Molecular-mass distribution of humus acids of taiga soils
Purpose of the study: to reveal the influence of hydromorphism and agricultural use on the molecular-mass distribution (MMD) of humic (HAs) and fulvic acids (FAs) isolated from the soils of the European northeast of Russia. To assess the influence of the acidity of the medium on the MWD of FAs. Plac...
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ФГБУН Институт почвоведения и агрохимии СО РАН
2021
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ftjsoils:oai:https://soils-journal.ru/index.php/POS/oai:article/160 2023-05-15T18:30:35+02:00 Molecular-mass distribution of humus acids of taiga soils Молекулярно-массовое распределение гуминовых и фульвокислот таёжных почв Лодыгин, Евгений Дмитриевич Василевич, Роман Сергеевич 2021-12-30 application/pdf https://soils-journal.ru/index.php/POS/article/view/160 rus rus ФГБУН Институт почвоведения и агрохимии СО РАН https://soils-journal.ru/index.php/POS/article/view/160/223 https://soils-journal.ru/index.php/POS/article/view/160 Copyright (c) 2021 Почвы и окружающая среда https://creativecommons.org/licenses/by/4.0/ CC-BY The Journal of Soils and Environment; Vol. 4 No. 4 (2021): The Journal of Soils and Environment; e160 Почвы и окружающая среда; Том 4 № 4 (2021): Почвы и окружающая среда; e160 2618-6802 гуминовые кислоты фульвокислоты полидисперстность эксклюзионная хроматография подзолистые болотно-подзолистые пахотные почвы humic acids fulvic acids polydispersity size exclusion chromatography podzolic bog-podzolic arable soil info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reviewed article Original article Рецензируемая статья Оригинальная статья 2021 ftjsoils 2022-12-20T17:41:29Z Purpose of the study: to reveal the influence of hydromorphism and agricultural use on the molecular-mass distribution (MMD) of humic (HAs) and fulvic acids (FAs) isolated from the soils of the European northeast of Russia. To assess the influence of the acidity of the medium on the MWD of FAs. Place and time of the event. The studies were carried out on the territory of the middle taiga (Maksimovsky station of the Institute of Biology of the Federal Research Center of the KSC UB RAS, located 8 km west of the city of Syktyvkar and the field of the Syktyvkar state farm, 5 km south-west of Syktyvkar, on the watershed of the Sysola and Vazhel-yu rivers) and northern taiga (3 km west of the Troitsko-Pechorsk station). The objects of the study were humic substances isolated from typical podzolic (Eutric Albic Retisol (Loamic)), gleypodzolic (Eutric Albic Stagnic Retisol (Loamic)), peaty-podzolic surface-gleyed (Eutric Albic Stagnic Histic) Retisol (Loamic Eutric Albic Retisol (Loamic)) soils. The soil sampling period is from 1 to 30 August 2014. Methodology. Quantitative analysis of the MMD of HA and FA preparations was performed using liquid size exclusion chromatography (gel chromatography) on Sephadex G-25 and G-100 gels with continuous automatic registration of the optical density of the eluate (λ = 280 nm) in a quartz flow cell, K 9 × 60 cm column (Pharmacia, Sweden). Distilled water, 0.05 M NaOH solution, and Tris-HCl buffer with pH = 8.2 were used as the eluent. Main results. Studies of the MMD of humic substances have been carried out. It was found that HAs contain three fractions with different molecular weights: ≥ 150 kDa, 73–80 kDa, and 13–23 kDa; FAs – two fractions: ≥ 5 kDa, 1–2 kDa. The low molecular weight of FAs, together with a large amount of oxygen-containing functional groups, contributes to their better solubility and migration ability in soils. The HAs of automorphic soils are characterized by a high content of low molecular weight fractions, and the proportion of the high molecular weight ... Article in Journal/Newspaper taiga The Journal of Soils and Environment |
institution |
Open Polar |
collection |
The Journal of Soils and Environment |
op_collection_id |
ftjsoils |
language |
Russian |
topic |
гуминовые кислоты фульвокислоты полидисперстность эксклюзионная хроматография подзолистые болотно-подзолистые пахотные почвы humic acids fulvic acids polydispersity size exclusion chromatography podzolic bog-podzolic arable soil |
spellingShingle |
гуминовые кислоты фульвокислоты полидисперстность эксклюзионная хроматография подзолистые болотно-подзолистые пахотные почвы humic acids fulvic acids polydispersity size exclusion chromatography podzolic bog-podzolic arable soil Лодыгин, Евгений Дмитриевич Василевич, Роман Сергеевич Molecular-mass distribution of humus acids of taiga soils |
topic_facet |
гуминовые кислоты фульвокислоты полидисперстность эксклюзионная хроматография подзолистые болотно-подзолистые пахотные почвы humic acids fulvic acids polydispersity size exclusion chromatography podzolic bog-podzolic arable soil |
description |
Purpose of the study: to reveal the influence of hydromorphism and agricultural use on the molecular-mass distribution (MMD) of humic (HAs) and fulvic acids (FAs) isolated from the soils of the European northeast of Russia. To assess the influence of the acidity of the medium on the MWD of FAs. Place and time of the event. The studies were carried out on the territory of the middle taiga (Maksimovsky station of the Institute of Biology of the Federal Research Center of the KSC UB RAS, located 8 km west of the city of Syktyvkar and the field of the Syktyvkar state farm, 5 km south-west of Syktyvkar, on the watershed of the Sysola and Vazhel-yu rivers) and northern taiga (3 km west of the Troitsko-Pechorsk station). The objects of the study were humic substances isolated from typical podzolic (Eutric Albic Retisol (Loamic)), gleypodzolic (Eutric Albic Stagnic Retisol (Loamic)), peaty-podzolic surface-gleyed (Eutric Albic Stagnic Histic) Retisol (Loamic Eutric Albic Retisol (Loamic)) soils. The soil sampling period is from 1 to 30 August 2014. Methodology. Quantitative analysis of the MMD of HA and FA preparations was performed using liquid size exclusion chromatography (gel chromatography) on Sephadex G-25 and G-100 gels with continuous automatic registration of the optical density of the eluate (λ = 280 nm) in a quartz flow cell, K 9 × 60 cm column (Pharmacia, Sweden). Distilled water, 0.05 M NaOH solution, and Tris-HCl buffer with pH = 8.2 were used as the eluent. Main results. Studies of the MMD of humic substances have been carried out. It was found that HAs contain three fractions with different molecular weights: ≥ 150 kDa, 73–80 kDa, and 13–23 kDa; FAs – two fractions: ≥ 5 kDa, 1–2 kDa. The low molecular weight of FAs, together with a large amount of oxygen-containing functional groups, contributes to their better solubility and migration ability in soils. The HAs of automorphic soils are characterized by a high content of low molecular weight fractions, and the proportion of the high molecular weight ... |
format |
Article in Journal/Newspaper |
author |
Лодыгин, Евгений Дмитриевич Василевич, Роман Сергеевич |
author_facet |
Лодыгин, Евгений Дмитриевич Василевич, Роман Сергеевич |
author_sort |
Лодыгин, Евгений Дмитриевич |
title |
Molecular-mass distribution of humus acids of taiga soils |
title_short |
Molecular-mass distribution of humus acids of taiga soils |
title_full |
Molecular-mass distribution of humus acids of taiga soils |
title_fullStr |
Molecular-mass distribution of humus acids of taiga soils |
title_full_unstemmed |
Molecular-mass distribution of humus acids of taiga soils |
title_sort |
molecular-mass distribution of humus acids of taiga soils |
publisher |
ФГБУН Институт почвоведения и агрохимии СО РАН |
publishDate |
2021 |
url |
https://soils-journal.ru/index.php/POS/article/view/160 |
genre |
taiga |
genre_facet |
taiga |
op_source |
The Journal of Soils and Environment; Vol. 4 No. 4 (2021): The Journal of Soils and Environment; e160 Почвы и окружающая среда; Том 4 № 4 (2021): Почвы и окружающая среда; e160 2618-6802 |
op_relation |
https://soils-journal.ru/index.php/POS/article/view/160/223 https://soils-journal.ru/index.php/POS/article/view/160 |
op_rights |
Copyright (c) 2021 Почвы и окружающая среда https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
_version_ |
1766214122844717056 |