In vitro radical-scavenging activity, toxicity against A. salina, and nmr profiles of extracts of lichens collected from Brazil and Antarctica

• Main Authors: Ravaglia,Luciana M., Gonçalves,Kerolayne, Oyama,Nathália Mitsuko, Coelho,Roberta Gomes, Spielmann,Adriano A., Honda,Neli K.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Química 2014
• Subjects: lichens; phenolic compounds; NMR; radical-scavenging potential; Artemia salina; Antarc*; Antarctica
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000600016

Extracts of six lichen species collected from Brazil and Antarctica were investigated for their potential toxicity and radical-scavenging properties. The composition of the extracts was investigated using TLC and NMR, leading to identification of atranorin (1), along with salazinic (2), barbatic (3), α-alectoronic (4), α-collatolic (5), cryptochlorophaeic (6), caperatic (7), lobaric (8), and protolichesterinic (9) acids. All acetone extracts were evaluated for their 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability and subjected to Artemia salina bioassay. The free-radical-scavenging activities of each extract (100 µg) ranged from 8.9 ± 0.1% to 38.7 ± 2.5% and the EC50 values ranged from 0.24 ± 2.10 to 3.54 ± 0.28 mg mL-1, while the toxicity of the extracts against A. salina were low (151.0 to >600 µg mL-1).