ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL

• Main Authors: Ficanha,Aline Matuella Moreira, Nyari,Nádia Ligianara D., Levandoski,Katarine, Mignoni,Marcelo Luis, Dallago,Rogério Marcos
• Format: Article in Journal/Newspaper
• Language: Portuguese
• Published: Sociedade Brasileira de Química 2015
• Subjects: immobilization; lipase; sol-gel; PEG 1500; esterification; Antarc*; Antarctica
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300364

The objective of this work was the immobilization of the enzyme Candida antarctica lipase B (CAL B) using the sol-gel method of immobilization and three different initiators of the polymerization reaction: one acid (HCl), one basic (NH4OH) and the other nucleophilic (HBr). Tetraethylorthosilicate was used as the silica precursor. The influence of the additive PEG 1500 on immobilization was assessed. The efficiency of the process was evaluated considering the esterification activity of the xerogels. The immobilization process provided enhanced thermal stability, storage and operational aspects relative to the free enzyme. Storage temperature proved one of the main variables to be considered in the process, with the xerogels stored under refrigeration showing better results in terms of residual activity (nearly 200 days with ≥ 90% residual activity of basic and nucleophilic xerogels) when compared with storage at ambient temperature (nearly 40 days). The results demonstrated the possibility of reuse of derivatives and a greater number of cycles (nine), considering a residual activity of 50%.