays revealed considerable nitrogen-fxing and methane-assimilating rates at all sites (1.4±0.2 µmol 15N–N2 g−1 DW day−1 and 12.0±1.1 µmol 13C–CH4 g−1 DW day−1 , respectively). Addition of methane did not stimulate incorporation of 15N-nitrogen into biomass, whereas oxygen depletion increased the activity of the nitrogen-fxing community. Analysis of the 16S rRNA genes at the bacterial community level showed a very diverse microbiome that was dominated by Alphaproteobacteria in all sites. Bona fde methane-oxidizing taxa were not very abundant (relative abundance less than 0.1%). Based on our results we conclude that methanotrophs did not contribute signifcantly to nitrogen fxation in the investigated peatlands. peerReviewed |
format |
Article in Journal/Newspaper |
author |
Kox, Martine A. R. Aalto, Sanni L. Penttilä, Timo Ettwig, Katharina F. Jetten, Mike S. M. van Kessel, Maartje A. H. J. |
author_facet |
Kox, Martine A. R. Aalto, Sanni L. Penttilä, Timo Ettwig, Katharina F. Jetten, Mike S. M. van Kessel, Maartje A. H. J. |
author_sort |
Kox, Martine A. R. |
title |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_short |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_full |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_fullStr |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_full_unstemmed |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_sort |
influence of oxygen and methane on nitrogen fixation in subarctic sphagnum mosses |
publisher |
Springer |
publishDate |
2018 |
url |
http://urn.fi/URN:NBN:fi:jyu-201805152606 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
AMB Express 2191-0855 0 8 615146 info:eu-repo/grantAgreement/EC/FP7/615146/EU// 10.1186/s13568-018-0607-2 Euroopan komissio European Commission Kox, M. A. R., Aalto, S. L., Penttilä, T., Ettwig, K. F., Jetten, M. S. M., & van Kessel, M. A. H. J. (2018). The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses. AMB Express , 8 , Article 76. https://doi.org/10.1186/s13568-018-0607-2 CONVID_28050091 TUTKAID_77624 URN:NBN:fi:jyu-201805152606 http://urn.fi/URN:NBN:fi:jyu-201805152606 |
op_rights |
CC BY 4.0 © The Author(s) 2018. openAccess https://creativecommons.org/licenses/by/4.0/ |
_version_ |
1789973587953713152 |