Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica
ABSTRACT Antarctica harbors a great diversity of microorganisms, including bacteria, archaea, microalgae and yeasts. The Pseudomonas genus is one of the most diverse and successful bacterial groups described to date, but only eight species isolated from Antarctica have been characterized. Here, we p...
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ftjscielo:oai:scielo:S1517-83822018000400695 2023-05-15T13:51:18+02:00 Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica Vásquez-Ponce,Felipe Higuera-Llantén,Sebastián Pavlov,María S. Marshall,Sergio H. Olivares-Pacheco,Jorge 2018-12-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400695 en eng Sociedade Brasileira de Microbiologia 10.1016/j.bjm.2018.02.005 info:eu-repo/semantics/openAccess Brazilian Journal of Microbiology v.49 n.4 2018 Pseudomonas Antarctica P. fluorescens complex Multilocus Sequence Analysis info:eu-repo/semantics/article 2018 ftjscielo 2019-01-06T06:52:08Z ABSTRACT Antarctica harbors a great diversity of microorganisms, including bacteria, archaea, microalgae and yeasts. The Pseudomonas genus is one of the most diverse and successful bacterial groups described to date, but only eight species isolated from Antarctica have been characterized. Here, we present three potentially novel species isolated on King George Island. The most abundant isolates from four different environments, were genotypically and phenotypically characterized. Multilocus sequence analysis and 16S rRNA gene analysis of a sequence concatenate for six genes (16S, aroE, glnS, gyrB, ileS and rpoD), determined one of the isolates to be a new Pseudomonas mandelii strain, while the other three are good candidates for new Pseudomonas species. Additionally, genotype analyses showed the three candidates to be part of a new subgroup within the Pseudomonas fluorescens complex, together with the Antarctic species Pseudomonas antarctica and Pseudomonas extremaustralis. We propose terming this new subgroup P. antarctica. Likewise, phenotypic analyses using API 20 NE and BIOLOG® corroborated the genotyping results, confirming that all presented isolates form part of the P. fluorescens complex. Pseudomonas genus research on the Antarctic continent is in its infancy. To understand these microorganisms’ role in this extreme environment, the characterization and description of new species is vital. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island SciELO Brazil (Scientific Electronic Library Online) Antarctic The Antarctic King George Island |
institution |
Open Polar |
collection |
SciELO Brazil (Scientific Electronic Library Online) |
op_collection_id |
ftjscielo |
language |
English |
topic |
Pseudomonas Antarctica P. fluorescens complex Multilocus Sequence Analysis |
spellingShingle |
Pseudomonas Antarctica P. fluorescens complex Multilocus Sequence Analysis Vásquez-Ponce,Felipe Higuera-Llantén,Sebastián Pavlov,María S. Marshall,Sergio H. Olivares-Pacheco,Jorge Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica |
topic_facet |
Pseudomonas Antarctica P. fluorescens complex Multilocus Sequence Analysis |
description |
ABSTRACT Antarctica harbors a great diversity of microorganisms, including bacteria, archaea, microalgae and yeasts. The Pseudomonas genus is one of the most diverse and successful bacterial groups described to date, but only eight species isolated from Antarctica have been characterized. Here, we present three potentially novel species isolated on King George Island. The most abundant isolates from four different environments, were genotypically and phenotypically characterized. Multilocus sequence analysis and 16S rRNA gene analysis of a sequence concatenate for six genes (16S, aroE, glnS, gyrB, ileS and rpoD), determined one of the isolates to be a new Pseudomonas mandelii strain, while the other three are good candidates for new Pseudomonas species. Additionally, genotype analyses showed the three candidates to be part of a new subgroup within the Pseudomonas fluorescens complex, together with the Antarctic species Pseudomonas antarctica and Pseudomonas extremaustralis. We propose terming this new subgroup P. antarctica. Likewise, phenotypic analyses using API 20 NE and BIOLOG® corroborated the genotyping results, confirming that all presented isolates form part of the P. fluorescens complex. Pseudomonas genus research on the Antarctic continent is in its infancy. To understand these microorganisms’ role in this extreme environment, the characterization and description of new species is vital. |
format |
Article in Journal/Newspaper |
author |
Vásquez-Ponce,Felipe Higuera-Llantén,Sebastián Pavlov,María S. Marshall,Sergio H. Olivares-Pacheco,Jorge |
author_facet |
Vásquez-Ponce,Felipe Higuera-Llantén,Sebastián Pavlov,María S. Marshall,Sergio H. Olivares-Pacheco,Jorge |
author_sort |
Vásquez-Ponce,Felipe |
title |
Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica |
title_short |
Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica |
title_full |
Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica |
title_fullStr |
Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica |
title_full_unstemmed |
Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica |
title_sort |
phylogenetic mlsa and phenotypic analysis identification of three probable novel pseudomonas species isolated on king george island, south shetland, antarctica |
publisher |
Sociedade Brasileira de Microbiologia |
publishDate |
2018 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400695 |
geographic |
Antarctic The Antarctic King George Island |
geographic_facet |
Antarctic The Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
Brazilian Journal of Microbiology v.49 n.4 2018 |
op_relation |
10.1016/j.bjm.2018.02.005 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766255093704818688 |