Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens
We tested sera from 286 agricultural workers and 322 rodents in the department of Córdoba, northeastern Colombia, for antibodies against two hantaviruses. The sera were analysed by indirect ELISA using the lysate of Vero E6 cells infected with Maciel virus (MACV) or the N protein of Araraquara virus...
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Instituto Oswaldo Cruz, Ministério da Saúde
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ftjscielo:oai:scielo:S0074-02762013000200167 2023-05-15T18:05:29+02:00 Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens Guzmán,Camilo Mattar,Salim Levis,Silvana Pini,Noemí Figueiredo,Tadeu Mills,James Salazar-Bravo,Jorge 2013-04-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200167 en eng Instituto Oswaldo Cruz, Ministério da Saúde Memórias do Instituto Oswaldo Cruz v.108 n.2 2013 hantavirus Colombia epidemiological surveillance Rodentia public health immunoassay journal article 2013 ftjscielo 2015-10-26T17:37:33Z We tested sera from 286 agricultural workers and 322 rodents in the department of Córdoba, northeastern Colombia, for antibodies against two hantaviruses. The sera were analysed by indirect ELISA using the lysate of Vero E6 cells infected with Maciel virus (MACV) or the N protein of Araraquara virus (ARAV) as antigens for the detection of antibodies against hantaviruses. Twenty-four human sera were IgG positive using one or both antigens. We detected anti-MACV IgG antibodies in 10 sera (3.5%) and anti-ARAV antibodies in 21 sera (7.34%). Of the 10 samples that were positive for MACV, seven (70%) were cross-reactive with ARAV; seven of the 21 ARAV-positive samples were cross-reactive with MACV. Using an ARAV IgM ELISA, two of the 24 human sera (8.4%) were positive. We captured 322 rodents, including 210 Cricetidae (181 Zygodontomys brevicauda, 28 Oligoryzomys fulvescens and 1 Oecomys trinitatis), six Heteromys anomalus (Heteromyidae), one Proechimys sp. (Echimyidae) and 105 Muridae (34 Rattus rattus and 71 Mus musculus). All rodent sera were negative for both antigens. The 8.4% detection rate of hantavirus antibodies in humans is much higher than previously found in serosurveys in North America, suggesting that rural agricultural workers in northeastern Colombia are frequently exposed to hantaviruses. Our results also indicate that tests conducted with South American hantavirus antigens could have predictive value and could represent a useful alternative for the diagnosis of hantavirus infection in Colombia. Article in Journal/Newspaper Rattus rattus SciELO Brazil (Scientific Electronic Library Online) Maciel ENVELOPE(-67.850,-67.850,-66.833,-66.833) |
institution |
Open Polar |
collection |
SciELO Brazil (Scientific Electronic Library Online) |
op_collection_id |
ftjscielo |
language |
English |
topic |
hantavirus Colombia epidemiological surveillance Rodentia public health immunoassay |
spellingShingle |
hantavirus Colombia epidemiological surveillance Rodentia public health immunoassay Guzmán,Camilo Mattar,Salim Levis,Silvana Pini,Noemí Figueiredo,Tadeu Mills,James Salazar-Bravo,Jorge Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
topic_facet |
hantavirus Colombia epidemiological surveillance Rodentia public health immunoassay |
description |
We tested sera from 286 agricultural workers and 322 rodents in the department of Córdoba, northeastern Colombia, for antibodies against two hantaviruses. The sera were analysed by indirect ELISA using the lysate of Vero E6 cells infected with Maciel virus (MACV) or the N protein of Araraquara virus (ARAV) as antigens for the detection of antibodies against hantaviruses. Twenty-four human sera were IgG positive using one or both antigens. We detected anti-MACV IgG antibodies in 10 sera (3.5%) and anti-ARAV antibodies in 21 sera (7.34%). Of the 10 samples that were positive for MACV, seven (70%) were cross-reactive with ARAV; seven of the 21 ARAV-positive samples were cross-reactive with MACV. Using an ARAV IgM ELISA, two of the 24 human sera (8.4%) were positive. We captured 322 rodents, including 210 Cricetidae (181 Zygodontomys brevicauda, 28 Oligoryzomys fulvescens and 1 Oecomys trinitatis), six Heteromys anomalus (Heteromyidae), one Proechimys sp. (Echimyidae) and 105 Muridae (34 Rattus rattus and 71 Mus musculus). All rodent sera were negative for both antigens. The 8.4% detection rate of hantavirus antibodies in humans is much higher than previously found in serosurveys in North America, suggesting that rural agricultural workers in northeastern Colombia are frequently exposed to hantaviruses. Our results also indicate that tests conducted with South American hantavirus antigens could have predictive value and could represent a useful alternative for the diagnosis of hantavirus infection in Colombia. |
format |
Article in Journal/Newspaper |
author |
Guzmán,Camilo Mattar,Salim Levis,Silvana Pini,Noemí Figueiredo,Tadeu Mills,James Salazar-Bravo,Jorge |
author_facet |
Guzmán,Camilo Mattar,Salim Levis,Silvana Pini,Noemí Figueiredo,Tadeu Mills,James Salazar-Bravo,Jorge |
author_sort |
Guzmán,Camilo |
title |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_short |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_full |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_fullStr |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_full_unstemmed |
Prevalence of antibody to hantaviruses in humans and rodents in the Caribbean region of Colombia determined using Araraquara and Maciel virus antigens |
title_sort |
prevalence of antibody to hantaviruses in humans and rodents in the caribbean region of colombia determined using araraquara and maciel virus antigens |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2013 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200167 |
long_lat |
ENVELOPE(-67.850,-67.850,-66.833,-66.833) |
geographic |
Maciel |
geographic_facet |
Maciel |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Memórias do Instituto Oswaldo Cruz v.108 n.2 2013 |
_version_ |
1766176967576518656 |