The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus
Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral path...
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Instituto Oswaldo Cruz, Ministério da Saúde
2012
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ftjscielo:oai:scielo:S0074-02762012000100002 2023-05-15T15:58:46+02:00 The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus Corrêa,Adriana de Abreu Rigotto,Caroline Moresco,Vanessa Kleemann,Cristian Rafael Teixeira,Adriano Luiz Poli,Carlos Rogério Simões,Cláudia Maria Oliveira Barardi,Célia Regina Monte 2012-02-01 text/html http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000100002 en eng Instituto Oswaldo Cruz, Ministério da Saúde Memórias do Instituto Oswaldo Cruz v.107 n.1 2012 aquaculture depuration food safety shellfish viruses journal article 2012 ftjscielo 2015-10-26T17:37:01Z Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were contaminated with hepatitis A virus (HAV) or human adenovirus type 5 (HAdV5). After viral infection, the oysters were placed into a depuration tank and harvested after 48, 72 and 96 h. After sampling, various oyster tissues were dissected and homogenised and the viruses were eluted with alkaline conditions and precipitated with polyethylene glycol. The oyster samples were evaluated by cell culture methods, as well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover, at the end of the depuration period, the disinfected seawater was collected and analysed by PCR. The molecular assays showed that the HAdV5 genome was present in all of the depuration time samples, while the HAV genome was undetectable after 72 h of depuration. However, viral viability tests (integrated cell culture-PCR and immunofluorescence assay) indicated that both viruses were inactivated with 96 h of seawater recirculation. In conclusion, after 96 h of UV treatment, the depuration system studied in this work purified oysters that were artificially contaminated with HAdV5 and HAV. Article in Journal/Newspaper Crassostrea gigas SciELO Brazil (Scientific Electronic Library Online) |
institution |
Open Polar |
collection |
SciELO Brazil (Scientific Electronic Library Online) |
op_collection_id |
ftjscielo |
language |
English |
topic |
aquaculture depuration food safety shellfish viruses |
spellingShingle |
aquaculture depuration food safety shellfish viruses Corrêa,Adriana de Abreu Rigotto,Caroline Moresco,Vanessa Kleemann,Cristian Rafael Teixeira,Adriano Luiz Poli,Carlos Rogério Simões,Cláudia Maria Oliveira Barardi,Célia Regina Monte The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
topic_facet |
aquaculture depuration food safety shellfish viruses |
description |
Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were contaminated with hepatitis A virus (HAV) or human adenovirus type 5 (HAdV5). After viral infection, the oysters were placed into a depuration tank and harvested after 48, 72 and 96 h. After sampling, various oyster tissues were dissected and homogenised and the viruses were eluted with alkaline conditions and precipitated with polyethylene glycol. The oyster samples were evaluated by cell culture methods, as well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover, at the end of the depuration period, the disinfected seawater was collected and analysed by PCR. The molecular assays showed that the HAdV5 genome was present in all of the depuration time samples, while the HAV genome was undetectable after 72 h of depuration. However, viral viability tests (integrated cell culture-PCR and immunofluorescence assay) indicated that both viruses were inactivated with 96 h of seawater recirculation. In conclusion, after 96 h of UV treatment, the depuration system studied in this work purified oysters that were artificially contaminated with HAdV5 and HAV. |
format |
Article in Journal/Newspaper |
author |
Corrêa,Adriana de Abreu Rigotto,Caroline Moresco,Vanessa Kleemann,Cristian Rafael Teixeira,Adriano Luiz Poli,Carlos Rogério Simões,Cláudia Maria Oliveira Barardi,Célia Regina Monte |
author_facet |
Corrêa,Adriana de Abreu Rigotto,Caroline Moresco,Vanessa Kleemann,Cristian Rafael Teixeira,Adriano Luiz Poli,Carlos Rogério Simões,Cláudia Maria Oliveira Barardi,Célia Regina Monte |
author_sort |
Corrêa,Adriana de Abreu |
title |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_short |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_full |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_fullStr |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_full_unstemmed |
The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus |
title_sort |
depuration dynamics of oysters (crassostrea gigas) artificially contaminated with hepatitis a virus and human adenovirus |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2012 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000100002 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Memórias do Instituto Oswaldo Cruz v.107 n.1 2012 |
_version_ |
1766394535878852608 |