Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology

The preparation of 2', 3'-di-O-hexanoyluridine (2) by a Candida antarctica B lipase-catalysed alcoholysis of 2', 3', 5'-tri-O-hexanoyluridine (1) was optimised using an experimental design. At 25 ºC better experimental conditions allowed an increase in the yield of 2 from 80...

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Bibliographic Details
Main Authors: Zinni,María A., Aljinovic,Ernesto M., Iglesias,Luis E., Iribarren,Adolfo M.
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Química 2004
Subjects:
enzymatic alcoholysis
experimental design
lipases
nucleosides
Antarc*
Antarctica
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422004000300022
id ftjscielo:oai:scielo:S0100-40422004000300022
record_format openpolar
spelling ftjscielo:oai:scielo:S0100-40422004000300022 2023-05-15T13:46:23+02:00 Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology Zinni,María A. Aljinovic,Ernesto M. Iglesias,Luis E. Iribarren,Adolfo M. 2004-06-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422004000300022 en eng Sociedade Brasileira de Química Química Nova v.27 n.3 2004 enzymatic alcoholysis experimental design lipases nucleosides journal article 2004 ftjscielo 2015-10-26T18:10:50Z The preparation of 2', 3'-di-O-hexanoyluridine (2) by a Candida antarctica B lipase-catalysed alcoholysis of 2', 3', 5'-tri-O-hexanoyluridine (1) was optimised using an experimental design. At 25 ºC better experimental conditions allowed an increase in the yield of 2 from 80% to 96%. In addition to the yield improvement, the volume reaction could be diminished in a factor of 5 and the reaction time significantly shortened. Article in Journal/Newspaper Antarc* Antarctica SciELO Brazil (Scientific Electronic Library Online)
institution Open Polar
collection SciELO Brazil (Scientific Electronic Library Online)
op_collection_id ftjscielo
language English
topic enzymatic alcoholysis
experimental design
lipases
nucleosides
spellingShingle enzymatic alcoholysis
experimental design
lipases
nucleosides
Zinni,María A.
Aljinovic,Ernesto M.
Iglesias,Luis E.
Iribarren,Adolfo M.
Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology
topic_facet enzymatic alcoholysis
experimental design
lipases
nucleosides
description The preparation of 2', 3'-di-O-hexanoyluridine (2) by a Candida antarctica B lipase-catalysed alcoholysis of 2', 3', 5'-tri-O-hexanoyluridine (1) was optimised using an experimental design. At 25 ºC better experimental conditions allowed an increase in the yield of 2 from 80% to 96%. In addition to the yield improvement, the volume reaction could be diminished in a factor of 5 and the reaction time significantly shortened.
format Article in Journal/Newspaper
author Zinni,María A.
Aljinovic,Ernesto M.
Iglesias,Luis E.
Iribarren,Adolfo M.
author_facet Zinni,María A.
Aljinovic,Ernesto M.
Iglesias,Luis E.
Iribarren,Adolfo M.
author_sort Zinni,María A.
title Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology
title_short Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology
title_full Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology
title_fullStr Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology
title_full_unstemmed Optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology
title_sort optimisation of the lipase-catalysed preparation of a nucleoside prodrug model using an experimental design methodology
publisher Sociedade Brasileira de Química
publishDate 2004
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422004000300022
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Química Nova v.27 n.3 2004
_version_ 1766241415172456448

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