Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells
Abstract Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic kr...
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ftjscielo:oai:scielo:S0101-20612021005007227 2023-05-15T13:41:51+02:00 Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells WANG,Shanshan ZHOU,Deqing LIN,Haiyan 2021-01-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021005007227 en eng Sociedade Brasileira de Ciência e Tecnologia de Alimentos 10.1590/fst.64920 info:eu-repo/semantics/openAccess Food Science and Technology n.ahead 2021 phosphorylation Antarctic krill peptides antioxidant activity oxidative damage MC3T3-E1 cells info:eu-repo/semantics/article 2021 ftjscielo 2021-03-11T11:28:10Z Abstract Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic krill peptide (AKP). In hydrogen peroxide (H2O2)-induced oxidative damage of MC3T3-E1 cells, both AKP and P-AKP pretreatment could dose-dependently improve superoxide dismutase (SOD) and catalase (CAT) activities through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production. Moreover, AKP and P-AKP prevented oxidative stress-induced down regulation of alkaline phosphatase (ALP) activity and matrix mineralization. Particularly, the promoting effects of P-AKP on the enzymatic antioxidant defense system, differentiation and mineralization was higher than that of AKP. These results suggested that phosphorylation might be a promising approach to improve the antioxidant and osteogenic activity of AKP, and P-AKP could be a beneficial agent for attenuating oxidative stress-related bone loss. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba SciELO Brazil (Scientific Electronic Library Online) Antarctic |
institution |
Open Polar |
collection |
SciELO Brazil (Scientific Electronic Library Online) |
op_collection_id |
ftjscielo |
language |
English |
topic |
phosphorylation Antarctic krill peptides antioxidant activity oxidative damage MC3T3-E1 cells |
spellingShingle |
phosphorylation Antarctic krill peptides antioxidant activity oxidative damage MC3T3-E1 cells WANG,Shanshan ZHOU,Deqing LIN,Haiyan Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
topic_facet |
phosphorylation Antarctic krill peptides antioxidant activity oxidative damage MC3T3-E1 cells |
description |
Abstract Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic krill peptide (AKP). In hydrogen peroxide (H2O2)-induced oxidative damage of MC3T3-E1 cells, both AKP and P-AKP pretreatment could dose-dependently improve superoxide dismutase (SOD) and catalase (CAT) activities through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production. Moreover, AKP and P-AKP prevented oxidative stress-induced down regulation of alkaline phosphatase (ALP) activity and matrix mineralization. Particularly, the promoting effects of P-AKP on the enzymatic antioxidant defense system, differentiation and mineralization was higher than that of AKP. These results suggested that phosphorylation might be a promising approach to improve the antioxidant and osteogenic activity of AKP, and P-AKP could be a beneficial agent for attenuating oxidative stress-related bone loss. |
format |
Article in Journal/Newspaper |
author |
WANG,Shanshan ZHOU,Deqing LIN,Haiyan |
author_facet |
WANG,Shanshan ZHOU,Deqing LIN,Haiyan |
author_sort |
WANG,Shanshan |
title |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_short |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_full |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_fullStr |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_full_unstemmed |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_sort |
ameliorative effects of phosphorylated peptide from antarctic krill (euphausia superba) against h2o2-induced damage in mc3t3-e1 cells |
publisher |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publishDate |
2021 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021005007227 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_source |
Food Science and Technology n.ahead 2021 |
op_relation |
10.1590/fst.64920 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766159136329826304 |