Potencial de biocatálise enantiosseletiva de lipases microbianas
Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; G...
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ftjscielo:oai:scielo:S0100-40422005000400012 2023-05-15T13:46:23+02:00 Potencial de biocatálise enantiosseletiva de lipases microbianas Carvalho,Patrícia de O. Calafatti,Silvana Ap. Marassi,Maurício Silva,Daniela M. da Contesini,Fabiano J. Bizaco,Renato Macedo,Gabriela Alves 2005-08-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000400012 pt por Sociedade Brasileira de Química Química Nova v.28 n.4 2005 lipases enantioselectivity (R,S)-ibuprofen journal article 2005 ftjscielo 2015-10-26T18:11:10Z Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; Geotrichum candidum; Penicillium solitum) were compared in terms of their stability and as biocatalysts in the enantioselective esterification using racemic substrates in organic medium. The lipase from Aspergillus niger showed the highest activity (18.2 U/mL) and was highly thermostable, retaining 90% and 60% activity at 50 ºC and 60 ºC after 1 hour, respectively. In organic medium, this lipase provided the best results in terms of enantiomeric excess of the (S)-active acid (ee = 6.1%) and conversion value (c = 20%) in the esterification of (R,S)-ibuprofen with 1-propanol in isooctane. The esterification reaction of the racemic mixture of (R,S)-2-octanol with decanoic acid proceeded with high enantioselectivity when lipase from Aspergillus niger (E = 13.2) and commercial lipase from Candida antarctica (E = 20) were employed. Article in Journal/Newspaper Antarc* Antarctica SciELO Brazil (Scientific Electronic Library Online) |
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Open Polar |
collection |
SciELO Brazil (Scientific Electronic Library Online) |
op_collection_id |
ftjscielo |
language |
Portuguese |
topic |
lipases enantioselectivity (R,S)-ibuprofen |
spellingShingle |
lipases enantioselectivity (R,S)-ibuprofen Carvalho,Patrícia de O. Calafatti,Silvana Ap. Marassi,Maurício Silva,Daniela M. da Contesini,Fabiano J. Bizaco,Renato Macedo,Gabriela Alves Potencial de biocatálise enantiosseletiva de lipases microbianas |
topic_facet |
lipases enantioselectivity (R,S)-ibuprofen |
description |
Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; Geotrichum candidum; Penicillium solitum) were compared in terms of their stability and as biocatalysts in the enantioselective esterification using racemic substrates in organic medium. The lipase from Aspergillus niger showed the highest activity (18.2 U/mL) and was highly thermostable, retaining 90% and 60% activity at 50 ºC and 60 ºC after 1 hour, respectively. In organic medium, this lipase provided the best results in terms of enantiomeric excess of the (S)-active acid (ee = 6.1%) and conversion value (c = 20%) in the esterification of (R,S)-ibuprofen with 1-propanol in isooctane. The esterification reaction of the racemic mixture of (R,S)-2-octanol with decanoic acid proceeded with high enantioselectivity when lipase from Aspergillus niger (E = 13.2) and commercial lipase from Candida antarctica (E = 20) were employed. |
format |
Article in Journal/Newspaper |
author |
Carvalho,Patrícia de O. Calafatti,Silvana Ap. Marassi,Maurício Silva,Daniela M. da Contesini,Fabiano J. Bizaco,Renato Macedo,Gabriela Alves |
author_facet |
Carvalho,Patrícia de O. Calafatti,Silvana Ap. Marassi,Maurício Silva,Daniela M. da Contesini,Fabiano J. Bizaco,Renato Macedo,Gabriela Alves |
author_sort |
Carvalho,Patrícia de O. |
title |
Potencial de biocatálise enantiosseletiva de lipases microbianas |
title_short |
Potencial de biocatálise enantiosseletiva de lipases microbianas |
title_full |
Potencial de biocatálise enantiosseletiva de lipases microbianas |
title_fullStr |
Potencial de biocatálise enantiosseletiva de lipases microbianas |
title_full_unstemmed |
Potencial de biocatálise enantiosseletiva de lipases microbianas |
title_sort |
potencial de biocatálise enantiosseletiva de lipases microbianas |
publisher |
Sociedade Brasileira de Química |
publishDate |
2005 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000400012 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Química Nova v.28 n.4 2005 |
_version_ |
1766241417328328704 |