PHENOTYPIC AND GENOTYPIC ANALYSIS OF AN ARCANOBACTERIUM PLURANIMALIUM ISOLATED FROM A MUSKOX (OVIBOS MOSCHATUS)

• Main Authors: Ningrum, Siti Gusti, Arnafia, Wyanda, Sammra, Osama, Alssahen, Mazen, Wickhorst, Jörn-Peter, Hassan, Abdulwahed Ahmed, Lämmler, Christoph, Prenger-Berninghoff, Ellen, Timke, Markus, Abdulmawjood, Amir
• Other Authors: Justus-Liebig-Universität, University of Mosul, Bruker Daltonik GmbH, Stiftung Tierärztliche Hochschule Hannover
• Format: Article in Journal/Newspaper
• Language: English
• Published: Veterinarski fakultet Sarajevo 2017
• Subjects: muskox; ovibos moschatus
• Online Access: https://www.veterinaria-sarajevo.com/ojs/index.php/vet/article/view/261

The present study was designed to characterize an Arcanobacterium pluranimalium strain isolated from a muskox (Ovibos moschatus) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S rRNA gene, the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf and the pluranimaliumlysin encoding gene pla allowed a successful identification of the isolated strain as A. pluranimalium. Gene pla could also be detected by a previously described loop-mediated isothermal amplification (LAMP) assay. This is first report on the isolation and characterization of A. pluranimalium originated from a Muskox.