Vanadium-binding protein in marine plankton from tropical south atlantic ocean
We investigated the role of plankton in the vanadium (V) cycle at Cabo Frio, Rio de Janeiro state, Brazil, a region with high V concentration in the atmospheric particles due to marine aerosol. The concentrations of V in plankton vary from 0.08 to 20.9 µg g-1 (zooplankton), 0.1 to 28.4 µg g-1 (phyto...
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Sociedade Brasileira de Química
2014
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ftjscielo:oai:scielo:S0103-50532014000600017 2023-05-15T18:21:07+02:00 Vanadium-binding protein in marine plankton from tropical south atlantic ocean Kütter,Vinicius T. Montes-Bayón,Maria Sella,Silvia M. Sanz-Medel,Alfredo Silva-Filho,Emmanoel V. 2014-06-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600017 en eng Sociedade Brasileira de Química Journal of the Brazilian Chemical Society v.25 n.6 2014 bioaccumulation biogeochemistry chemical ecology HPLC-ICP-MS metallothionein journal article 2014 ftjscielo 2015-10-26T20:48:38Z We investigated the role of plankton in the vanadium (V) cycle at Cabo Frio, Rio de Janeiro state, Brazil, a region with high V concentration in the atmospheric particles due to marine aerosol. The concentrations of V in plankton vary from 0.08 to 20.9 µg g-1 (zooplankton), 0.1 to 28.4 µg g-1 (phytoplankton > 64 µm) and < 0.0005 to 49.0 µg g-1 (small phytoplankton > 20 µm). The V speciation in biomolecules was performed by the use of two strategies: (i) coupling of size exclusion chromatography (SEC) for the fractionation of species with inductively coupled plasma mass spectrometry (ICP-MS) and (ii) with size exclusion anionic exchange chromatography with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (SEC-AE-UV-Vis-ICP-MS). The results showed a single fraction containing V associated with a biomolecule in the range of 8 to 16 kDa, with isoeletric points above 8. The preliminary analyses using matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) do not permit to identify such biomolecule, considering the broader size range of the proteins obtained. Article in Journal/Newspaper South Atlantic Ocean SciELO Brazil (Scientific Electronic Library Online) |
institution |
Open Polar |
collection |
SciELO Brazil (Scientific Electronic Library Online) |
op_collection_id |
ftjscielo |
language |
English |
topic |
bioaccumulation biogeochemistry chemical ecology HPLC-ICP-MS metallothionein |
spellingShingle |
bioaccumulation biogeochemistry chemical ecology HPLC-ICP-MS metallothionein Kütter,Vinicius T. Montes-Bayón,Maria Sella,Silvia M. Sanz-Medel,Alfredo Silva-Filho,Emmanoel V. Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
topic_facet |
bioaccumulation biogeochemistry chemical ecology HPLC-ICP-MS metallothionein |
description |
We investigated the role of plankton in the vanadium (V) cycle at Cabo Frio, Rio de Janeiro state, Brazil, a region with high V concentration in the atmospheric particles due to marine aerosol. The concentrations of V in plankton vary from 0.08 to 20.9 µg g-1 (zooplankton), 0.1 to 28.4 µg g-1 (phytoplankton > 64 µm) and < 0.0005 to 49.0 µg g-1 (small phytoplankton > 20 µm). The V speciation in biomolecules was performed by the use of two strategies: (i) coupling of size exclusion chromatography (SEC) for the fractionation of species with inductively coupled plasma mass spectrometry (ICP-MS) and (ii) with size exclusion anionic exchange chromatography with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (SEC-AE-UV-Vis-ICP-MS). The results showed a single fraction containing V associated with a biomolecule in the range of 8 to 16 kDa, with isoeletric points above 8. The preliminary analyses using matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) do not permit to identify such biomolecule, considering the broader size range of the proteins obtained. |
format |
Article in Journal/Newspaper |
author |
Kütter,Vinicius T. Montes-Bayón,Maria Sella,Silvia M. Sanz-Medel,Alfredo Silva-Filho,Emmanoel V. |
author_facet |
Kütter,Vinicius T. Montes-Bayón,Maria Sella,Silvia M. Sanz-Medel,Alfredo Silva-Filho,Emmanoel V. |
author_sort |
Kütter,Vinicius T. |
title |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_short |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_full |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_fullStr |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_full_unstemmed |
Vanadium-binding protein in marine plankton from tropical south atlantic ocean |
title_sort |
vanadium-binding protein in marine plankton from tropical south atlantic ocean |
publisher |
Sociedade Brasileira de Química |
publishDate |
2014 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000600017 |
genre |
South Atlantic Ocean |
genre_facet |
South Atlantic Ocean |
op_source |
Journal of the Brazilian Chemical Society v.25 n.6 2014 |
_version_ |
1766200219945402368 |