Potencial de biocatálise enantiosseletiva de lipases microbianas

Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; G...

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Main Authors: Carvalho,Patrícia de O., Calafatti,Silvana Ap., Marassi,Maurício, Silva,Daniela M. da, Contesini,Fabiano J., Bizaco,Renato, Macedo,Gabriela Alves
Format: Article in Journal/Newspaper
Language:Portuguese
Published: Sociedade Brasileira de Química 2005
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000400012
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spelling ftjscielo:oai:scielo:S0100-40422005000400012 2023-05-15T13:46:23+02:00 Potencial de biocatálise enantiosseletiva de lipases microbianas Carvalho,Patrícia de O. Calafatti,Silvana Ap. Marassi,Maurício Silva,Daniela M. da Contesini,Fabiano J. Bizaco,Renato Macedo,Gabriela Alves 2005-08-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000400012 pt por Sociedade Brasileira de Química Química Nova v.28 n.4 2005 lipases enantioselectivity (R,S)-ibuprofen journal article 2005 ftjscielo 2015-10-26T18:11:10Z Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; Geotrichum candidum; Penicillium solitum) were compared in terms of their stability and as biocatalysts in the enantioselective esterification using racemic substrates in organic medium. The lipase from Aspergillus niger showed the highest activity (18.2 U/mL) and was highly thermostable, retaining 90% and 60% activity at 50 ºC and 60 ºC after 1 hour, respectively. In organic medium, this lipase provided the best results in terms of enantiomeric excess of the (S)-active acid (ee = 6.1%) and conversion value (c = 20%) in the esterification of (R,S)-ibuprofen with 1-propanol in isooctane. The esterification reaction of the racemic mixture of (R,S)-2-octanol with decanoic acid proceeded with high enantioselectivity when lipase from Aspergillus niger (E = 13.2) and commercial lipase from Candida antarctica (E = 20) were employed. Article in Journal/Newspaper Antarc* Antarctica SciELO Brazil (Scientific Electronic Library Online)
institution Open Polar
collection SciELO Brazil (Scientific Electronic Library Online)
op_collection_id ftjscielo
language Portuguese
topic lipases
enantioselectivity
(R,S)-ibuprofen
spellingShingle lipases
enantioselectivity
(R,S)-ibuprofen
Carvalho,Patrícia de O.
Calafatti,Silvana Ap.
Marassi,Maurício
Silva,Daniela M. da
Contesini,Fabiano J.
Bizaco,Renato
Macedo,Gabriela Alves
Potencial de biocatálise enantiosseletiva de lipases microbianas
topic_facet lipases
enantioselectivity
(R,S)-ibuprofen
description Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; Geotrichum candidum; Penicillium solitum) were compared in terms of their stability and as biocatalysts in the enantioselective esterification using racemic substrates in organic medium. The lipase from Aspergillus niger showed the highest activity (18.2 U/mL) and was highly thermostable, retaining 90% and 60% activity at 50 ºC and 60 ºC after 1 hour, respectively. In organic medium, this lipase provided the best results in terms of enantiomeric excess of the (S)-active acid (ee = 6.1%) and conversion value (c = 20%) in the esterification of (R,S)-ibuprofen with 1-propanol in isooctane. The esterification reaction of the racemic mixture of (R,S)-2-octanol with decanoic acid proceeded with high enantioselectivity when lipase from Aspergillus niger (E = 13.2) and commercial lipase from Candida antarctica (E = 20) were employed.
format Article in Journal/Newspaper
author Carvalho,Patrícia de O.
Calafatti,Silvana Ap.
Marassi,Maurício
Silva,Daniela M. da
Contesini,Fabiano J.
Bizaco,Renato
Macedo,Gabriela Alves
author_facet Carvalho,Patrícia de O.
Calafatti,Silvana Ap.
Marassi,Maurício
Silva,Daniela M. da
Contesini,Fabiano J.
Bizaco,Renato
Macedo,Gabriela Alves
author_sort Carvalho,Patrícia de O.
title Potencial de biocatálise enantiosseletiva de lipases microbianas
title_short Potencial de biocatálise enantiosseletiva de lipases microbianas
title_full Potencial de biocatálise enantiosseletiva de lipases microbianas
title_fullStr Potencial de biocatálise enantiosseletiva de lipases microbianas
title_full_unstemmed Potencial de biocatálise enantiosseletiva de lipases microbianas
title_sort potencial de biocatálise enantiosseletiva de lipases microbianas
publisher Sociedade Brasileira de Química
publishDate 2005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000400012
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Química Nova v.28 n.4 2005
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